Difference between revisions of "Team:Harvard BioDesign/Basic Part"

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<p>The pelB tag is an N-terminus secretion tag that transports a protein from the interior of an E. coli cell to the periplasm. The tag associated with pelB secretes proteins to the periplasm of the cell, the space between the interior of the cell and the extracellular medium. Although this secretion method does not allow the protein to be excreted completely, this tag has been shown to be efficient and reliable, and still allows for secretion because tagged proteins are able to diffuse from the periplasm to the extracellular medium.</p>
 
<p>The pelB tag is an N-terminus secretion tag that transports a protein from the interior of an E. coli cell to the periplasm. The tag associated with pelB secretes proteins to the periplasm of the cell, the space between the interior of the cell and the extracellular medium. Although this secretion method does not allow the protein to be excreted completely, this tag has been shown to be efficient and reliable, and still allows for secretion because tagged proteins are able to diffuse from the periplasm to the extracellular medium.</p>
  
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Revision as of 02:34, 20 October 2016

Harvard BioDesign 2016

Basic Parts

Basic Parts

Basic Parts are parts that serve one specific function, but require other parts to produce protein or perform other functions.

T7 Promoter

T7 Express lysY/Iq Competent E. coli.

Source: BBa_I712074

RBS 34

BBa_B0034

PETase

E. coli K12 optimized with the Codon Optimization Tool from the IDT website.

Find out more about PETase on our project description under I. Sakaiensis

Uniprot Source

sfGFP

Wu, Xudong, Di Wu, Zhisheng Lu, Wentao Chen, Xiaojian Hu, and Yu Ding. "A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag." Journal of Biomedicine and Biotechnology 2009 (2009): 1-8. Web.

ompT

 Yoon, Sung, Seong Kim, and Jihyun Kim. "Secretory Production of Recombinant Proteins in Escherichia Coli." BIOT Recent Patents on Biotechnology 4.1 (2010): 23-29. Web.

YebF

Linkers

Chen, Xiaoying, Jennica L. Zaro, and Wei-Chiang Shen. "Fusion Protein Linkers: Property, Design and Functionality." Advanced Drug Delivery Reviews 65.10 (2013): 1357-369. Web.

His-Tag