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| <div class="container-fluid member-head"> | | <div class="container-fluid member-head"> |
| <div class="container"> | | <div class="container"> |
− | <h2 class="text-center">Proof Of Concept</h2> | + | <h2 class="text-center">Proof of Concept</h2> |
| </div> | | </div> |
| </div> | | </div> |
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| <h3>Hello world</h3> | | <h3>Hello world</h3> |
| <p>fdwfewf</p> --> | | <p>fdwfewf</p> --> |
− | <h2 id="location_Pro_ver">Prokaryotic version:</h2> | + | <h2 id="location_Pro_ver">Prokaryotic version</h2> |
| <p> | | <p> |
| It is a tri-stable system adapted from bacteriophage λ operon. In the operon, promoter RE is activated by CII trascriptional activator. Ftsh is an ATP-dependent host metalloprotease which will normally degrade CII, while CIII serves as an inhibitor of Ftsh to free CII. CI can function as an inhibitor to block pR, while Cro can bind pRM to stop the expression of downstream gene. The positive feedback control under pRE is used to enhance pulse signal 1 and convert it into robust stable signal. | | It is a tri-stable system adapted from bacteriophage λ operon. In the operon, promoter RE is activated by CII trascriptional activator. Ftsh is an ATP-dependent host metalloprotease which will normally degrade CII, while CIII serves as an inhibitor of Ftsh to free CII. CI can function as an inhibitor to block pR, while Cro can bind pRM to stop the expression of downstream gene. The positive feedback control under pRE is used to enhance pulse signal 1 and convert it into robust stable signal. |
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| <div class="row"> | | <div class="row"> |
| <div class="col-md-6" style="margin:100px 0"> | | <div class="col-md-6" style="margin:100px 0"> |
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| <p style="margin-left:100px"> | | <p style="margin-left:100px"> |
| According to the Flourescence measurement curve, we can see clearly that GFP level increased over time and it showed significant difference from CK. See more details in our Results page. | | According to the Flourescence measurement curve, we can see clearly that GFP level increased over time and it showed significant difference from CK. See more details in our Results page. |
| </p> | | </p> |
− | <p><a href="https://2016.igem.org/Team:HUST-China/Results" class="btn btn-primary" role="button">More details</a></p> | + | <p><a href="https://2016.igem.org/Team:HUST-China/Results#location_pro&pro" class="btn btn-primary" role="button">More details</a></p> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="row"> | | <div class="row"> |
| <div class="col-md-6" style="margin:100px 0"> | | <div class="col-md-6" style="margin:100px 0"> |
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| <p style="margin-left:70px"> | | <p style="margin-left:70px"> |
| We did Fluorescence microscope detection after 30, 120 and 240 minutes induction. According to the figture below, we can tell qualitively that pRE leakage are at relative low level and CII can efficiently activate the promoter. See more details in our Results page. | | We did Fluorescence microscope detection after 30, 120 and 240 minutes induction. According to the figture below, we can tell qualitively that pRE leakage are at relative low level and CII can efficiently activate the promoter. See more details in our Results page. |
| </p> | | </p> |
− | <p><a href="https://2016.igem.org/Team:HUST-China/Results" class="btn btn-primary" role="button">More details</a></p> | + | <p><a href="https://2016.igem.org/Team:HUST-China/Results#location_pro&pro" class="btn btn-primary" role="button">More details</a></p> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="row"> | | <div class="row"> |
| <div class="col-md-6" style="margin:100px 0"> | | <div class="col-md-6" style="margin:100px 0"> |
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| <p style="margin-left:100px"> | | <p style="margin-left:100px"> |
| As the Relative Fluorescent intensity measurement data shows, CI can inhibit pR in minor degree but the leakage expression under pR can’t be ignored, so we should consider to increase the binding sites within pR or the amount of CI coding sequence in the circuit. See more details in our Results page. | | As the Relative Fluorescent intensity measurement data shows, CI can inhibit pR in minor degree but the leakage expression under pR can’t be ignored, so we should consider to increase the binding sites within pR or the amount of CI coding sequence in the circuit. See more details in our Results page. |
| </p> | | </p> |
− | <p><a href="https://2016.igem.org/Team:HUST-China/Results" class="btn btn-primary" role="button">More details</a></p> | + | <p><a href="https://2016.igem.org/Team:HUST-China/Results#location_pro&pro" class="btn btn-primary" role="button">More details</a></p> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="row"> | | <div class="row"> |
| <div class="col-md-6" style="margin:100px 0"> | | <div class="col-md-6" style="margin:100px 0"> |
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| <p style="margin-left:100px"> | | <p style="margin-left:100px"> |
| We detected GFP reporter in E.coli after induction of 20minute, 120minutes and 240minutes through 20 times of amplification. See more details in our Results page. | | We detected GFP reporter in E.coli after induction of 20minute, 120minutes and 240minutes through 20 times of amplification. See more details in our Results page. |
