Difference between revisions of "GDSYZX-United/PROJECT/enzyme cleavage"
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<p><div class="text_font"><strong>Place:</strong>Biology Experiment Teaching Center Of Sun Yat-sen University</div></p> | <p><div class="text_font"><strong>Place:</strong>Biology Experiment Teaching Center Of Sun Yat-sen University</div></p> | ||
| − | + | <p><div class="text_font"><strong>Experimental drugs: </strong>PCR products, Bsa1 restriction enzyme,Pst1restriction enzyme,Ecor1 restriction enzyme,Not1 restriction enzyme,10×buffer solution, ddH2O</div></p> | |
| − | <p><div class="text_font"><strong>Experimental drugs: </strong>PCR products, Pst1restriction enzyme,10×buffer solution, ddH2O</div></p> | + | |
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Revision as of 06:15, 16 October 2016
Enzyme cleavage
Place:Biology Experiment Teaching Center Of Sun Yat-sen University
Experimental drugs: PCR products, Bsa1 restriction enzyme,Pst1restriction enzyme,Ecor1 restriction enzyme,Not1 restriction enzyme,10×buffer solution, ddH2O
Procedure:
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enzyme cleavage:configuration
Blending, keeping in 37℃ for 1.5-3hPCR products (DNA) 5 μl 10×buffer solution 10 μl Pst1restriction enzyme 0.8μl ddH2O 84.2 μl -
electrophoresis
Take out 5μ DNA after enzyme cleavage to electrophoresis.
