Difference between revisions of "Team:BostonU/Proof"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">gold medal criterion for proof of concept</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<p style = "text-align:center; font-size:200%; padding:5px 0px 0px 0px;">Phase 1</p>
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<div class = "thebuttons" id = "parenttwo" style="margin:0px 150px 0px 150px;">
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<p style = "text-align:center; font-size:200%; padding:5px 0px 0px 0px;">Phase 2</p>
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<p style = "text-align:center; font-size:200%; padding:5px 0px 0px 0px;">Phase 3</p>
 
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<p style = "font-size:260%; color:#0071A7; text-align:center;">Phase 1</p>
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<p style = "text-indent:70px; font-size:150%; padding:25px 150px 50px 150px; color:#0071A7;">The goal of phase 1 to establish a consistent method of gene activation through dCAS9-VPR. Using MIT's CRISPR  optimization tool, we generated 20 sequences to be used as our gRNA and target site. The dCAS9-VPR complex, the target sequence and reporter gene, and the gRNA expression vectors were constructed and transfected into HEK293 cells. Another set of transfections took place simultaneously with the same materials minus the gRNA expression vector as a negative control. The fold increase between the basal level of expression from the control and the activated level of expression was then recorded. The results can be found below.</p>
  
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<center><img src = "https://static.igem.org/mediawiki/2016/a/aa/T--BostonU--InitialData.jpg"></center>
  
  
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iGEM teams are great at making things work! We value teams not only doing an incredible job with theoretical models and experiments, but also in taking the first steps to make their project real.
 
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</p>
 
  
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<p style = "text-indent:70px; font-size:150%; padding:25px 150px 50px 150px; color:#0071A7;">The goal of phase 1 to establish a consistent method of gene activation through dCAS9-VPR. Using MIT's CRISPR  optimization tool, we generated 20 sequences to be used as our gRNA and target site. The dCAS9-VPR complex, the target sequence and reporter gene, and the gRNA expression vectors were constructed and transfected into HEK293 cells. Another set of transfections took place simultaneously with the same materials minus the gRNA expression vector as a negative control. The fold increase between the basal level of expression from the control and the activated level of expression was then recorded. The results can be found below. Here are some extra words to make it seem like this is a different paragraph than the rest when really, it's not. It's just the same thing. But I want to make it look different, so there you go. Let's just add a few more words, and there we go. That should do it.</p>
  
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<h4> What should we do for our proof of concept? </h4>
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<center><img style = "width:1000px;" src = "https://static.igem.org/mediawiki/2016/3/3f/T--BostonU--ThreeGenes.jpg"></center>
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<br>
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You can assemble a device from BioBricks and show it works. You could build some equipment if you're competing for the hardware award. You can create a working model of your software for the software award. Please note that this not an exhaustive list of activities you can do to fulfill the gold medal criterion. As always, your aim is to impress the judges!
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<p style = "text-indent:70px; font-size:150%; padding:25px 150px 50px 150px; color:#0071A7;">The goal of phase 1 to establish a consistent method of gene activation through dCAS9-VPR. Using MIT's CRISPR  optimization tool, we generated 20 sequences to be used as our gRNA and target site. The dCAS9-VPR complex, the target sequence and reporter gene, and the gRNA expression vectors were constructed and transfected into HEK293 cells. Another set of transfections took place simultaneously with the same materials minus the gRNA expression vector as a negative control. The fold increase between the basal level of expression from the control and the activated level of expression was then recorded. The results can be found below.</p>
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<center><img src = "https://static.igem.org/mediawiki/2016/f/f6/T--BostonU--Orthogonality.jpg" style = "width:700px;"></center>
  
 
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<p style = "font-size:260%; color:#0071A7; text-align:center;">Phase 2</p>
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<center><hr style= "width:550px; height: 4px; background-color:#0071A7"></center><br>
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<p style = "text-indent:70px; font-size:150%; padding:25px 150px 50px 150px; color:#0071A7;">Words will go here that explain all the experiments we conducted to achieve aim one. I don't know what those words will be yet, but hey, I'm just the html guy not the writer guy. I mean, I do enjoy writing, but right now I have other things to work on. I just have to remember to come back and change this part. If I forget, that would bad, but I don't think I will. Here are some extra words to make it seem like this is a different pargraph than the rest when really, it's not. It's just the same thing. But I want to make it look different, so there you go. Let's just add a few more words, and there we go. That should do it. Shoot, this still isn't  enough words. Hold on, here a few more, and a few more. Okay, now I'm out of ideas.</p>
  
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<p style = "font-size:260%; color:#0071A7; text-align:center;">Phase 3</p>
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<center><hr style= "width:550px; height: 4px; background-color:#0071A7"></center><br>
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<p style = "text-indent:70px; font-size:150%; padding:25px 150px 50px 150px; color:#0071A7;">These words have to be a little different, just to show that the words are changing. Everything else from here on in will be identical to phase  though. So without further ado, here's phase 2 again. Words will go here that explain all the experiments we conducted to achieve aim one. I don't know what those words will be yet, but hey, I'm just the html guy not the writer guy. I mean, I do enjoy writing, but right now I have other things to work on. I just have to remember to come back and change this part. If I forget, that would bad, but I don't think I will. Here are some extra words to make it seem like this is a different pargraph than the rest when really, it's not. It's just the same thing. But I want to make it look different, so there you go.</p>
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Revision as of 15:53, 9 August 2016





Phase 1

Phase 2

Phase 3




Phase 1


The goal of phase 1 to establish a consistent method of gene activation through dCAS9-VPR. Using MIT's CRISPR optimization tool, we generated 20 sequences to be used as our gRNA and target site. The dCAS9-VPR complex, the target sequence and reporter gene, and the gRNA expression vectors were constructed and transfected into HEK293 cells. Another set of transfections took place simultaneously with the same materials minus the gRNA expression vector as a negative control. The fold increase between the basal level of expression from the control and the activated level of expression was then recorded. The results can be found below.


The goal of phase 1 to establish a consistent method of gene activation through dCAS9-VPR. Using MIT's CRISPR optimization tool, we generated 20 sequences to be used as our gRNA and target site. The dCAS9-VPR complex, the target sequence and reporter gene, and the gRNA expression vectors were constructed and transfected into HEK293 cells. Another set of transfections took place simultaneously with the same materials minus the gRNA expression vector as a negative control. The fold increase between the basal level of expression from the control and the activated level of expression was then recorded. The results can be found below. Here are some extra words to make it seem like this is a different paragraph than the rest when really, it's not. It's just the same thing. But I want to make it look different, so there you go. Let's just add a few more words, and there we go. That should do it.


The goal of phase 1 to establish a consistent method of gene activation through dCAS9-VPR. Using MIT's CRISPR optimization tool, we generated 20 sequences to be used as our gRNA and target site. The dCAS9-VPR complex, the target sequence and reporter gene, and the gRNA expression vectors were constructed and transfected into HEK293 cells. Another set of transfections took place simultaneously with the same materials minus the gRNA expression vector as a negative control. The fold increase between the basal level of expression from the control and the activated level of expression was then recorded. The results can be found below.


Phase 2


Words will go here that explain all the experiments we conducted to achieve aim one. I don't know what those words will be yet, but hey, I'm just the html guy not the writer guy. I mean, I do enjoy writing, but right now I have other things to work on. I just have to remember to come back and change this part. If I forget, that would bad, but I don't think I will. Here are some extra words to make it seem like this is a different pargraph than the rest when really, it's not. It's just the same thing. But I want to make it look different, so there you go. Let's just add a few more words, and there we go. That should do it. Shoot, this still isn't enough words. Hold on, here a few more, and a few more. Okay, now I'm out of ideas.


Phase 3


These words have to be a little different, just to show that the words are changing. Everything else from here on in will be identical to phase though. So without further ado, here's phase 2 again. Words will go here that explain all the experiments we conducted to achieve aim one. I don't know what those words will be yet, but hey, I'm just the html guy not the writer guy. I mean, I do enjoy writing, but right now I have other things to work on. I just have to remember to come back and change this part. If I forget, that would bad, but I don't think I will. Here are some extra words to make it seem like this is a different pargraph than the rest when really, it's not. It's just the same thing. But I want to make it look different, so there you go.