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Revision as of 02:47, 18 October 2016

Harvard BioDesign 2016

Composite Parts

The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon.

Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase.

Overall, there are 8 composite parts, which consist of the following:

    BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon
    BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon
    BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon
    BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon
    BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon
    BBa_K201005: T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon
    BBa_K201007: T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon
    BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon