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Revision as of 13:32, 19 October 2016
WHAT IS SIGNAL FILTER ?
This year, we, HUST-China, design a functional gene expression toolkit. The systems we design can not only be adaptable to any input and output, but also can change its threshold to meet the requirements from different project purposes.
![](https://static.igem.org/mediawiki/2016/7/7e/T--HUST-China--Home-2-Box1.png)
Normally, gene expression level depends on the input signals' intensity but it always cna't be strong enough, so it may be hard for former expression systems to deal with realistic problems.
![](https://static.igem.org/mediawiki/2016/a/a3/T--HUST-China--Home-2-modle.png)
![](https://static.igem.org/mediawiki/2016/c/cf/T--HUST-China--Home-2-Box2.png)
Our toolkit can sense even pulse signal to reach its stable expression state and with validly characterized circuits, it will definitely help new iGEMers.
WHY DO WE USE IT ?
This year, we, HUST-China, design a functional gene expression toolkit. The systems we design can not only be adaptable to any input and output, but also can change its threshold to meet the requirements from different project purposes.
![](https://static.igem.org/mediawiki/2016/7/7e/T--HUST-China--Home-2-Box1.png)
Normally, gene expression level depends on the input signals' intensity but it always cna't be strong enough, so it may be hard for former expression systems to deal with realistic problems.
![](https://static.igem.org/mediawiki/2016/a/a3/T--HUST-China--Home-2-modle.png)
![](https://static.igem.org/mediawiki/2016/c/cf/T--HUST-China--Home-2-Box2.png)
Our toolkit can sense even pulse signal to reach its stable expression state and with validly characterized circuits, it will definitely help new iGEMers.
HOW DOES IT WORK ?
This year, we, HUST-China, design a functional gene expression toolkit. The systems we design can not only be adaptable to any input and output, but also can change its threshold to meet the requirements from different project purposes.
![](https://static.igem.org/mediawiki/2016/7/7e/T--HUST-China--Home-2-Box1.png)
Normally, gene expression level depends on the input signals' intensity but it always cna't be strong enough, so it may be hard for former expression systems to deal with realistic problems.
![](https://static.igem.org/mediawiki/2016/a/a3/T--HUST-China--Home-2-modle.png)
![](https://static.igem.org/mediawiki/2016/c/cf/T--HUST-China--Home-2-Box2.png)
When signal 1 (ON) comes, promoter RD29A drives expression of SnRK2.2, which can phosphorylate a large quantity of ABF2 in a short time by enzyme catalysis , then enhance pRD29A to turn on the gene of interest's expression. When signal 2 (OFF) comes, gene PP2CA expresses, dephosphorylates ABF2 and inactivates SnRK2.2, thus turning the system into OFF state.