Difference between revisions of "Team:Harvard BioDesign/Composite Part"

(Removed hyperlink in page title)
 
(One intermediate revision by one other user not shown)
Line 120: Line 120:
 
<article id="main" class="special">
 
<article id="main" class="special">
 
<header>
 
<header>
−
<h2><a href="#">Composite Parts</a></h2>
+
<h2>Composite Parts</h2>
 
 
  
Line 129: Line 129:
  
 
<p>
 
<p>
−
<a href="https://2016.igem.org/Team:Harvard_BioDesign/Composite_Part">Composite Parts</a></p>
+
<a href="https://2016.igem.org/Team:Harvard_BioDesign/Composite_Part">Composite Parts</a>
 
  are parts made of basic parts that combine the functions of each basic part to provide a higher-order function, such as the production PETase.
 
  are parts made of basic parts that combine the functions of each basic part to provide a higher-order function, such as the production PETase.
 
</p>
 
</p>

Latest revision as of 12:49, 19 January 2017

Harvard BioDesign 2016

Composite Parts

Composite Parts

Composite Parts are parts made of basic parts that combine the functions of each basic part to provide a higher-order function, such as the production PETase.

The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon.

Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase.

Overall, there are 8 composite parts, which consist of the following:

BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon

BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon

BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon

BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon

BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon

BBa_K201005: T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon

BBa_K201007: T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon

BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon