Difference between revisions of "Team:Warwick"

 
(130 intermediate revisions by 6 users not shown)
Line 1: Line 1:
−
 
 
<html>
 
<html>
−
<head>
+
  <head>
−
 
+
    <title>iGEM Warwick 2016 - Home</title>
−
<body style="background-color:MediumPurple;">
+
    <meta charset="utf-8" />
−
</head>
+
    <meta name="viewport" content="width=device-width, initial-scale=1" />
−
 
+
    <link rel="stylesheet" type="text/css" href="http:\\2016.igem.org/Team:Warwick\css?action=raw&ctype=text/css" />
−
 
+
  </head>
−
 
+
  <body>
−
<body>
+
    <header id="header">
−
 
+
        <div class="inner">
−
<br><br><img src="https://static.igem.org/mediawiki/2016/a/ae/WarwickCastleImage.jpeg" alt="Warwick Castle" style="width:950px;height:400px;">
+
        <a href="https://2016.igem.org/Team:Warwick" class="logo" >
−
 
+
            <img src="https://static.igem.org/mediawiki/2016/c/cd/T--Warwick--Logo.png" alt="IGEM WARWICK" class="logo">
−
</body>
+
        </a>
−
 
+
          <nav id="nav">
−
<br><h1 style="color:DarkSlateBlue;"> Project Description</h1>
+
            <a href="https://2016.igem.org/Team:Warwick">Home</a>
−
 
+
            <a href="https://2016.igem.org/Team:Warwick/Team">Team</a>
−
<p style="font-family: Century Gothic, CenturyGothic, AppleGothic, sans-serif""color:IndianRed;">
+
            <div class="dropdown">
−
There is no global challenge worth greater investment than improving quality of life. During initial discussions, we identified a wide range of problems that we felt further scientific development would be most beneficial: increasing crop yields; sewage filtration; reclamation of rare earth metals. Upon evaluation, the team decided that synthesizing a modular biosensor detection kit would be most influential, due to the potential application to a wide range of diseases and environmental health issues. This affordable, accessible system could tackle multiple issues over a large demographic, making the venture truly worthwhile. Lyme’s disease, although not at the forefront of discussion, is the inceptive focus of the investigation, as current detection methods can only be applied after debilitating symptoms have manifested. Our novel biosensor would therefore allow treatment of the disease at a period before serious symptoms have arisen. The theoretically adaptable nature of the bio detector also could allow replacement of antibody-reliant detection systems, hence why our other primary research focus is leptospirosis. We also aim to modify this technology such that it can be used to monitor toxic lead and mercury levels in water supplies.
+
            <a href="https://2016.igem.org/Team:Warwick/Description">Project</a>
 +
            <div class="dropdown-content">
 +
                <a href="https://2016.igem.org/Team:Warwick/Description">Description</a>
 +
                <a href="https://2016.igem.org/Team:Warwick/Design">Design</a>
 +
                <a href="https://2016.igem.org/Team:Warwick/Parts">Parts</a>
 +
                <a href="https://2016.igem.org/Team:Warwick/Model">Model</a>
 +
                <a href="https://2016.igem.org/Team:Warwick/Software">Software</a>
 +
            </div>
 +
            </div>
 +
            <div class="dropdown">
 +
            <a href="https://2016.igem.org/Team:Warwick/LabBook">Labs</a>
 +
            <div class="dropdown-content">
 +
                <a href="https://2016.igem.org/Team:Warwick/LabBook">Lab Book</a>
 +
                <a href="https://2016.igem.org/Team:Warwick/Safety">Safety</a>
 +
                <a href="https://2016.igem.org/Team:Warwick/Interlab">Interlab</a>
 +
                <a href="https://2016.igem.org/Team:Warwick/Protocols">Protocols</a>
 +
            </div>
 +
            </div>
 +
            <div class="dropdown">
 +
            <a href="https://2016.igem.org/Team:Warwick/Attributions">Attributions</a>
 +
            <div class="dropdown-content">
 +
                <a href="https://2016.igem.org/Team:Warwick/Collaborations">Collaborations</a>
 +
            </div>
 +
            </div>
 +
            <div class="dropdown">
 +
            <a href="https://2016.igem.org/Team:Warwick/Human_Practices">Human Practices</a>
 +
            <div class="dropdown-content">
 +
                <a href="https://2016.igem.org/Team:Warwick/Human_Practices">Summary</a>
 +
                <a href="https://2016.igem.org/Team:Warwick/HP/Silver">Silver</a>
 +
                <a href="https://2016.igem.org/Team:Warwick/HP/Gold">Gold</a>
 +
                <a href="https://2016.igem.org/Team:Warwick/Integrated_Practices">Integrated Practices</a>
 +
                <a href="https://2016.igem.org/Team:Warwick/Education">Education</a>
 +
            </div> 
 +
            </div>
 +
          </nav>
 +
          <a href="#navPanel" class="navPanelToggle"><span class="fa fa-bars"></span></a>
 +
        </div>
 +
    </header>
  
