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                                <li><a href="https://2016.igem.org/Team:HUST-China/Part_Collection">Part Collection</a></li>
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            <h2 class="text-center">Part collection</h2>
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            <p style="color:#fff;margin-bottom:10px;text-align:center;">Following documented composite parts are respectively from three circuits this year:</p>
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            <div class="ref"></div>
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            <article>
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            <h2>Collection list</h2>
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                <p>Here is the list of our part collection:</p>
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                <table border="1">
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<tr>
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  <td>Part Number</td>
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  <td>Name</td>
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  <td>Description</td>
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</tr>
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<tr>
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  <td><a href="http://parts.igem.org/Part:BBa_K2036009"> BBa_K2036009 </a></td>
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  <td> pRM-GFP-LVAssrAtag </td>
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  <td> A control group of Cro and pRM interaction characterization</td>
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</tr>
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<tr>
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  <td><a href="http://parts.igem.org/Part:BBa_K2036010"> BBa_K2036010 </a></td> 
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  <td>Cro-TT-pRM-RBS-GFP-LVAssrAtag </td>
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  <td> To test Cro and pRM interaction intensity.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K2036011"> BBa_K2036011 </a></td>
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  <td> pRE-GFP-LVAssrAtag</td>
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  <td> A control group of CII and pRE interaction</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K2036013"> BBa_K2036013 </a></td>
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  <td> RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag</td>
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  <td> To characterize CII’s interaction with pRE.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K2036014"> BBa_K2036014 </a></td>
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  <td> RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag</td>
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  <td> To characterize CIII’s interaction with Ftsh.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K2036015"> BBa_K2036015 </a></td>
 +
  <td> RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag</td>
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  <td>Tandem of CII amplifies its function.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K2036016"> BBa_K2036016 </a></td>
 +
  <td> pR-GFP-LVAssrAtag</td>
 +
  <td>A control group of CI and pR interaction.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K2036017"> BBa_K2036017 </a></td>
 +
  <td> CI-TT-pR-RBS-GFP-LVAssrAtag</td>
 +
  <td>To characterize CI and pR interaction.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K2036027"> BBa_K2036027</a></td>       
 +
<td>pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag</td>
 +
  <td>To verify the function of the whole circuit with T7 and ptrp sensing the signals.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K2036028"> BBa_K2036028</a></td>       
 +
<td>placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase</td>
 +
  <td>To verify bi-stable function of eukaryotic version of signal filter. </td>
 +
</tr>
 +
</table>
 +
              <p>The part collection well documents the prokaryote regulation circuit of our project with every detailed characterization of the transcriptional related proteins and promoter.</p>
 +
              <img src="https://static.igem.org/mediawiki/2016/8/88/T--HUST-China--Description-Fig-Eukaryote.png" alt="">
 +
              <p>The whole circuit employs bacteriaphage lambda operon and is redesigned as a positive feedback tri-stable switch. Users can define two inputs and three kinds of outputs to make a sense-response system or an analysis-report system. </p>
 +
              <img src="https://static.igem.org/mediawiki/2016/7/70/T--HUST-China--Logic-gate.jpg" alt="">
 +
              <p>To characterize the internal protein-protein and protein-promoter reactions, we build four sets of test circuits:</p>
 +
              <h2>1.Cro and pRM inhibition test:</h2>
  
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                    <tr>
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<h3>★  ALERT! </h3>
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                        <td>Part Number</td>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">Part Collection special prize</a>. </p>
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                        <td>Name</td>
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                        <td>Description</td>
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                    </tr>
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                    <tr>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2036020"> BBa_K2036020 </a></td>
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</div>
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                        <td> RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag </td>
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                        <td> To construct the verification circuit.</td>
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                    </tr>
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                    <tr>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2036027"> BBa_K2036027 </a></td>
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                        <td>pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag </td>
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                        <td> To verify the function of the whole circuit with T7 and ptrp sensing the signals.</td>
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                <img src="https://static.igem.org/mediawiki/2016/f/f0/T--HUST-China--Experiments-Fig14-1.png" alt="">
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                <img src="https://static.igem.org/mediawiki/2016/e/e7/T--HUST-China--Experiments-Fig14-2.png" alt="">
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                <img src="https://static.igem.org/mediawiki/2016/4/46/T--HUST-China--Experiments-Fig14-3.png " alt="">
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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                <p>In ideal conditions, RFP is supposed to be visualized through fluorescence microscope when induced by IPTG. And after adding IAA into the medium, our host strain will turn to the other state of GFP expression. And because of LVAssrA tag’s effect, the RFP to GFP expression transition will be relatively obvious.</p>
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<p>
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                <h2>Application cicuit</h2>
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                <p>To provide an application example, we constructed an application circuit based on this version to help relief lactose intolerance (<a href="https://2016.igem.org/Team:HUST-China/Demonstrate">more details see to application page</a>) .
 
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  <td>Part Number</td>
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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  <td>Name</td>
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  <td><a href="http://parts.igem.org/Part:BBa_K2036028"> BBa_K2036028</a></td>       
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<td>placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase</td>
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  <td>To verify bi-stable function of eukaryotic version of signal filter. </td>
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<p>In the bi-stable switch of prokaryotes, we set the gene of interest 1 as iLDH and gene of interest 2 as beta-galactosidase. For sensing the signal, we choose lactose inducible promoter plac(mutant) and base inducible promoter patp2 (works when the pH of microenvironment is about 7~9).</p>
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<img src="https://static.igem.org/mediawiki/2016/a/a3/T--HUST-China--modeling-application.png" alt="">
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<p> And we hope more team to join and add more posibilities to the circuit!</p>
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Revision as of 19:50, 15 October 2016

Parts

Part collection

Following documented composite parts are respectively from three circuits this year:

Collection list

Here is the list of our part collection:

Part Number Name Description
BBa_K2036009 pRM-GFP-LVAssrAtag A control group of Cro and pRM interaction characterization
BBa_K2036010 Cro-TT-pRM-RBS-GFP-LVAssrAtag To test Cro and pRM interaction intensity.
BBa_K2036011 pRE-GFP-LVAssrAtag A control group of CII and pRE interaction
BBa_K2036013 RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag To characterize CII’s interaction with pRE.
BBa_K2036014 RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag To characterize CIII’s interaction with Ftsh.
BBa_K2036015 RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag Tandem of CII amplifies its function.
BBa_K2036016 pR-GFP-LVAssrAtag A control group of CI and pR interaction.
BBa_K2036017 CI-TT-pR-RBS-GFP-LVAssrAtag To characterize CI and pR interaction.
BBa_K2036027 pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag To verify the function of the whole circuit with T7 and ptrp sensing the signals.
BBa_K2036028 placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase To verify bi-stable function of eukaryotic version of signal filter.

The part collection well documents the prokaryote regulation circuit of our project with every detailed characterization of the transcriptional related proteins and promoter.

The whole circuit employs bacteriaphage lambda operon and is redesigned as a positive feedback tri-stable switch. Users can define two inputs and three kinds of outputs to make a sense-response system or an analysis-report system.

To characterize the internal protein-protein and protein-promoter reactions, we build four sets of test circuits:

1.Cro and pRM inhibition test:

Part Number Name Description
BBa_K2036020 RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag To construct the verification circuit.
BBa_K2036027 pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag To verify the function of the whole circuit with T7 and ptrp sensing the signals.

In ideal conditions, RFP is supposed to be visualized through fluorescence microscope when induced by IPTG. And after adding IAA into the medium, our host strain will turn to the other state of GFP expression. And because of LVAssrA tag’s effect, the RFP to GFP expression transition will be relatively obvious.

Application cicuit

To provide an application example, we constructed an application circuit based on this version to help relief lactose intolerance (more details see to application page) .

Part Number Name Description
BBa_K2036028 placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase To verify bi-stable function of eukaryotic version of signal filter.

In the bi-stable switch of prokaryotes, we set the gene of interest 1 as iLDH and gene of interest 2 as beta-galactosidase. For sensing the signal, we choose lactose inducible promoter plac(mutant) and base inducible promoter patp2 (works when the pH of microenvironment is about 7~9).

And we hope more team to join and add more posibilities to the circuit!