Difference between revisions of "Team:Tianjin"
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Revision as of 09:57, 2 July 2016
A brief description
This March our team paid much attention to an article ‘A bacterium that degrades and assimilates poly (ethylene terephthalate)’published in Science in the same month. A new kind of bacteria that can decompose PET was found and studied in detail. We plan to express its unique genes in some commonly used mode organisms such as yeasts and E.colis to enhance its activities of decomposition significantly since they are relatively low at present.
Plastics
A brief description
Background:
This March our team paid much attention to an article ‘A bacterium that degrades and assimilates poly (ethylene terephthalate)’published in Science in the same month. A new kind of bacteria that can decompose PET was found and studied in detail. We plan to express its unique genes in some commonly used mode organisms such as yeasts and E.colis to enhance its activities of decomposition significantly since they are relatively low at present.
Current situation:
We have synthesized the gene sequences of the PETase and MHETase based on the supplementary materials of the original paper after several months’ literature reviewing. And we began several preliminary experiments to figure out if those exogenous genes could be well expressed in the host cells. We decide to enhance the activities of these two enzymes via surface display, protein scaffold and fusion expression. Another way to enhance the rate of reactions is to put the first (hydrolysis of PET) and the second step (hydrolysis of MHET) together by cascade catalysis.
Vision:
We hope to construct a system that can efficiently express and secrete (or display) these two enzymes. The system will be able to hydrolyze PET with a much higher rate than the Ideonella sakaiensis reported in the thesis.
Before you start:
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
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Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
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- You have a global audience! Consider the different backgrounds that your users come from.
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- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2016 calendar
- Have lots of fun!
Inspiration
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