−
<br><br>Our detection system for infectious agents and environmental pollutants will be based on CRISPR/Cas9 technology, relying on conformational changes in an RNA-based sensor that will trigger transcriptional regulation by a dCas9 protein. We plan to produce a modular gene circuit that can detect the presence of either RNA from an infectious agent or metallic ion and output a fluorescent signal. This will be arranged on a plasmid encoding for an RNA sensor, the CRISPR/Cas9 system, and a fluorescent reporter. The RNA sensor will be a modified sgRNA containing a motif binding either Borrelia/Leptospira RNA or lead/mercury aptamers. This binding will cause a conformational change such that the modified dCas9 enzyme may bind upstream of the transcriptional start site of the fluorescent reporter gene inducing transcription. We will be testing this using multiple novel dCas9 fusion proteins as well as more traditional CRISPR/dCas9 methods. Afterwards, the sensor will be freeze-dried onto a paper scaffold for ease of use.
+
     
  
−
<br><br>An example of a modified CRISPR/dCas9 system to be used in the project.
+
    <!-- Banner -->
−
By the end of the project we hope to create a paper based sensor that will be able to be used in a low-tech, out-of-lab, environment. We hope this will make it available to less economically developed countries as a frontline diagnostic tool and make a real impact in the fight against infectious diseases and pollution.</p>
+
      <section id="banner">
 +
        <h1>Warwick iGEM 2016</h1>
 +
        <p>Application of CRISPR/Cas9 as a novel modular biosensor</p>
 +
      </section>
  
 +
    <!-- One -->
 +
      <section id="one" class="wrapper">
 +
        <div class="inner">
 +
          <div class="flex flex-3">
 +
            <article>
 +
              <header>
 +
                <h3>CRISPR/Cas9<br /> Biosensor.</h3>
 +
              </header>
 +
              <p>Using a dCas9 protein and an RNA-binding protein (RBP) fused to an effector, transcription of a fluorescent reporter gene can be regulated, with a significant colour change indicating the presence of targeted RNA.</p>
 +
            </article>
 +
            <article>
 +
              <header>
 +
                <h3>Leptospirosis Impact and Diagnosis.</h3>
 +
              </header>
 +
              <p>Our project will enable diagnosis of the disease at an early stage, increasing the likelihood of successful treatment and reducing the risk of irreversible bodily damage.</p>
 +
            </article>
 +
            <article>
 +
              <header>
 +
                <h3>Pollutant Detection: Heavy Metal Ions.</h3>
 +
              </header>
 +
              <p>Our team has developed a system capable of detecting these ions in water sources, potentially improving the quality of human life on a global scale, in an inexpensive easily accessible manner.</p>
 +
            </article>
 +
          </div>
 +
        </div>
 +
      </section>
 +
     
 +
     
 +
     
 +
     
 +
  <!-- Footer -->
 +
    <footer id="footer">
 +
              <div class="inner">
 +
              <div class="flex">
 +
                <div class="copyright">
 +
                  &copy; Warwick iGEM 2016.
 +
                <ul class="icons">
 +
                    <li><a href="https://www.facebook.com/WarwickIGEM" class="logo" ><img src="https://static.igem.org/mediawiki/2016/6/6f/T--Warwick--Facebook.png" alt="Facebook" class="logo">
 +
                    </a></li>
 +
                    <li><a href="https://twitter.com/warwickigem" class="logo" ><img src="https://static.igem.org/mediawiki/2016/7/7e/T--Warwick--Twitter.png" alt="Twitter" class="logo">
 +
                    </a></li>
 +
                </ul>
 +
                <p>We thankfully acknowledge generous funding support from our sponsors below.</p>
 +
                <a href="https://2016.igem.org/Team:Warwick/Attributions" class="logo" >
 +
                    <img src="https://static.igem.org/mediawiki/2016/f/f6/T--Warwick--banner.png" alt="IGEM WARWICK" class="bannertop">
 +
                </a>
 +
              </div>
 +
            </div>
 +
          </div>
 +
      </footer>
 +
  </body>
 
</html>
 
</html>

Latest revision as of 02:26, 20 October 2016

iGEM Warwick 2016 - Home

CRISPR/Cas9
Biosensor.

Using a dCas9 protein and an RNA-binding protein (RBP) fused to an effector, transcription of a fluorescent reporter gene can be regulated, with a significant colour change indicating the presence of targeted RNA.

Leptospirosis Impact and Diagnosis.

Our project will enable diagnosis of the disease at an early stage, increasing the likelihood of successful treatment and reducing the risk of irreversible bodily damage.

Pollutant Detection: Heavy Metal Ions.

Our team has developed a system capable of detecting these ions in water sources, potentially improving the quality of human life on a global scale, in an inexpensive easily accessible manner.

© Warwick iGEM 2016.

We thankfully acknowledge generous funding support from our sponsors below.