Difference between revisions of "Team:Harvard BioDesign"

 
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<h3>Project Description</h3>
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The Harvard iGEM team began our brainstorming sessions during the late spring. We had a massive brainstorming session where we generated topics to investigate, and in the following weeks, scoured the internet for previous research relating to these topics. We met a few more times to discuss what we had found. After narrowing our search down to 4 projects, we wrote mini proposals to present each idea to the rest of the team. At the beginning of the summer, we continued our project planning with one project: Breaking PET.  
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In Breaking PET, we hope to engineer a biological system that breaks down PET, a molecule which makes up one sixth of plastic products (source). Our project is grounded in research done by the Oda group at Kyoto Institute of Technology. In the paper “A bacteria that degrades and assimilates poly(ethylene terephthalate)”, they describe a bacteria found at a bottle recycling plant that has the ability to metabolize PET. From this paper, we found enzyme sequences for the two enzymes thought responsible for PET degradation: PETase and MHETase. PETase breaks down PET into the compound MHET, or mono(2-hydroxylethyl)terephthalic acid. MHETase further breaks down MHET into terephthalic acid and ethylene glycol. The discovery of these two enzymes is exciting because, unlike other PET degrading enzymes, PETase and MHETase are dedicated to the role of PET degradation. Additionally, PETase has been shown to have a degrading efficiency 120 times greater than alternative enzymes. (source)
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Our project aims to use E Coli transformed with PETase and MHETase to break down PET and generate electricity. We will create BioBricks for PETase and MHETase and characterize these parts against enzymes used in previous PET degrading iGEM projects. Additionally, we hope to engineer a secretion system for these enzymes. We will also attempt to make a bioreactor/microbial fuel cell to generate electricity from the products of the PET degradation process.  
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<h2> Welcome to iGEM 2016! </h2>
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<h1><a href="https://2016.igem.org/Team:Harvard_BioDesign" id="logo">Harvard BioDesign</a></h1>
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<p>Description</p>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign">Home</a></li>
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<li> <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<a href="#">Team</a>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Team">Team</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Collaborations">Collaborations</a></li>
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<a href="#">Project</a>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Description">Description</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Design">Design</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Proof">Proof of Concept</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Notebook">Notebook</a></li>
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<a href="#">Parts</a>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Parts">Parts</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Basic_Part">Basic Parts</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Safety">Safety</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Attributions">Attributions</a></li>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<a href="#">Human Practices</a>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Human_Practices">Human Practices</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/HP/Silver">Silver</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/HP/Gold">Gold</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Integrated_Practices">Integrated Practices</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Engagement">Engagement</a></li>
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<a href="#">Awards</a>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Model">Model</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Achievements">Achievements</a></li>
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<h2>Harvard BioDesign 2016.</h2>
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<h5> Editing your wiki </h5>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
 
  
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
  
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UPLOAD FILES
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<h2>Plastiback</h2>
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<h3>A synthetic biology based plastic sensing feedback device.</h3>
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<h4>Degrade plastic more simply, efficiently, sustainably. Anywhere.</h4>
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<h4>Generate electricity from degradation to map out plastic in the ocean.</h4>
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<section><p><center>
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Plastiback is a plastic-sensing system that gives feedback on where PET, a type of plastic, is located in the ocean.
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<br>PET is a durable plastic, with multiple uses, including making the bottles for many of the drinks we enjoy. While PET pollutes both the land and sea, the majority of PET litter ends up in the ocean. When plastic enters the ocean, it becomes difficult to clean up and even more difficult to track. For researchers who are investigating the scope and map of where PET is, Plastiback presents a novel and sustainable way to locate ocean PET, while also using non-toxic methods to degrade PET!</center>
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</p></section>
  
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<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/d/d6/T--Harvard_BioDesign--home_davidrebekahfinal.png/1600px-T--Harvard_BioDesign--home_davidrebekahfinal.png"/></a>
  
  
  
  
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<h3>Achievements</h3>
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Latest revision as of 03:36, 20 October 2016

Harvard BioDesign 2016

Plastiback

A synthetic biology based plastic sensing feedback device.

Degrade plastic more simply, efficiently, sustainably. Anywhere.

Generate electricity from degradation to map out plastic in the ocean.

Plastiback is a plastic-sensing system that gives feedback on where PET, a type of plastic, is located in the ocean.
PET is a durable plastic, with multiple uses, including making the bottles for many of the drinks we enjoy. While PET pollutes both the land and sea, the majority of PET litter ends up in the ocean. When plastic enters the ocean, it becomes difficult to clean up and even more difficult to track. For researchers who are investigating the scope and map of where PET is, Plastiback presents a novel and sustainable way to locate ocean PET, while also using non-toxic methods to degrade PET!

Team

Project

Parts

Safety

Attributions

Human Practices

Achievements