Difference between revisions of "GDSYZX-United/PROJECT/enzyme cleavage"
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Revision as of 04:06, 14 October 2016
Enzyme cleavage
Place:Biology Experiment Teaching Center Of Sun Yat-sen University
Date:July 27th, 2016
Experimental drugs: PCR products, Pst1restriction enzyme,10×buffer solution, ddH2O
Procedure:
-
enzyme cleavage:configuration
Blending, keeping in 37℃ for 1.5-3hPCR products (DNA) 5 μl 10×buffer solution 10 μl Pst1restriction enzyme 0.8μl ddH2O 84.2 μl -
electrophoresis
Take out 5μ DNA after enzyme cleavage to electrophoresis.
