Difference between revisions of "Team:BostonU/Composite Part"

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To create the Gemini parts library we made use of two “classes” of plasmid: guide RNA expressing vectors and guide RNA operator reporter vectors. When these two plasmids are co-transfected with a dCas9-VPR plasmid into a mammalian cell line, the production of a gene of interest proceeds. The genes of interest in our work were fluorescent proteins; specifically GFP was utilized in the guide RNA operator reporter vectors submitted to the registry. The guide RNA operator vector can be considered a composite part. It is constructed by combining the following parts in order: BBa_K1875009 (the target sequence), BBa_K1875000 (the miniCMV), BBa_K1875001 (the Kozak sequence), BBa_K1875003 (the GFP), BBa_K1875002 (the Rabbit Beta Globin PolyA). The sources of these parts can be found on their respective registry pages. We have submitted <a href = "http://parts.igem.org/Part:BBa_K1875018" style = "color:blue;">BBa_K1875018</a> for best composite part due to both its high expression and consistency in experimentation. Specifically, this part, when compared with a CMV, can express GFP at a high level than the strong promoter. This means that this part grants a scientist the ability to produce a protein at a higher level than a strong promoter and grant them a temporal control.
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To create the Gemini parts library we made use of two “classes” of plasmid: guide RNA expressing vectors and guide RNA operator reporter vectors. When these two plasmids are co-transfected with a dCas9-VPR plasmid into a mammalian cell line, a gene of interest is expressed. <b>Our work for iGEM dealt with fluorescent proteins; specifically, GFP for the parts submitted to the registry.<b>  </p>
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The guide RNA operator vector can be considered a composite part. It is constructed by combining the following basic parts in order: BBa_K1875009 (20 bp target sequence), BBa_K1875000 (minimal CMV promoter), BBa_K1875001 (Kozak sequence), BBa_K1875003 (GFP), BBa_K1875002 (Rabbit Beta Globin PolyA terminator). The sources of these parts can be found on their respective registry pages.  
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Revision as of 23:25, 18 October 2016

Best New Composite Part


To create the Gemini parts library we made use of two “classes” of plasmid: guide RNA expressing vectors and guide RNA operator reporter vectors. When these two plasmids are co-transfected with a dCas9-VPR plasmid into a mammalian cell line, a gene of interest is expressed. Our work for iGEM dealt with fluorescent proteins; specifically, GFP for the parts submitted to the registry.

The guide RNA operator vector can be considered a composite part. It is constructed by combining the following basic parts in order: BBa_K1875009 (20 bp target sequence), BBa_K1875000 (minimal CMV promoter), BBa_K1875001 (Kozak sequence), BBa_K1875003 (GFP), BBa_K1875002 (Rabbit Beta Globin PolyA terminator). The sources of these parts can be found on their respective registry pages.