Difference between revisions of "Team:Tianjin/Experiment/CFPS"
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<h1 id="about" class="title text-center">Experiment of <span>CFPS</span></h1> | <h1 id="about" class="title text-center">Experiment of <span>CFPS</span></h1> | ||
| − | + | <h2><b>Overview</b></h2> | |
| + | <p style="font-size:18px">The ability to produce a functional protein in the test tube, rather than in cells, is the essence of cell-free protein synthesis (CFPS)[1].</i> .</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/5/5e/T--Tianjin--cf-e-1.png" alt="desktop"> | ||
| + | <p style="font-size:15px"> | ||
| + | <br/> | ||
| + | Fig.1. The basic process of CFPS system | ||
| + | </p> | ||
| + | <p style="font-size:18px">The lack of high-throughput approaches for expression and screening of large enzyme libraries remains a | ||
| + | major bottleneck for current enzyme engineering efforts. To address this need, some researchers[2] have developed a high-throughput, fluorescence-based approach for rapid one-pot, microscale expression, and screening of | ||
| + | different kinds of enzymes. To go further, we try to make our effert to achieve integration of cell-free protein expression with activity screening of enzymes( site-directed mutations of <i>PETase</i>). | ||
| + | </i> .</p> | ||
<!-- section end --> | <!-- section end --> | ||
Revision as of 01:43, 5 October 2016
Experiment of CFPS
Overview
The ability to produce a functional protein in the test tube, rather than in cells, is the essence of cell-free protein synthesis (CFPS)[1]. .
Fig.1. The basic process of CFPS system
The lack of high-throughput approaches for expression and screening of large enzyme libraries remains a major bottleneck for current enzyme engineering efforts. To address this need, some researchers[2] have developed a high-throughput, fluorescence-based approach for rapid one-pot, microscale expression, and screening of different kinds of enzymes. To go further, we try to make our effert to achieve integration of cell-free protein expression with activity screening of enzymes( site-directed mutations of PETase). .
