Difference between revisions of "Team:Harvard BioDesign/Composite Part"

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<h1><a href="https://2016.igem.org/Team:Harvard_BioDesign" id="logo">Harvard BioDesign</a></h1>
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<p>Description</p>
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<header>
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<h2>Composite Parts</h2>
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<h3> Composite Parts </h3>
  
 
<p>
 
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<a href="https://2016.igem.org/Team:Harvard_BioDesign/Composite_Part">Composite Parts</a>
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are parts made of basic parts that combine the functions of each basic part to provide a higher-order function, such as the production PETase.
 
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<p>The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon.</p>
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<p>Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase.</p>
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<section>
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<p>Overall, there are 8 composite parts, which consist of the following:</p>
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<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/d/dc/T--Harvard_BioDesign--BBa_K2010000.png/1600px-T--Harvard_BioDesign--BBa_K2010000.png"/></a>
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<p><a href="http://parts.igem.org/Part:BBa_K2010000"><b>BBa_K201000:</b> T7 Promoter + PETase + His-Tag + Stop Codon</a></p>
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<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/b/be/T--Harvard_BioDesign--BBa_K2010001.png/1600px-T--Harvard_BioDesign--BBa_K2010001.png"/></a>
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<p><a href="http://parts.igem.org/Part:BBa_K2010001"><b>BBa_K201001:</b> T7 Promoter + ompT + PETase + His-Tag + Stop Codon</a></p>
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<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/9/94/T--Harvard_BioDesign--BBa_K2010002.png/1596px-T--Harvard_BioDesign--BBa_K2010002.png"/></a>
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<p><a href="http://parts.igem.org/Part:BBa_K2010002"><b>BBa_K201002:</b> T7 Promoter + pelB + PETase + His-Tag + Stop Codon</a></p>
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<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/1/19/T--Harvard_BioDesign--BBa_K2010003.png/1600px-T--Harvard_BioDesign--BBa_K2010003.png"/></a>
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<p><a href="http://parts.igem.org/Part:BBa_K2010003"><b>BBa_K201003:</b> T7 Promoter + YebF + PETase + His-Tag + Stop Codon</a></p>
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<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/6/6d/T--Harvard_BioDesign--BBa_K2010004.png/1600px-T--Harvard_BioDesign--BBa_K2010004.png"/></a>
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<p><a href="http://parts.igem.org/Part:BBa_K2010004"><b>BBa_K201004:</b> T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon</a></p>
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<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/9/90/T--Harvard_BioDesign--BBa_K2010005.png/1598px-T--Harvard_BioDesign--BBa_K2010005.png"/></a>
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<p><a href="http://parts.igem.org/Part:BBa_K2010005"><b>BBa_K201005:</b> T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon</a></p>
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<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/d/d3/T--Harvard_BioDesign--BBa_K2010007.png/1598px-T--Harvard_BioDesign--BBa_K2010007.png"/></a>
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<p><a href="http://parts.igem.org/Part:BBa_K2010007"><b>BBa_K201007:</b> T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon</a></p>
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<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/c/c9/T--Harvard_BioDesign--BBa_K2010008.png/1595px-T--Harvard_BioDesign--BBa_K2010008.png"/></a>
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<p><a href="http://parts.igem.org/Part:BBa_K2010008"><b>BBa_K201008:</b> T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon</a></p>
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<h4>Note</h4>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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Latest revision as of 12:49, 19 January 2017

Harvard BioDesign 2016

Composite Parts

Composite Parts

Composite Parts are parts made of basic parts that combine the functions of each basic part to provide a higher-order function, such as the production PETase.

The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon.

Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase.

Overall, there are 8 composite parts, which consist of the following:

BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon

BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon

BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon

BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon

BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon

BBa_K201005: T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon

BBa_K201007: T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon

BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon