Difference between revisions of "Team:Harvard BioDesign/Safety"

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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<h1><a href="https://2016.igem.org/Team:Harvard_BioDesign" id="logo">Harvard BioDesign</a></h1>
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<hr />
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<p>Description</p>
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</header>
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<footer>
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<a href="#banner" class="button circled scrolly">Start</a>
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</footer>
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</div>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
  
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<nav id="nav" >
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<ul>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign">Home</a></li>
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<li>
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<a href="#">Team</a>
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<ul>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Team">Team</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Collaborations">Collaborations</a></li>
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</ul>
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</li>
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<li>
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<a href="#">Project</a>
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<ul>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Description">Description</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Design">Design</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Experiments">Experiments</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Proof">Proof of Concept</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Demonstrate">Demonstrate</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Results">Results</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Notebook">Notebook</a></li>
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</ul>
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</li>
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<li>
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<a href="#">Parts</a>
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<ul>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Parts">Parts</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Basic_Part">Basic Parts</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Composite_Part">Composite Parts</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Part_Collection">Part Collection</a></li>
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</ul>
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</li>
  
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<!-- R edits -->
  
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<div class="column full_size">
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Safety">Safety</a></li>
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<h5>Safe Project Design</h5>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Attributions">Attributions</a></li>
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<li>
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<a href="#">Human Practices</a>
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<ul>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Human_Practices">Human Practices</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/HP/Silver">Silver</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/HP/Gold">Gold</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Integrated_Practices">Integrated Practices</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Engagement">Engagement</a></li>
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</ul>
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</li>
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<li>
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<a href="#">Awards</a>
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<ul>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Model">Model</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Achievements">Achievements</a></li>
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</ul>
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</li>
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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</ul>
 +
</nav>
  
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<ul>
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</div>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<div class="wrapper style1">
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<div class="container">
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<article id="main" class="special">
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<header>
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<h2><a href="#">Safety</a></h2>
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<p>
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</p>
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</header>
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<a href="#" class="image featured"><img src="images/bom01.jpg" alt="" /></a>
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<p>
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</p>
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<section><p>Considering the implications of synthetic biology tools to the world beyond the lab, It is important to maintain safe and ethical use of engineered organisms. </p></section>
 +
 
 +
 
 +
<section>
 +
<header><h3>Wet Lab Safety</h3><header>
 +
<p>Even a relatively innocuous chassis such as E. coli can be risky to use if improperly handled. To prevent exposure, we wore basic personal protective equipment in the lab at all times. This included appropriate clothing, covered shoes, lab coats, goggles, and disposable gloves.</p>
 +
 +
 
 +
<p>When choosing bacteria to use in our microbial fuel cell for their special ability to degrade Terephthalic Acid, a by-product of PET plastic degradation, we were careful to select only BSL1 organisms.</p>
 +
 
 +
<p>To prevent our engineered microbes from being released into the environment, we followed our host lab’s guidelines for proper disposal of organic waste.</p>
 +
</section>
 +
 
 +
<section><header><h3>General Lab Safety</h3></header>
 +
<p>Some techniques involved using chemicals that are hazardous to inhale. When working with such chemicals, we were careful to only work under the fume hood. Flammables were stored in a separate cabinet and disposed of according to strict guidelines. </p>
 +
</section>
 +
 
 +
<section>
 +
<header><h3>Safety in Collaboration</h3></header>
 +
<p>We transported materials between our lab and the Northeastern team’s lab in sealed containment to prevent release. </p>
 +
</section>
 +
 
 +
<section>
 +
<header><h3>Safety in Design</h3></header>
 +
 
 +
<p>Since we were working on a prototype with possible real-life applications, we considered safety when making crucial decisions about important aspects of our design.
 +
iGEM suggests that teams consider the following questions when designing their projects:</p>
 +
<ul><h4>Who will use your product? What opinions do these people have about your project?</h4>
 +
<li>Our product is intended to be used as a plastic-mapping device for parties concerned with the distribution of plastic in the ocean. Organisations that we reached out to when considering our design (need notes from outreach)</li>
 
</ul>
 
</ul>
  
−
</div>
+
<ul><h4>Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean?</h4>
 +
<li>Because our prototype is envisioned to be used in the ocean, we have come up with a bioreactor design that ensures the engineered microbes act as a closed system that is not released into the ocean environment.</li>
 +
</ul>
 +
 
 +
<ul><h4>If your product is successful, who will receive benefits and who will be harmed?</h4>
 +
<li>Direct benefits of a successful and efficient plastic-sensing system, such as Plastiback will be felt by researchers looking to map the distribution of plastics in the ocean. This could really affect how well the effects of plastic pollution are neutralized. The device promises benefits to the greater public through improvement of our environmental systems.</li>
 +
<li>As part of the prototype stage of our design, we are considering the environmental impact of the product in the oceans. Future directions include design solutions to the following important questions:
 +
<ul>How do we prevent small animals from getting trapped in the device?</ul>
 +
<ul>Will the device stay afloat and be easy to retrieve from the ocean?</ul>
 +
</li>
 +
</ul>
 +
 
 +
<ul><h4>What happens when it's all used up? Will it be sterilized, discarded, or recycled?</h4>
 +
<li>At the end of the object’s life cycle, it will  be retrieved, sterilized, and reused. Collected debris will go to appropriate waste management systems.</li>
 +
</ul>
 +
 +
<ul><h4>Is it safer, cheaper, or better than other technologies that do the same thing?</h4>
 +
<li>Sources:
 +
<ul><a href="http://news.nationalgeographic.com/news/2014/07/140715-ocean-plastic-debris-trash-pacific-garbage-patch/">http://news.nationalgeographic.com/news/2014/07/140715-ocean-plastic-debris-trash-pacific-garbage-patch/</a></ul>
 +
<ul></ul>
 +
<ul></ul>
 +
<ul></ul>
 +
<ul></ul>
 +
 
 +
</li>
 +
 
 +
<li>Conversations to have:
 +
<ul>Patrick Ulrich (pulrich@seas.harvard.edu)</ul>
 +
<ul>5gyres: http://www.5gyres.org/publications/</ul>
 +
<ul>http://news.harvard.edu/gazette/story/2012/02/trouble-afloat-ocean-plastics</ul>
 +
<ul></ul>
 +
</li>
 +
</ul>
 +
 
 +
 
 +
Who will use your product? What opinions do these people have about your project?
 +
Our product is intended to be used as a plastic-mapping device for parties concerned with the distribution of plastic in the ocean. Organisations that we reached out to when considering our design (need notes from outreach)
 +
Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean?
 +
Because our prototype is envisioned to be used in the ocean, we have come up with a bioreactor design that ensures the engineered microbes act as a closed system that is not released into the ocean environment.
 +
If your product is successful, who will receive benefits and who will be harmed?
 +
Direct benefits of a successful and efficient plastic-sensing system, such as Plastiback will be felt by researchers looking to map the distribution of plastics in the ocean. This could really affect how well the effects of plastic pollution are neutralized. The device promises benefits to the greater public through improvement of our environmental systems.
 +
As part of the prototype stage of our design, we are considering the environmental impact of the product in the oceans. Future directions include design solutions to the following important questions:
 +
How do we prevent small animals from getting trapped in the device?
 +
Will the device stay afloat and be easy to retrieve from the ocean?
 +
What happens when it's all used up? Will it be sterilized, discarded, or recycled?
 +
At the end of the object’s life cycle, it will  be retrieved, sterilized, and reused. Collected debris will go to appropriate waste management systems.
 +
Is it safer, cheaper, or better than other technologies that do the same thing?
 +
Sources: http://news.nationalgeographic.com/news/2014/07/140715-ocean-plastic-debris-trash-pacific-garbage-patch/
 +
Conversations to have:
 +
Patrick Ulrich (pulrich@seas.harvard.edu)
 +
5gyres: http://www.5gyres.org/publications/
 +
http://news.harvard.edu/gazette/story/2012/02/trouble-afloat-ocean-plastics/
 +
 
 +
From Wiki Guidelines:
 +
 
 +
Safe Project Design
 +
Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:
 +
Choosing a non-pathogenic chassis
 +
Choosing parts that will not harm humans / animals / plants
 +
Substituting safer materials for dangerous materials in a proof-of-concept experiment
 +
Including an "induced lethality" or "kill-switch" device
 +
Safe Lab Work
 +
What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!
 +
Safe Shipment
 +
Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?
 +
 
 +
 
 +
</article>
 +
<hr />
 +
<div class="row">
 +
<article class="4u 12u(mobile) special">
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
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</div>
+
</ul>
 +
</section>
  
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+
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<h5>Safe Shipment</h5>
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<li>&copy; 2016 Harvard iGEM</li><li>Design: <a href="http://html5up.net">HTML5 UP</a></li>
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</ul>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Revision as of 14:56, 8 October 2016

Harvard BioDesign 2016

Safety

Morbi convallis lectus malesuada sed fermentum dolore amet

Commodo id natoque malesuada sollicitudin elit suscipit. Curae suspendisse mauris posuere accumsan massa posuere lacus convallis tellus interdum. Amet nullam fringilla nibh nulla convallis ut venenatis purus lobortis. Auctor etiam porttitor phasellus tempus cubilia ultrices tempor sagittis. Nisl fermentum consequat integer interdum integer purus sapien. Nibh eleifend nulla nascetur pharetra commodo mi augue interdum tellus. Ornare cursus augue feugiat sodales velit lorem. Semper elementum ullamcorper lacinia natoque aenean scelerisque vel lacinia mollis quam sodales congue.

Considering the implications of synthetic biology tools to the world beyond the lab, It is important to maintain safe and ethical use of engineered organisms.

Wet Lab Safety

Even a relatively innocuous chassis such as E. coli can be risky to use if improperly handled. To prevent exposure, we wore basic personal protective equipment in the lab at all times. This included appropriate clothing, covered shoes, lab coats, goggles, and disposable gloves.

When choosing bacteria to use in our microbial fuel cell for their special ability to degrade Terephthalic Acid, a by-product of PET plastic degradation, we were careful to select only BSL1 organisms.

To prevent our engineered microbes from being released into the environment, we followed our host lab’s guidelines for proper disposal of organic waste.

General Lab Safety

Some techniques involved using chemicals that are hazardous to inhale. When working with such chemicals, we were careful to only work under the fume hood. Flammables were stored in a separate cabinet and disposed of according to strict guidelines.

Safety in Collaboration

We transported materials between our lab and the Northeastern team’s lab in sealed containment to prevent release.

Safety in Design

Since we were working on a prototype with possible real-life applications, we considered safety when making crucial decisions about important aspects of our design. iGEM suggests that teams consider the following questions when designing their projects:

    Who will use your product? What opinions do these people have about your project?

  • Our product is intended to be used as a plastic-mapping device for parties concerned with the distribution of plastic in the ocean. Organisations that we reached out to when considering our design (need notes from outreach)

    Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean?

  • Because our prototype is envisioned to be used in the ocean, we have come up with a bioreactor design that ensures the engineered microbes act as a closed system that is not released into the ocean environment.

    If your product is successful, who will receive benefits and who will be harmed?

  • Direct benefits of a successful and efficient plastic-sensing system, such as Plastiback will be felt by researchers looking to map the distribution of plastics in the ocean. This could really affect how well the effects of plastic pollution are neutralized. The device promises benefits to the greater public through improvement of our environmental systems.
  • As part of the prototype stage of our design, we are considering the environmental impact of the product in the oceans. Future directions include design solutions to the following important questions:
      How do we prevent small animals from getting trapped in the device?
      Will the device stay afloat and be easy to retrieve from the ocean?

    What happens when it's all used up? Will it be sterilized, discarded, or recycled?

  • At the end of the object’s life cycle, it will be retrieved, sterilized, and reused. Collected debris will go to appropriate waste management systems.
Who will use your product? What opinions do these people have about your project? Our product is intended to be used as a plastic-mapping device for parties concerned with the distribution of plastic in the ocean. Organisations that we reached out to when considering our design (need notes from outreach) Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean? Because our prototype is envisioned to be used in the ocean, we have come up with a bioreactor design that ensures the engineered microbes act as a closed system that is not released into the ocean environment. If your product is successful, who will receive benefits and who will be harmed? Direct benefits of a successful and efficient plastic-sensing system, such as Plastiback will be felt by researchers looking to map the distribution of plastics in the ocean. This could really affect how well the effects of plastic pollution are neutralized. The device promises benefits to the greater public through improvement of our environmental systems. As part of the prototype stage of our design, we are considering the environmental impact of the product in the oceans. Future directions include design solutions to the following important questions: How do we prevent small animals from getting trapped in the device? Will the device stay afloat and be easy to retrieve from the ocean? What happens when it's all used up? Will it be sterilized, discarded, or recycled? At the end of the object’s life cycle, it will be retrieved, sterilized, and reused. Collected debris will go to appropriate waste management systems. Is it safer, cheaper, or better than other technologies that do the same thing? Sources: http://news.nationalgeographic.com/news/2014/07/140715-ocean-plastic-debris-trash-pacific-garbage-patch/ Conversations to have: Patrick Ulrich (pulrich@seas.harvard.edu) 5gyres: http://www.5gyres.org/publications/ http://news.harvard.edu/gazette/story/2012/02/trouble-afloat-ocean-plastics/ From Wiki Guidelines: Safe Project Design Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example: Choosing a non-pathogenic chassis Choosing parts that will not harm humans / animals / plants Substituting safer materials for dangerous materials in a proof-of-concept experiment Including an "induced lethality" or "kill-switch" device Safe Lab Work What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here! Safe Shipment Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?

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