Difference between revisions of "Team:BostonU/Composite Part"

Line 19: Line 19:
  
 
</p>
 
</p>
 
+
<br><br><br><br>
  
 
</div>
 
</div>

Revision as of 18:30, 14 October 2016


BBa_K1875018


To create the Gemini parts library we made use of two “classes” of plasmid: guide RNA expressing vectors and guide RNA operator reporter vectors. When these two plasmids are co-transfected with a dCas9-VPR plasmid into a mammalian cell line, the production of a gene of interest proceeds. The genes of interest in our work were fluorescent proteins; specifically GFP was utilized in the guide RNA operator reporter vectors submitted to the registry. The guide RNA operator vector can be considered a composite part. It is constructed by combining the following parts in order: BBa_K1875009 (the target sequence), BBa_K1875000 (the miniCMV), BBa_K1875001 (the Kozak sequence), BBa_K1875003 (the GFP), BBa_K1875002 (the Rabbit Beta Globin PolyA). The sources of these parts can be found on their respective registry pages. We have submitted BBa_K1875018 for best composite part due to both its high expression and consistency in experimentation. Specifically, this part, when compared with a CMV, can express GFP at a high level than the strong promoter. This means that this part grants a scientist the ability to produce a protein at a higher level than a strong promoter and grant them a temporal control.