Basic Parts
Basic Parts
Basic Parts are parts that serve one specific function, but require other parts to produce protein or perform other functions.
T7 Promoter
T7 Express lysY/Iq Competent E. coli.
Source: BBa_I712074
RBS 34
BBa_B0034
PETase
 degrading enzyme. It breaks down PET into ethylene glycol and terephthalic acid. Originally named ISF6_4831, PETase comes from <em>Ideonella sakaiensis,</em> identified in )
E. coli K12 optimized with the Codon Optimization Tool from the IDT website.
Find out more about PETase on our project description under I. Sakaiensis
sfGFP
 is a common method of visualizing proteins in order to detect their presence, through basic fluorescent assays. When proteins are fused to GFP, the presence of GFP most likely indicates the presence of the proteins fused to GFP. sfGFP, or super-folded GFP, is a variant of GFP designed to help fold polypeptide chains into proteins. sfGFP also increases the solubility of fused proteins, as indicated by Wu et al. in )
Wu, Xudong, Di Wu, Zhisheng Lu, Wentao Chen, Xiaojian Hu, and Yu Ding. "A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag." Journal of Biomedicine and Biotechnology 2009 (2009): 1-8. Web.
ompT
Yoon, Sung, Seong Kim, and Jihyun Kim. "Secretory Production of Recombinant Proteins in Escherichia Coli." BIOT Recent Patents on Biotechnology 4.1 (2010): 23-29. Web.
YebF
. The YebF secretion method has been shown to excrete protein completely and reliably, though it should be noted that YebF may cause steric inhibition that can disrupt the function of the fused target protein.</p>
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Zhang, Guijin, Stephen Brokx, and Joel H. Weiner. )
Linkers
Chen, Xiaoying, Jennica L. Zaro, and Wei-Chiang Shen. "Fusion Protein Linkers: Property, Design and Functionality." Advanced Drug Delivery Reviews 65.10 (2013): 1357-369. Web.
His-Tag