| </p> | | </p> |
− | <p><a href="https://2016.igem.org/Team:HUST-China/Results" class="btn btn-primary" role="button">More details</a></p> | + | <p><a href="https://2016.igem.org/Team:HUST-China/Results#location_pro&pro" class="btn btn-primary" role="button">More details</a></p> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="row"> | | <div class="row"> |
| <div class="col-md-6" style="margin:100px 0"> | | <div class="col-md-6" style="margin:100px 0"> |
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| <p style="margin-left:100px"> | | <p style="margin-left:100px"> |
| We characterized cro and pRM inhibition by the same method as CI and pR’s. See more details in our Results page. | | We characterized cro and pRM inhibition by the same method as CI and pR’s. See more details in our Results page. |
| </p> | | </p> |
− | <p><a href="https://2016.igem.org/Team:HUST-China/Results" class="btn btn-primary" role="button">More details</a></p> | + | <p><a href="https://2016.igem.org/Team:HUST-China/Results#location_pro&pro" class="btn btn-primary" role="button">More details</a></p> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="row"> | | <div class="row"> |
| <div class="col-md-6" style="margin:100px 0"> | | <div class="col-md-6" style="margin:100px 0"> |
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| <p style="margin-left:100px"> | | <p style="margin-left:100px"> |
| We use IPTG with final concentration of 1mM to induce the GFP-LVAssrAtag and measure the relative fluorescence through plate reader with Excitation light 495nm. See more details in our Results page. | | We use IPTG with final concentration of 1mM to induce the GFP-LVAssrAtag and measure the relative fluorescence through plate reader with Excitation light 495nm. See more details in our Results page. |
| </p> | | </p> |
− | <p><a href="https://2016.igem.org/Team:HUST-China/Results" class="btn btn-primary" role="button">More details</a></p> | + | <p><a href="https://2016.igem.org/Team:HUST-China/Results#location_tri_fun" class="btn btn-primary" role="button">More details</a></p> |
| </div> | | </div> |
| </div> | | </div> |
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− | <h2 id="location_Euk_ver">Eukaryotic version:</h2> | + | <h2 id="location_Euk_ver">Eukaryotic version</h2> |
| <p>It is a bi-stable system derived from Arabidopsis thaliana stress response system. Enzyme catalysis is the core of this design to increase the efficiency of state transition. | | <p>It is a bi-stable system derived from Arabidopsis thaliana stress response system. Enzyme catalysis is the core of this design to increase the efficiency of state transition. |
| </p> | | </p> |
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| We ran a SDS-PAGE of the culture supernatants of induced cells to identify the three recombinant proteins. See more details in our Results page. | | We ran a SDS-PAGE of the culture supernatants of induced cells to identify the three recombinant proteins. See more details in our Results page. |
| </p> | | </p> |
− | <p><a href="https://2016.igem.org/Team:HUST-China/Results" class="btn btn-primary" role="button">More details</a></p> | + | <p><a href="https://2016.igem.org/Team:HUST-China/Results#location_pro_exp" class="btn btn-primary" role="button">More details</a></p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
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− | <div class="container">
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− | <div class="row">
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− | <div class="col-md-6" style="margin:100px 0">
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− | <div class="caption">
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− | <p style="margin-left:70px">
| + | <p> |
− | We ran a SDS-PAGE of the culture supernatants of induced cells to identify the three recombinant proteins. See more details in our Results page.
| + | We simulated our project in cellular level and Intestinal level to help us figure out the feasibility of it and guide us the direction of further improvement. See more results in our Model page. |
− | </p>
| + | </p> |
− | <p><a href="https://2016.igem.org/Team:HUST-China/Results" class="btn btn-primary" role="button">More details</a></p>
| + | <p><a href="https://2016.igem.org/Team:HUST-China/Model" class="btn btn-primary" role="button">More details</a></p> |
− | </div>
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− | </div>
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− | <div class="col-md-6" style="margin:100px 0">
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− | <img src="https://static.igem.org/mediawiki/2016/b/b1/T--HUST-China--SDS.png" alt="" class="img-responsive">
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− | </div>
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− | </div>
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| </div> | | </div> |
| + | </div> |
| + | <div class="col-md-6" style="margin:100px 0"> |
| + | <img src="https://static.igem.org/mediawiki/2016/5/59/T--HUST-China--modeling-application-LA_decomposition.png" alt="" class="img-responsive"> |
| </div> | | </div> |
| </div> | | </div> |
| + | </div> |
| + | </div> |
| + | </div> |
| <!-- 轮播(Carousel)导航 --> | | <!-- 轮播(Carousel)导航 --> |
| <a class="carousel-control left" href="#myCarousel2" data-slide="prev" style="font-size:100px;">‹</a> | | <a class="carousel-control left" href="#myCarousel2" data-slide="prev" style="font-size:100px;">‹</a> |