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− | {{:Team:Tianjin/Templates/AddCSS|:Team:Tianjin/Note/Consortium/camarts.css}} | + | {{:Team:Tianjin/Templates/AddCSS|:Team:Tianjin/Note/R-R/camarts.css}} |
| {{:Team:Tianjin/Templates/AddCSS|:Team:Tianjin/Experiment/css/font.css}} | | {{:Team:Tianjin/Templates/AddCSS|:Team:Tianjin/Experiment/css/font.css}} |
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| <html lang="en"> | | <html lang="en"> |
| + | <style> |
| + | #Notes{ |
| + | color: inherit; |
| + | background-color: rgba(255, 255, 255, 0.1); |
| + | } |
| + | </style> |
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| <meta content="width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0" name="viewport" /> | | <meta content="width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0" name="viewport" /> |
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| + | <style> |
| + | #Notes{ |
| + | color: inherit; |
| + | background-color: rgba(255, 255, 255, 0.1); |
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| + | </style> |
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| <!------------------------------------------- This part should be dismissed in WIKI u | | <!------------------------------------------- This part should be dismissed in WIKI u |
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| <article id="post-4252" class="post-4252 post type-post status-publish format-standard has-post-thumbnail hentry category-150 tag-174 tag-xinjiang tag-173"> | | <article id="post-4252" class="post-4252 post type-post status-publish format-standard has-post-thumbnail hentry category-150 tag-174 tag-xinjiang tag-173"> |
| <header class="entry-header"> | | <header class="entry-header"> |
− | <div class="entry-title" align="center" >Notes For Modified Cyanobacteria:A Controllable Lipid Producer</div> | + | <div class="entry-title" align="center" >Notes For Cyanobacteria</div> |
| </header><!-- .entry-header --> | | </header><!-- .entry-header --> |
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− | <div align="center">
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− | <figure>
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− | <a href="https://static.igem.org/mediawiki/2016/3/37/Igem--6803-week2.jpg" data-lightbox="no" data-title="Fig.3 Proposed degradation pathway for TPA<sup>[2]</sup>"><img src="https://static.igem.org/mediawiki/2016/3/37/Igem--6803-week2.jpg" width="100%"></a>
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− | <figcation>Fig.3 Proposed degradation pathway for TPA<sup>[2]</sup></figcaption>
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− | </figure>
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− | </div>
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| + | <a href="https://static.igem.org/mediawiki/2016/3/37/Igem--6803-week2.jpg" data-lightbox="no" data-title="7942"> |
| <img align="center" src="https://static.igem.org/mediawiki/2016/3/37/Igem--6803-week2.jpg" alt="Igem-6803-week2" width="300px"><br/><br/> | | <img align="center" src="https://static.igem.org/mediawiki/2016/3/37/Igem--6803-week2.jpg" alt="Igem-6803-week2" width="300px"><br/><br/> |
| </a> | | </a> |
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| <div style="padding-left:32px;"><li>pMV-G19 and pMV-G15 containing our target genes were received.</li><br/> | | <div style="padding-left:32px;"><li>pMV-G19 and pMV-G15 containing our target genes were received.</li><br/> |
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| + | <a href="https://static.igem.org/mediawiki/2016/6/6d/Igem-6803-week3-1.jpg" data-lightbox="no" data-title="pMV-G19 and pMV-G15"> |
| <img align="center" src="https://static.igem.org/mediawiki/2016/6/6d/Igem-6803-week3-1.jpg" alt="Igem-6803-week3-1" width="300px" ><br/><br/> | | <img align="center" src="https://static.igem.org/mediawiki/2016/6/6d/Igem-6803-week3-1.jpg" alt="Igem-6803-week3-1" width="300px" ><br/><br/> |
| </a> | | </a> |
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| <li>The enzyme-digested product was dephosphorylation.</li> | | <li>The enzyme-digested product was dephosphorylation.</li> |
| <br/> | | <br/> |
| + | |
| + | <a href="https://static.igem.org/mediawiki/2016/a/aa/Igem-6803-week3.png" data-lightbox="no" data-title=" "> |
| <img align="center" src="https://static.igem.org/mediawiki/2016/a/aa/Igem-6803-week3.png" alt="Igem-6803-week3-2" width="800" height="533"><br/> | | <img align="center" src="https://static.igem.org/mediawiki/2016/a/aa/Igem-6803-week3.png" alt="Igem-6803-week3-2" width="800" height="533"><br/> |
| </a> | | </a> |
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| Two of the successful ones were used to inoculate overnight cultures.<br/> | | Two of the successful ones were used to inoculate overnight cultures.<br/> |
| <br/> | | <br/> |
| + | |
| + | <a href="https://static.igem.org/mediawiki/2016/8/81/Igem-6803-week4.png" data-lightbox="no" data-title=" "> |
| <img align="center" src="https://static.igem.org/mediawiki/2016/8/81/Igem-6803-week4.png" alt="Igem-6803-week4" width="800" height="533"> <br/> | | <img align="center" src="https://static.igem.org/mediawiki/2016/8/81/Igem-6803-week4.png" alt="Igem-6803-week4" width="800" height="533"> <br/> |
| </a> | | </a> |
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| <b>The fourth and sixth ones were not successful.</b><br/> | | <b>The fourth and sixth ones were not successful.</b><br/> |
| <br/> | | <br/> |
