Team:Hong Kong UCCKE/Results

Result of Diacetyl Volatile Test



For the diacetyl volatile test, 6 sets of the tests were performed using different concentrations of diacetyl. Each set was repeated twice to obtain an average responsive index to maximize accuracy. For 1x concentration of diacetyl, the average index was 0.96. At 1/10x concentration, the average index dropped slightly to 0.84. From then on, it dropped dramatically to 0.27 at 1/100x concentration, then to 0.17 at 1/1000x concentration. At 1/10000x concentration, the index was at 0.05. but it rose slightly to 0.06 at 1/100000x concentration.

All the indices collected were positive. This suggests that diacetyl is a volatile attractant. The high index recorded at 1x concentration shows that diacetyl is the most potent attractant. However, as evidenced by data from 1x concentration to 1/1000x concentration, when concentration is lowered, its potency as an attractant decreases. It can be concluded, hence, that the potency of diacetyl as an attractant is directly proportionate to its concentration up till 1/1000x concentration. The slight rise in average index at 1/100000x concentration suggests that 1/100000x diacetyl is a slightly stronger attractant than 1/10000x diacetyl.

As a result, this assay proves that pure diacetyl attracts C. elegans and can be utilized in our experiments with transformed bacteria.

Chemical Type Concentration # of worms in T # of worms in C Index
Diacetyl Volatile Test 1 10 0 1
Diacetyl Volatile Test 1 80 3 0.927710843
Diacetyl Volatile Test 1 96 2 0.959183673
Diacetyl Volatile Test 1/10 97 8 0.847619048
Diacetyl Volatile Test 1/10 78 9 0.793103448
Diacetyl Volatile Test 1/10 91 6 0.87628866
Diacetyl Volatile Test 1/100 7 4 0.272727273
Diacetyl Volatile Test 1/100 85 54 0.223021583
Diacetyl Volatile Test 1/100 60 32 0.304347826
Diacetyl Volatile Test 1/1000 18 14 0.125
Diacetyl Volatile Test 1/1000 67 45 0.196428571
Diacetyl Volatile Test 1/1000 51 35 0.186046512
Diacetyl Volatile Test 1/10000 13 13 0
Diacetyl Volatile Test 1/10000 27 22 0.102040816
Diacetyl Volatile Test 1/10000 27 24 0.058823529
Diacetyl Volatile Test 1/100000 18 16 0.058823529
Diacetyl Volatile Test 1/100000 32 29 0.049180328
Diacetyl Volatile Test 1/100000 35 31 0.060606061

Result of Cinnemaldehyde Volatile Test

Chemical Type Concentration # of worms in T # of worms in C Index
Cinnemoaldehyde Volatile Test 1 18 75 -0.612903226
Cinnemoaldehyde Volatile Test 1 0 24 -1
Cinnemoaldehyde Volatile Test 1 11 92 -0.786407767
Cinnemoaldehyde Volatile Test 1 9 95 -0.826923077
Cinnemoaldehyde Volatile Test 1/10 10 97 -0.813084112
Cinnemoaldehyde Volatile Test 1/10 3 32 -0.828571429
Cinnemoaldehyde Volatile Test 1/10 13 93 -0.754716981
Cinnemoaldehyde Volatile Test 1/10 11 98 -0.798165138
Cinnemoaldehyde Volatile Test 1/100 6 90 -0.875
Cinnemoaldehyde Volatile Test 1/100 2 38 -0.9
Cinnemoaldehyde Volatile Test 1/100 9 68 -0.766233766
Cinnemoaldehyde Volatile Test 1/100 14 96 -0.745454545
Cinnemoaldehyde Volatile Test 1/1000 27 82 -0.504587156
Cinnemoaldehyde Volatile Test 1/1000 9 28 -0.513513514
Cinnemoaldehyde Volatile Test 1/1000 14 40 -0.481481481
Cinnemoaldehyde Volatile Test 1/1000 17 50 -0.492537313
Cinnemoaldehyde Volatile Test 1/10000 40 35 0.066666667
Cinnemoaldehyde Volatile Test 1/10000 16 28 -0.272727273
Cinnemoaldehyde Volatile Test 1/10000 27 37 -0.15625
Cinnemoaldehyde Volatile Test 1/10000 29 39 -0.147058824
Cinnemoaldehyde Volatile Test 1/100000 34 44 -0.128205128
Cinnemoaldehyde Volatile Test 1/100000 25 37 -0.193548387
Cinnemoaldehyde Volatile Test 1/100000 28 33 -0.081967213
Cinnemoaldehyde Volatile Test 1/100000 32 33 -0.015384615

Overall Comparison