Difference between revisions of "Team:BostonU/Composite Part"
| Line 14: | Line 14: | ||
<br><p style = "font-size:150%; padding:25px 150px 20px 150px; color:#0071A7;"> | <br><p style = "font-size:150%; padding:25px 150px 20px 150px; color:#0071A7;"> | ||
| − | BBa_K1875018</p> | + | <a href = "http://parts.igem.org/Part:BBa_K1875018" style = "color:blue;">BBa_K1875018</a></p> |
<br><p style = "font-size:150%; padding:25px 150px 20px 150px; color:#0071A7;"> | <br><p style = "font-size:150%; padding:25px 150px 20px 150px; color:#0071A7;"> | ||
To create the Gemini parts library we made use of two “classes” of plasmid: guide RNA expressing vectors and guide RNA operator reporter vectors. When these two plasmids are co-transfected with a dCas9-VPR plasmid into a mammalian cell line, the production of a gene of interest proceeds. The genes of interest in our work were fluorescent proteins; specifically GFP was utilized in the guide RNA operator reporter vectors submitted to the registry. The guide RNA operator vector can be considered a composite part. It is constructed by combining the following parts in order: BBa_K1875009 (the target sequence), BBa_K1875000 (the miniCMV), BBa_K1875001 (the Kozak sequence), BBa_K1875003 (the GFP), BBa_K1875002 (the Rabbit Beta Globin PolyA). The sources of these parts can be found on their respective registry pages. We have submitted BBa_K1875018 for best composite part due to both its high expression and consistency in experimentation. Specifically, this part, when compared with a CMV, can express GFP at a high level than the strong promoter. This means that this part grants a scientist the ability to produce a protein at a higher level than a strong promoter and grant them a temporal control. | To create the Gemini parts library we made use of two “classes” of plasmid: guide RNA expressing vectors and guide RNA operator reporter vectors. When these two plasmids are co-transfected with a dCas9-VPR plasmid into a mammalian cell line, the production of a gene of interest proceeds. The genes of interest in our work were fluorescent proteins; specifically GFP was utilized in the guide RNA operator reporter vectors submitted to the registry. The guide RNA operator vector can be considered a composite part. It is constructed by combining the following parts in order: BBa_K1875009 (the target sequence), BBa_K1875000 (the miniCMV), BBa_K1875001 (the Kozak sequence), BBa_K1875003 (the GFP), BBa_K1875002 (the Rabbit Beta Globin PolyA). The sources of these parts can be found on their respective registry pages. We have submitted BBa_K1875018 for best composite part due to both its high expression and consistency in experimentation. Specifically, this part, when compared with a CMV, can express GFP at a high level than the strong promoter. This means that this part grants a scientist the ability to produce a protein at a higher level than a strong promoter and grant them a temporal control. | ||
Revision as of 17:16, 14 October 2016
To create the Gemini parts library we made use of two “classes” of plasmid: guide RNA expressing vectors and guide RNA operator reporter vectors. When these two plasmids are co-transfected with a dCas9-VPR plasmid into a mammalian cell line, the production of a gene of interest proceeds. The genes of interest in our work were fluorescent proteins; specifically GFP was utilized in the guide RNA operator reporter vectors submitted to the registry. The guide RNA operator vector can be considered a composite part. It is constructed by combining the following parts in order: BBa_K1875009 (the target sequence), BBa_K1875000 (the miniCMV), BBa_K1875001 (the Kozak sequence), BBa_K1875003 (the GFP), BBa_K1875002 (the Rabbit Beta Globin PolyA). The sources of these parts can be found on their respective registry pages. We have submitted BBa_K1875018 for best composite part due to both its high expression and consistency in experimentation. Specifically, this part, when compared with a CMV, can express GFP at a high level than the strong promoter. This means that this part grants a scientist the ability to produce a protein at a higher level than a strong promoter and grant them a temporal control.
