Difference between revisions of "Team:Harvard BioDesign/Composite Part"

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<section>
 
<p>Overall, there are 8 composite parts, which consist of the following:</p>
 
<p>Overall, there are 8 composite parts, which consist of the following:</p>
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<p><a href="http://parts.igem.org/Part:BBa_K2010000"><b>BBa_K201000:</b> T7 Promoter + PETase + His-Tag + Stop Codon</a></p>
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<p>BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon</p>
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<p><a href="http://parts.igem.org/Part:BBa_K2010001"><b>BBa_K201001:</b> T7 Promoter + ompT + PETase + His-Tag + Stop Codon</a></p>
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<p>BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon</p>
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<p><a href="http://parts.igem.org/Part:BBa_K2010002"><b>BBa_K201002:</b> T7 Promoter + pelB + PETase + His-Tag + Stop Codon</a></p>
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<p>BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon</p>
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<p><a href="http://parts.igem.org/Part:BBa_K2010003"><b>BBa_K201003:</b> T7 Promoter + YebF + PETase + His-Tag + Stop Codon</a></p>
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<p>BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon</p>
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<p><a href="http://parts.igem.org/Part:BBa_K2010004"><b>BBa_K201004:</b> T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon</a></p>
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<p>BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon</p>
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<p><a href="http://parts.igem.org/Part:BBa_K2010005"><b>BBa_K201005:</b> T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon</a></p>
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<p>BBa_K201005: T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon</p>
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<p><a href="http://parts.igem.org/Part:BBa_K2010006"><b>BBa_K201007:</b> T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon</a></p>
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<p>BBa_K201007: T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon</p>
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<p><a href="http://parts.igem.org/Part:BBa_K2010007"><b>BBa_K201008:</b> T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon</a></p>
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<p>BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon</p>
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</b>
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</section>
 
</section>
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Revision as of 02:58, 18 October 2016

Harvard BioDesign 2016

Composite Parts

The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon.

Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase.

Overall, there are 8 composite parts, which consist of the following:

BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon

BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon

BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon

BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon

BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon

BBa_K201005: T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon

BBa_K201007: T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon

BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon