Difference between revisions of "Team:GDSYZX-United/Proof"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">gold medal criterion for proof of concept</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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iGEM teams are great at making things work! We value teams not only doing an incredible job with theoretical models and experiments, but also in taking the first steps to make their project real.  
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<h4> What should we do for our proof of concept? </h4>
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You can assemble a device from BioBricks and show it works. You could build some equipment if you're competing for the hardware award. You can create a working model of your software for the software award. Please note that this not an exhaustive list of activities you can do to fulfill the gold medal criterion. As always, your aim is to impress the judges!
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<li><a href="https://2016.igem.org/Team:GDSYZX-United/Description">Project description</a></li>
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<li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/extract _dna">Extract DNA in arabidopsis thaliana</a></li>
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<h1><strong>Proof of concept</strong></h1>
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<h2><strong>Expression level of protein HHL1</strong></h2>
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<p><div class="text_font">After tranfection, we incubate the protoplasts at 2 groups of light intensities to induce the expression of hhl1.</div></p>
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<p><div class="text_font">We use semi-quantitive RT-PCR to amplify genehhl1 from mRNA in protoplasts，the concentration of amplified gene hhl1 of each group is measured by ultraviolet spectrophotometer. Data of the concentration of gene hhl1 is used to calculate the relative expression level of mRNA.</div></p>
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<table class= "table">
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<th></th>
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<th colspan="2">Growth light/th>
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<th colspan="2">High light</th>
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<td></td>
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<td>REL</td>
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<td>SE</td>
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<td>REL</td>
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<td>SE</td>
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<td>Phhl1</td>
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<td>1</td>
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<td>0.05</td>
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<td>1.1</td>
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<td>0.07</td>
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<td>Pcop1</td>
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<td>1</td>
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<td>0.03</td>
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<td>10.1</td>
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<td>1.26</td>
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<td>Ppif1</td>
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<td>1</td>
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<td>0.06</td>
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<td>8.9</td>
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<td>1.01</td>
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<td>PphyB</td>
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<td>1</td>
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<td>0.09</td>
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<td>15.6</td>
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<td>1.36</td>
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<p><div class="text_font">This graph shows the relative expression level of mRNA of gene hhl1 expressed by 4 different parts in growth light(100lux) and high light(1200lux).</div></p>
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<p><div class="text_font">pCop1,pPif1,pPhyB do not respond to growth light, the expression level of mRNA is similar to that of pHhl1.However, under high light,three photosensitive promoters all respond specifically and the expression level of mRNA is obviously higher than that in pHhl1.(The transcription level of pCop1,pPif1 and pPhyB under highlight are 10.1,8.9 and 15.6 times of the mRNA level under growth light,respectively.),suggesting that our parts can help produce more protein HHL1 to protect PSⅡ under high light.</div></p>
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<p><div class="text_font">(the relative expression level of mRNA of pHhl1 under high light doesn’t change much,but after translation the expression level of protein HHL1 is increased compare to pHhl1 under growth light).</div></p>
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Revision as of 17:22, 13 October 2016

Proof of concept

Expression level of protein HHL1

After tranfection, we incubate the protoplasts at 2 groups of light intensities to induce the expression of hhl1.

We use semi-quantitive RT-PCR to amplify genehhl1 from mRNA in protoplasts，the concentration of amplified gene hhl1 of each group is measured by ultraviolet spectrophotometer. Data of the concentration of gene hhl1 is used to calculate the relative expression level of mRNA.

image
Growth light/th> High light
REL SE REL SE
Phhl1 1 0.05 1.1 0.07
Pcop1 1 0.03 10.1 1.26
Ppif1 1 0.06 8.9 1.01
PphyB 1 0.09 15.6 1.36

This graph shows the relative expression level of mRNA of gene hhl1 expressed by 4 different parts in growth light(100lux) and high light(1200lux).

pCop1,pPif1,pPhyB do not respond to growth light, the expression level of mRNA is similar to that of pHhl1.However, under high light,three photosensitive promoters all respond specifically and the expression level of mRNA is obviously higher than that in pHhl1.(The transcription level of pCop1,pPif1 and pPhyB under highlight are 10.1,8.9 and 15.6 times of the mRNA level under growth light,respectively.),suggesting that our parts can help produce more protein HHL1 to protect PSⅡ under high light.

(the relative expression level of mRNA of pHhl1 under high light doesn’t change much,but after translation the expression level of protein HHL1 is increased compare to pHhl1 under growth light).