Difference between revisions of "Team:Harvard BioDesign/Composite Part"

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<h1><a href="https://2016.igem.org/Team:Harvard_BioDesign" id="logo">Harvard BioDesign</a></h1>
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<h2><a href="#">Composite Parts</a></h2>
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<p>
  
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<p>
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The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon.
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase.
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</p>
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Overall, there are 8 composite parts involved in Plastiback.
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</p>
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<section>
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<header>
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<h3>List of Composite Parts/h3>
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<p>
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BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon
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BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon
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BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon
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BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon
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BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon
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BBa_K201005: T7 Promoter + ompT + sfGFP + PETase + His-Tag + Stop Codon
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BBa_K201007: T7 Promoter + pelB + sfGFP + PETase + His-Tag + Stop Codon
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BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon
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</p>
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</section>
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</article>
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<hr />
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</div>
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</div>
  
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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Revision as of 20:18, 13 October 2016

Harvard BioDesign 2016

Composite Parts

The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon. Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase. Overall, there are 8 composite parts involved in Plastiback.

List of Composite Parts/h3>

BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon BBa_K201005: T7 Promoter + ompT + sfGFP + PETase + His-Tag + Stop Codon BBa_K201007: T7 Promoter + pelB + sfGFP + PETase + His-Tag + Stop Codon BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon