Difference between revisions of "Team:Harvard BioDesign/Composite Part"

Revision as of 02:50, 20 October 2016

Harvard BioDesign 2016

Composite Parts

are parts made of basic parts that combine the functions of each basic part to provide a higher-order function, such as the production PETase.

The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon.

Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase.

Overall, there are 8 composite parts, which consist of the following:

BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon

BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon

BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon

BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon

BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon

BBa_K201005: T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon

BBa_K201007: T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon

BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon

@@ Line 138: / Line 138: @@
 <p>Overall, there are 8 composite parts, which consist of the following:</p>
-<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/d/dc/T--Harvard_BioDesign--BBa_K2010000.png/1600px-T--Harvard_BioDesign--BBa_K2010000.png”/></a>
+<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/d/dc/T--Harvard_BioDesign--BBa_K2010000.png/1600px-T--Harvard_BioDesign--BBa_K2010000.png"/></a>
 <p><a href="http://parts.igem.org/Part:BBa_K2010000"><b>BBa_K201000:</b> T7 Promoter + PETase + His-Tag + Stop Codon</a></p>
-<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/b/be/T--Harvard_BioDesign--BBa_K2010001.png/1600px-T--Harvard_BioDesign--BBa_K2010001.png”/></a>
+<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/b/be/T--Harvard_BioDesign--BBa_K2010001.png/1600px-T--Harvard_BioDesign--BBa_K2010001.png"/></a>
 <p><a href="http://parts.igem.org/Part:BBa_K2010001"><b>BBa_K201001:</b> T7 Promoter + ompT + PETase + His-Tag + Stop Codon</a></p>
-<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/9/94/T--Harvard_BioDesign--BBa_K2010002.png/1596px-T--Harvard_BioDesign--BBa_K2010002.png”/></a>
+<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/9/94/T--Harvard_BioDesign--BBa_K2010002.png/1596px-T--Harvard_BioDesign--BBa_K2010002.png"/></a>
 <p><a href="http://parts.igem.org/Part:BBa_K2010002"><b>BBa_K201002:</b> T7 Promoter + pelB + PETase + His-Tag + Stop Codon</a></p>
-<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/1/19/T--Harvard_BioDesign--BBa_K2010003.png/1600px-T--Harvard_BioDesign--BBa_K2010003.png”/></a>
+<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/1/19/T--Harvard_BioDesign--BBa_K2010003.png/1600px-T--Harvard_BioDesign--BBa_K2010003.png"/></a>
 <p><a href="http://parts.igem.org/Part:BBa_K2010003"><b>BBa_K201003:</b> T7 Promoter + YebF + PETase + His-Tag + Stop Codon</a></p>
-<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/6/6d/T--Harvard_BioDesign--BBa_K2010004.png/1600px-T--Harvard_BioDesign--BBa_K2010004.png”/></a>
+<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/6/6d/T--Harvard_BioDesign--BBa_K2010004.png/1600px-T--Harvard_BioDesign--BBa_K2010004.png"/></a>
 <p><a href="http://parts.igem.org/Part:BBa_K2010004"><b>BBa_K201004:</b> T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon</a></p>
-<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/9/90/T--Harvard_BioDesign--BBa_K2010005.png/1598px-T--Harvard_BioDesign--BBa_K2010005.png”/></a>
+<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/9/90/T--Harvard_BioDesign--BBa_K2010005.png/1598px-T--Harvard_BioDesign--BBa_K2010005.png"/></a>
 <p><a href="http://parts.igem.org/Part:BBa_K2010005"><b>BBa_K201005:</b> T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon</a></p>
-<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/d/d3/T--Harvard_BioDesign--BBa_K2010007.png/1598px-T--Harvard_BioDesign--BBa_K2010007.png”/></a>
+<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/d/d3/T--Harvard_BioDesign--BBa_K2010007.png/1598px-T--Harvard_BioDesign--BBa_K2010007.png"/></a>
 <p><a href="http://parts.igem.org/Part:BBa_K2010007"><b>BBa_K201007:</b> T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon</a></p>
-<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/c/c9/T--Harvard_BioDesign--BBa_K2010008.png/1595px-T--Harvard_BioDesign--BBa_K2010008.png”/></a>
+<a href="#" class="image featured"><img src="https://static.igem.org/mediawiki/2016/thumb/c/c9/T--Harvard_BioDesign--BBa_K2010008.png/1595px-T--Harvard_BioDesign--BBa_K2010008.png"/></a>
 <p><a href="http://parts.igem.org/Part:BBa_K2010008"><b>BBa_K201008:</b> T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon</a></p>
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