Difference between revisions of "Team:Harvard BioDesign"

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<title>Harvard BioDesign 2016</title>
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<h3>Project Description</h3>
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The Harvard iGEM team began our brainstorming sessions during the late spring. We had a massive brainstorming session where we generated topics to investigate, and in the following weeks, scoured the internet for previous research relating to these topics. We met a few more times to discuss what we had found. After narrowing our search down to 4 projects, we wrote mini proposals to present each idea to the rest of the team. At the beginning of the summer, we continued our project planning with one project: Breaking PET.
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In Breaking PET, we hope to engineer a biological system that breaks down PET, a molecule which makes up one sixth of plastic products. Our project is grounded in research done by the Oda group at Kyoto Institute of Technology. In the paper “A bacteria that degrades and assimilates poly(ethylene terephthalate)”, they describe a bacteria found at a bottle recycling plant that has the ability to metabolize PET. From this paper, we found enzyme sequences for the two enzymes thought responsible for PET degradation: PETase and MHETase. PETase breaks down PET into the compound MHET, or mono(2-hydroxylethyl)terephthalic acid. MHETase further breaks down MHET into terephthalic acid and ethylene glycol. The discovery of these two enzymes is exciting because, unlike other PET degrading enzymes, PETase and MHETase are dedicated to the role of PET degradation. Additionally, PETase has been shown to have a degrading efficiency 120 times greater than alternative enzymes. 
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Our project aims to use E Coli transformed with PETase and MHETase to break down PET and generate electricity. We will create BioBricks for PETase and MHETase and characterize these parts against enzymes used in previous PET degrading iGEM projects. Additionally, we hope to engineer a secretion system for these enzymes. We will also attempt to make a bioreactor/microbial fuel cell to generate electricity from the products of the PET degradation process.
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<h2> Welcome to iGEM 2016! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h1><a href="https://2016.igem.org/Team:Harvard_BioDesign" id="logo">Harvard BioDesign</a></h1>
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<p>Description</p>
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<a href="#banner" class="button circled scrolly">Start</a>
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<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
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<ul>
 
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
 
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
 
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign">Home</a></li>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<a href="#">Team</a>
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</div>
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<ul>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Team">Team</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Collaborations">Collaborations</a></li>
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</ul>
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</li>
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<li>
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<a href="#">Project</a>
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<ul>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Description">Description</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Design">Design</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Experiments">Experiments</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Proof">Proof of Concept</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Results">Results</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Notebook">Notebook</a></li>
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</ul>
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</li>
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<li>
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<a href="#">Parts</a>
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<ul>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Basic_Part">Basic Parts</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Composite_Part">Composite Parts</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Part_Collection">Part Collection</a></li>
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</ul>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Safety">Safety</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Attributions">Attributions</a></li>
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<a href="#">Human Practices</a>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Human_Practices">Human Practices</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/HP/Silver">Silver</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/HP/Gold">Gold</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Integrated_Practices">Integrated Practices</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Engagement">Engagement</a></li>
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</ul>
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</li>
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<li>
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<a href="#">Awards</a>
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<ul>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Model">Model</a></li>
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<li><a href="https://2016.igem.org/Team:Harvard_BioDesign/Achievements">Achievements</a></li>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Have lots of fun! </li>
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<a href="#" class="image featured"><img src="images/pic04.jpg" alt="" /></a>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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</header>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<p>Commodo id natoque malesuada sollicitudin elit suscipit magna.</p>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Revision as of 04:04, 6 October 2016

Harvard BioDesign 2016

Sed massa imperdiet magnis

Sociis aenean eu aenean mollis mollis facilisis primis ornare penatibus aenean. Cursus ac enim pulvinar curabitur morbi convallis. Lectus malesuada sed fermentum dolore amet.

Commodo id natoque malesuada sollicitudin elit suscipit. Curae suspendisse mauris posuere accumsan massa posuere lacus convallis tellus interdum. Amet nullam fringilla nibh nulla convallis ut venenatis purus sit arcu sociis. Nunc fermentum adipiscing tempor cursus nascetur adipiscing adipiscing. Primis aliquam mus lacinia lobortis phasellus suscipit. Fermentum lobortis non tristique ante proin sociis accumsan lobortis. Auctor etiam porttitor phasellus tempus cubilia ultrices tempor sagittis. Nisl fermentum consequat integer interdum integer purus sapien. Nibh eleifend nulla nascetur pharetra commodo mi augue interdum tellus. Ornare cursus augue feugiat sodales velit lorem. Semper elementum ullamcorper lacinia natoque aenean scelerisque.

Morbi ullamcorper et varius leo lacus

Ipsum volutpat consectetur orci metus consequat imperdiet duis integer semper magna.

Gravida aliquam penatibus

Amet nullam fringilla nibh nulla convallis tique ante proin sociis accumsan lobortis. Auctor etiam porttitor phasellus tempus cubilia ultrices tempor sagittis. Nisl fermentum consequat integer interdum.

Sed quis rhoncus placerat

Amet nullam fringilla nibh nulla convallis tique ante proin sociis accumsan lobortis. Auctor etiam porttitor phasellus tempus cubilia ultrices tempor sagittis. Nisl fermentum consequat integer interdum.

Magna laoreet et aliquam

Amet nullam fringilla nibh nulla convallis tique ante proin sociis accumsan lobortis. Auctor etiam porttitor phasellus tempus cubilia ultrices tempor sagittis. Nisl fermentum consequat integer interdum.