| + | |
| + | <a href="https://static.igem.org/mediawiki/2016/4/40/Igem-6803-week5.png" data-lightbox="no" data-title=" "> |
| <img align="center"src="https://static.igem.org/mediawiki/2016/4/40/Igem-6803-week5.png" alt="Igem-6803-week5" width="800" height="533" ><br/> | | <img align="center"src="https://static.igem.org/mediawiki/2016/4/40/Igem-6803-week5.png" alt="Igem-6803-week5" width="800" height="533" ><br/> |
| </a> | | </a> |
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| <b><h1 style="font-size:108%"> Sep.22th</b></h1> | | <b><h1 style="font-size:108%"> Sep.22th</b></h1> |
− | <div style="padding-left:32px;"><li>Gel electrophoresis showed that amplification of fragments was successfull.</li> | + | <div style="padding-left:32px;"> |
| + | <li>Gel electrophoresis showed that amplification of fragments was successfull.</li> |
| <li>Ligated <i>13</i> and <i>19-15</i> via overlap PCR.</li> | | <li>Ligated <i>13</i> and <i>19-15</i> via overlap PCR.</li> |
| <li>This PCR worked well and the products were extracted from the gel using a Agarose Gel DNA Extration Kit.</li> | | <li>This PCR worked well and the products were extracted from the gel using a Agarose Gel DNA Extration Kit.</li> |
| <li>Restriction digest on pCPC-3031-Ni with Sac I.</li> | | <li>Restriction digest on pCPC-3031-Ni with Sac I.</li> |
| <br/> | | <br/> |
− | <img align="center" src="https://static.igem.org/mediawiki/2016/8/89/Igem-6803-week6-2.jpg" alt="Igem-6803-week6-1" width="300px"><br/> | + | <a href="https://static.igem.org/mediawiki/2016/8/89/Igem-6803-week6-2.jpg" data-lightbox="no" data-title=" "> |
− | <br/>
| + | <img align="center" src="https://static.igem.org/mediawiki/2016/8/89/Igem-6803-week6-2.jpg" alt="Igem-6803-week6-1" width="300px"> |
| + | </a> |
| + | |
| <li>The fragment <i>13-19-15</i> was ligated onto T vector and transformed into E.coli via heat shock.</li> | | <li>The fragment <i>13-19-15</i> was ligated onto T vector and transformed into E.coli via heat shock.</li> |
− | <br/> | + | |
− | <img align="center" src="https://static.igem.org/mediawiki/2016/0/06/Igem-6803-week6.jpg" alt="Igem-6803-week6-2" width="300px" ><br/> | + | <a href="https://static.igem.org/mediawiki/2016/0/06/Igem-6803-week6.jpg" data-lightbox="no" data-title=" "> |
| + | <img align="center" src="https://static.igem.org/mediawiki/2016/0/06/Igem-6803-week6.jpg" alt="Igem-6803-week6-2" width="300px" > |
| + | </a> |
| <br/> | | <br/> |
| </div> | | </div> |
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| <li>Three colonies were proved to be true. And two of them were used to inoculate overnight cultures.</li> | | <li>Three colonies were proved to be true. And two of them were used to inoculate overnight cultures.</li> |
| <br/> | | <br/> |
| + | <a href="https://static.igem.org/mediawiki/2016/2/2d/Igem-6803-week6-3.png" data-lightbox="no" data-title=" "> |
| <img align="center" src="https://static.igem.org/mediawiki/2016/2/2d/Igem-6803-week6-3.png" alt="Igem-6803-week6-3" width="800" height="533" ><br/> | | <img align="center" src="https://static.igem.org/mediawiki/2016/2/2d/Igem-6803-week6-3.png" alt="Igem-6803-week6-3" width="800" height="533" ><br/> |
| + | </a> |
| <br/> | | <br/> |
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| </div> | | </div> |
| <br/> | | <br/> |
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| <b><h1 style="font-size:108%"> Sep.26th</b></h1> | | <b><h1 style="font-size:108%"> Sep.26th</b></h1> |
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| <br/> | | <br/> |
| </div> | | </div> |
| + | </br> |
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| + | <b><h1 style="font-size:108%"> Sep.28th</b></h1> |
| + | <div style="padding-left:32px;"><li>We transformed the expression vector, pCPC-3031-Ni-13-19-15, into <i>Synechocystis 6803 </i>via electroporation.</li> |
| <br/> | | <br/> |
| + | <b>The story of 6803 is not over. Other team members will show the completed process during the presentation. </br> |
| + | I will cherish the time spending with you for my whole life. ———by Jiawei Chen and Liangyu Qian</b> |
| + | </div> |
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| </div> | | </div> |
− | <div class="side-nav">
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− | <ul>
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− | <li><a class="topLink" href="#Topp" style="color:#5555FF">Top</a></li>
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− | <li><a class="topLink" href="#Week1" style="color:#5555FF">Week1</a></li>
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− | <li><a class="topLink" href="#Week2" style="color:#5555FF">Week2</a></li>
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− | <li><a class="topLink" href="#Week3" style="color:#5555FF">Week3</a></li>
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− | <li><a class="topLink" href="#Week4" style="color:#5555FF">Week4</a></li>
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− | <li><a class="topLink" href="#Week5" style="color:#5555FF">Week5</a></li>
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− | <li><a class="topLink" href="#Week6" style="color:#5555FF">Week6</a></li>
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− | <li><a class="topLink" href="#Week7" style="color:#5555FF">Week7</a></li>
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− | </ul>
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− | </div>
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