Difference between revisions of "Team:Uppsala/Project/UnaG"
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| + | As part of this iGEM project, research has been done on the newly discovered green fluorescent protein called UnaG. This protein is interesting firstly because it is about half the size of other fluorescent proteins that are being used today, making it suitable as a fusion protein. Secondly, it fluoresces only when a bilirubin molecule is bound non-covalently to the protein. This feature could make UnaG suitable as an inducible marker by the addition and removal of bilirubin to cells. Moreover, unlike all other fluorescent proteins of today, UnaG does not need molecular oxygen in order to fluoresce, meaning that it can be used as a marker protein in research under hypoxic conditions. These cool features, alongside the fact that it is always exciting to work with new discoveries, made us want to add this protein to the iGEM toolbox! During the summer we created three different UnaG biobricks; one with a hexahistidine tag suitable for affinity chromatography, one with the hexahistidine tag and a flexible linker of six amino acids suitable for fusing to other proteins, and lastly one biobrick with only the flexible linker attached to UnaG. | ||
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Revision as of 11:14, 15 October 2016
UnaG
Stuff about Unag
Go Green - Go UnaG!
As part of this iGEM project, research has been done on the newly discovered green fluorescent protein called UnaG. This protein is interesting firstly because it is about half the size of other fluorescent proteins that are being used today, making it suitable as a fusion protein. Secondly, it fluoresces only when a bilirubin molecule is bound non-covalently to the protein. This feature could make UnaG suitable as an inducible marker by the addition and removal of bilirubin to cells. Moreover, unlike all other fluorescent proteins of today, UnaG does not need molecular oxygen in order to fluoresce, meaning that it can be used as a marker protein in research under hypoxic conditions. These cool features, alongside the fact that it is always exciting to work with new discoveries, made us want to add this protein to the iGEM toolbox! During the summer we created three different UnaG biobricks; one with a hexahistidine tag suitable for affinity chromatography, one with the hexahistidine tag and a flexible linker of six amino acids suitable for fusing to other proteins, and lastly one biobrick with only the flexible linker attached to UnaG.
UnaG
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Morbi commodo mollis mauris et euismod. Nullam pulvinar ultricies massa ac sagittis. Sed ex odio, venenatis non nisi sit amet, vulputate varius quam. Donec iaculis, orci a malesuada tempor, eros ex aliquam sem, eu laoreet odio erat at leo. Donec felis risus, aliquam eget blandit in, dignissim et arcu. Etiam id lectus sed augue ultrices condimentum. Donec bibendum eget leo tempus porttitor. Curabitur hendrerit ligula quis molestie scelerisque. Nunc quis ex a est finibus aliquam ac ac risus. Nam finibus ligula sed dignissim volutpat. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Morbi egestas eros sit amet fringilla viverra. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Etiam tempus aliquet turpis vitae posuere. Nam ante turpis, suscipit et nibh id, posuere porttitor justo. Nam vulputate tempus nulla, vel sagittis felis lobortis at. In tempor elit sit amet pellentesque pharetra. Fusce congue erat non lobortis aliquam. Aliquam consectetur nunc eros, pellentesque consectetur libero fermentum a. Sed condimentum tellus sapien, id laoreet velit dapibus ac. Praesent vitae commodo eros, vel posuere mi.
Bilirubin
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Morbi commodo mollis mauris et euismod. Nullam pulvinar ultricies massa ac sagittis. Sed ex odio, venenatis non nisi sit amet, vulputate varius quam. Donec iaculis, orci a malesuada tempor, eros ex aliquam sem, eu laoreet odio erat at leo. Donec felis risus, aliquam eget blandit in, dignissim et arcu. Etiam id lectus sed augue ultrices condimentum. Donec bibendum eget leo tempus porttitor. Curabitur hendrerit ligula quis molestie scelerisque. Nunc quis ex a est finibus aliquam ac ac risus. Nam finibus ligula sed dignissim volutpat. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Morbi egestas eros sit amet fringilla viverra. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Etiam tempus aliquet turpis vitae posuere. Nam ante turpis, suscipit et nibh id, posuere porttitor justo. Nam vulputate tempus nulla, vel sagittis felis lobortis at. In tempor elit sit amet pellentesque pharetra. Fusce congue erat non lobortis aliquam. Aliquam consectetur nunc eros, pellentesque consectetur libero fermentum a. Sed condimentum tellus sapien, id laoreet velit dapibus ac. Praesent vitae commodo eros, vel posuere mi.
Results
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Morbi commodo mollis mauris et euismod. Nullam pulvinar ultricies massa ac sagittis. Sed ex odio, venenatis non nisi sit amet, vulputate varius quam. Donec iaculis, orci a malesuada tempor, eros ex aliquam sem, eu laoreet odio erat at leo. Donec felis risus, aliquam eget blandit in, dignissim et arcu. Etiam id lectus sed augue ultrices condimentum. Donec bibendum eget leo tempus porttitor. Curabitur hendrerit ligula quis molestie scelerisque. Nunc quis ex a est finibus aliquam ac ac risus. Nam finibus ligula sed dignissim volutpat. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Morbi egestas eros sit amet fringilla viverra. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Etiam tempus aliquet turpis vitae posuere. Nam ante turpis, suscipit et nibh id, posuere porttitor justo. Nam vulputate tempus nulla, vel sagittis felis lobortis at. In tempor elit sit amet pellentesque pharetra. Fusce congue erat non lobortis aliquam. Aliquam consectetur nunc eros, pellentesque consectetur libero fermentum a. Sed condimentum tellus sapien, id laoreet velit dapibus ac. Praesent vitae commodo eros, vel posuere mi.
