Difference between revisions of "Team:Harvard BioDesign/Achievements"

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<h2><strong>Harvard BioDesign 2016</strong>.</h2>
 
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<h2>Achievements</h2>
 
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<h3>Project Achievements:</h3>
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<li>Constructed BioBricks for PETase and confirmed presence of protein using sfGFP</li>
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<li>Constructed BioBricks with secretion systems for PETase</li>
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<li>Validated PETase expression through SDS Page and Western blot</li>
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<li>Validated PETase enzymatic activity through pNPB substrate assay</li>
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<li>Characterized growth of three bacteria species in TPA minimal media</li>
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<li>Optimized PH for growth and PETase activity</li>
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<li>Demonstrated TPA signal can be detected by voltage increase in microbial fuel cell</li>
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<h3>MEDAL REQUIREMENTS</h3>
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<h3>BRONZE</h3>
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<p>1.    REGISTER AND ATTEND [Completed]</p>
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<p>2.    DELIVERABLES [Completed] <br>
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We met all of the deliverable requirements: team wiki, poster, presentation, project attribution, registry parts page, sample submission, safety forms, and judging forms.</p>
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<p>3.  <a href="https://2016.igem.org/Team:Harvard_BioDesign/Attributions">ATTRIBUTIONS </a> [Completed]</p>
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<p>4.    PART/ CONTRIBUTION [Completed]<br>
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<We documented and submitted to the registry BioBrick part <a href = “part.igem.org/Part:Bba_K2010000”> Bba_K2010000 (PETase) </a> </p>
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<p>The Harvard iGEM team began our brainstorming sessions during the late spring. We had a massive brainstorming session where we generated topics to investigate, and in the following weeks, scoured the internet for previous research relating to these topics. We met a few more times to discuss what we had found. After narrowing our search down to 4 projects, we wrote mini proposals to present each idea to the rest of the team. At the beginning of the summer, we continued our project planning with one project: Breaking PET. </p>
 
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<p>In Breaking PET, we hope to engineer a biological system that breaks down PET, a molecule which makes up one sixth of plastic products. Our project is grounded in research done by the Oda group at Kyoto Institute of Technology. In the paper “A bacteria that degrades and assimilates poly(ethylene terephthalate)”, they describe a bacteria found at a bottle recycling plant that has the ability to metabolize PET. From this paper, we found enzyme sequences for the two enzymes thought responsible for PET degradation: PETase and MHETase. PETase breaks down PET into the compound MHET, or mono(2-hydroxylethyl)terephthalic acid. MHETase further breaks down MHET into terephthalic acid and ethylene glycol. The discovery of these two enzymes is exciting because, unlike other PET degrading enzymes, PETase and MHETase are dedicated to the role of PET degradation. Additionally, PETase has been shown to have a degrading efficiency 120 times greater than alternative enzymes.</p>
 
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<p>Our project aims to use E Coli transformed with PETase and MHETase to break down PET and generate electricity. We will create BioBricks for PETase and MHETase and characterize these parts against enzymes used in previous PET degrading iGEM projects. Additionally, we hope to engineer a secretion system for these enzymes. We will also attempt to make a bioreactor/microbial fuel cell to generate electricity from the products of the PET degradation process.</p>
 
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<h3>SILVER</h3>
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<p>1.    VALIDATED PART/ VALIDATED CONTRIBUTION [Completed]
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<br><a href= “part.igem.org/Part:BBa_K2010007” BBa_K2010007> (pelB + sfGFP + PETase; PET-degrading enzyme, origin I. sakaiensis) </a> </p>
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<p>2.    COLLABORATION [Completed]
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<br><a href= “https://2016.igem.org/Team:Harvard_BioDesign/Collaborations”> We collaborated with team Northeastern by characterizing each other’s constructs. </a></p>
  
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<p>3.    HUMAN PRACTICES
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<br>Our team has identified, investigated, and addressed issues relating to safety, sustainability, and education. <a href= “https://2016.igem.org/Team:Harvard_BioDesign/Human_Practices”> Read about it here! </a> </p>
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<h3>GOLD</h3>
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<p>1.    INTEGRATED HUMAN PRACTICES [Completed]
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<br>The design and direction of our project were directly influenced by the feedback we received from both The Seabin Project and microbial fuel cell experts we interviewed throughout the duration of our project. (For example, The Seabin Project brought to our attention real-life factors typically unaccounted for in a lab setting, such as the variance in ocean plastic age; from there, we obtained plastic collected from a beach cleanup for experimental use. Moreover, after interviewing Professor Peter Girguis, Ph.D. we modified the design of our project, particularly the usage of the electrical output of our microbial fuel cell.) <a href= “https://2016.igem.org/Team:Harvard_BioDesign/HP/Gold”> Read more about it here! </a> </p>
  
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<p>2.   IMPROVE A PREVIOUS PART OR PROJECT [Completed]
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<br>We further characterized Team UC Davis’ (2012) part <a href= “http://partsregistry.org/wiki/index.php?title=Part:BBa_K936020”>  
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<p>3. <a href= “https://2016.igem.org/Team:Harvard_BioDesign/Proof”> PROOF OF CONCEPT </a> [Completed] </p>
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Latest revision as of 12:54, 19 January 2017

Harvard BioDesign 2016

Achievements

Project Achievements:

  • Constructed BioBricks for PETase and confirmed presence of protein using sfGFP
  • Constructed BioBricks with secretion systems for PETase
  • Validated PETase expression through SDS Page and Western blot
  • Validated PETase enzymatic activity through pNPB substrate assay
  • Characterized growth of three bacteria species in TPA minimal media
  • Optimized PH for growth and PETase activity
  • Demonstrated TPA signal can be detected by voltage increase in microbial fuel cell


MEDAL REQUIREMENTS

BRONZE

1. REGISTER AND ATTEND [Completed]

2. DELIVERABLES [Completed]
We met all of the deliverable requirements: team wiki, poster, presentation, project attribution, registry parts page, sample submission, safety forms, and judging forms.

3. ATTRIBUTIONS [Completed]

4. PART/ CONTRIBUTION [Completed]
Bba_K2010000 (PETase)

SILVER

1. VALIDATED PART/ VALIDATED CONTRIBUTION [Completed]
(pelB + sfGFP + PETase; PET-degrading enzyme, origin I. sakaiensis)

2. COLLABORATION [Completed]
We collaborated with team Northeastern by characterizing each other’s constructs.

3. HUMAN PRACTICES
Our team has identified, investigated, and addressed issues relating to safety, sustainability, and education. Read about it here!

GOLD

1. INTEGRATED HUMAN PRACTICES [Completed]
The design and direction of our project were directly influenced by the feedback we received from both The Seabin Project and microbial fuel cell experts we interviewed throughout the duration of our project. (For example, The Seabin Project brought to our attention real-life factors typically unaccounted for in a lab setting, such as the variance in ocean plastic age; from there, we obtained plastic collected from a beach cleanup for experimental use. Moreover, after interviewing Professor Peter Girguis, Ph.D. we modified the design of our project, particularly the usage of the electrical output of our microbial fuel cell.) Read more about it here!

2. IMPROVE A PREVIOUS PART OR PROJECT [Completed]
We further characterized Team UC Davis’ (2012) part BBa_K936000

3. PROOF OF CONCEPT [Completed]