Difference between revisions of "Team:BostonU/Part Collection"

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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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<br><p>The BostonU 2016 iGEM team created Gemini, a design space that combines digital and analog expression systems to easily modulate exogenous gene expression levels in human cells. The system relies on three components: a genome-orthogonal gRNA recognizes a corresponding DNA operator sequence upstream of a minimal promoter, and it recruits dCas9-VPR to transactivate the output gene. We developed a set of mutually-orthogonal gRNAs to enable multiplexed gene regulation without cross-talk, and a set of gRNA-operator plasmids to achieve varied expression levels. Our submitted parts collection has 2 gRNA expression devices (BBa_K1875011-BBa_K1875012) and 7 gRNA-operator devices (BBa_K1875013-BBa_K1875019). We validated these parts using flow cytometry. We demonstrated “digital” expression when comparing output gene activation with or without gRNAs, and “analog” expression when comparing different gRNA-operator architectures (single, multimerized, and mutated sequences). </p>
  
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.
 
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<h4>Note</h4>
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The team created pages for parts BBa_K1875000 - BBa_K1875019 and submitted parts BBa_K1875011 - BBa_K1875019.</p>
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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Revision as of 16:07, 14 October 2016


The BostonU 2016 iGEM team created Gemini, a design space that combines digital and analog expression systems to easily modulate exogenous gene expression levels in human cells. The system relies on three components: a genome-orthogonal gRNA recognizes a corresponding DNA operator sequence upstream of a minimal promoter, and it recruits dCas9-VPR to transactivate the output gene. We developed a set of mutually-orthogonal gRNAs to enable multiplexed gene regulation without cross-talk, and a set of gRNA-operator plasmids to achieve varied expression levels. Our submitted parts collection has 2 gRNA expression devices (BBa_K1875011-BBa_K1875012) and 7 gRNA-operator devices (BBa_K1875013-BBa_K1875019). We validated these parts using flow cytometry. We demonstrated “digital” expression when comparing output gene activation with or without gRNAs, and “analog” expression when comparing different gRNA-operator architectures (single, multimerized, and mutated sequences).


The team created pages for parts BBa_K1875000 - BBa_K1875019 and submitted parts BBa_K1875011 - BBa_K1875019.