Team:Hong Kong UCCKE/Results
Result of Diacetyl Volatile Test
For the diacetyl volatile test, 6 sets of the tests were performed using different concentrations of diacetyl. Each set was repeated twice to obtain an average responsive index to maximize accuracy. For 1x concentration of diacetyl, the average index was 0.96. At 1/10x concentration, the average index dropped slightly to 0.84. From then on, it dropped dramatically to 0.27 at 1/100x concentration, then to 0.17 at 1/1000x concentration. At 1/10000x concentration, the index was at 0.05. but it rose slightly to 0.06 at 1/100000x concentration.
All the indices collected were positive. This suggests that diacetyl is a volatile attractant. The high index recorded at 1x concentration shows that diacetyl is the most potent attractant. However, as evidenced by data from 1x concentration to 1/1000x concentration, when concentration is lowered, its potency as an attractant decreases. It can be concluded, hence, that the potency of diacetyl as an attractant is directly proportionate to its concentration up till 1/1000x concentration. The slight rise in average index at 1/100000x concentration suggests that 1/100000x diacetyl is a slightly stronger attractant than 1/10000x diacetyl.
As a result, this assay proves that pure diacetyl attracts C. elegans and can be utilized in our experiments with transformed bacteria.
| Chemical | Type | Concentration | # of worms in T | # of worms in C | Index |
|---|---|---|---|---|---|
| Diacetyl | Volatile Test | 1 | 10 | 0 | 1 |
| Diacetyl | Volatile Test | 1 | 80 | 3 | 0.927710843 |
| Diacetyl | Volatile Test | 1 | 96 | 2 | 0.959183673 |
| Diacetyl | Volatile Test | 1/10 | 97 | 8 | 0.847619048 |
| Diacetyl | Volatile Test | 1/10 | 78 | 9 | 0.793103448 |
| Diacetyl | Volatile Test | 1/10 | 91 | 6 | 0.87628866 |
| Diacetyl | Volatile Test | 1/100 | 7 | 4 | 0.272727273 |
| Diacetyl | Volatile Test | 1/100 | 85 | 54 | 0.223021583 |
| Diacetyl | Volatile Test | 1/100 | 60 | 32 | 0.304347826 |
| Diacetyl | Volatile Test | 1/1000 | 18 | 14 | 0.125 |
| Diacetyl | Volatile Test | 1/1000 | 67 | 45 | 0.196428571 |
| Diacetyl | Volatile Test | 1/1000 | 51 | 35 | 0.186046512 |
| Diacetyl | Volatile Test | 1/10000 | 13 | 13 | 0 |
| Diacetyl | Volatile Test | 1/10000 | 27 | 22 | 0.102040816 |
| Diacetyl | Volatile Test | 1/10000 | 27 | 24 | 0.058823529 |
| Diacetyl | Volatile Test | 1/100000 | 18 | 16 | 0.058823529 |
| Diacetyl | Volatile Test | 1/100000 | 32 | 29 | 0.049180328 |
| Diacetyl | Volatile Test | 1/100000 | 35 | 31 | 0.060606061 |
Result of Cinnemaldehyde Volatile Test
| Chemical | Type | Concentration | # of worms in T | # of worms in C | Index |
|---|---|---|---|---|---|
| Cinnemoaldehyde | Volatile Test | 1 | 18 | 75 | -0.612903226 |
| Cinnemoaldehyde | Volatile Test | 1 | 0 | 24 | -1 |
| Cinnemoaldehyde | Volatile Test | 1 | 11 | 92 | -0.786407767 |
| Cinnemoaldehyde | Volatile Test | 1 | 9 | 95 | -0.826923077 |
| Cinnemoaldehyde | Volatile Test | 1/10 | 10 | 97 | -0.813084112 |
| Cinnemoaldehyde | Volatile Test | 1/10 | 3 | 32 | -0.828571429 |
| Cinnemoaldehyde | Volatile Test | 1/10 | 13 | 93 | -0.754716981 |
| Cinnemoaldehyde | Volatile Test | 1/10 | 11 | 98 | -0.798165138 |
| Cinnemoaldehyde | Volatile Test | 1/100 | 6 | 90 | -0.875 |
| Cinnemoaldehyde | Volatile Test | 1/100 | 2 | 38 | -0.9 |
| Cinnemoaldehyde | Volatile Test | 1/100 | 9 | 68 | -0.766233766 |
| Cinnemoaldehyde | Volatile Test | 1/100 | 14 | 96 | -0.745454545 |
| Cinnemoaldehyde | Volatile Test | 1/1000 | 27 | 82 | -0.504587156 |
| Cinnemoaldehyde | Volatile Test | 1/1000 | 9 | 28 | -0.513513514 |
| Cinnemoaldehyde | Volatile Test | 1/1000 | 14 | 40 | -0.481481481 |
| Cinnemoaldehyde | Volatile Test | 1/1000 | 17 | 50 | -0.492537313 |
| Cinnemoaldehyde | Volatile Test | 1/10000 | 40 | 35 | 0.066666667 |
| Cinnemoaldehyde | Volatile Test | 1/10000 | 16 | 28 | -0.272727273 |
| Cinnemoaldehyde | Volatile Test | 1/10000 | 27 | 37 | -0.15625 |
| Cinnemoaldehyde | Volatile Test | 1/10000 | 29 | 39 | -0.147058824 |
| Cinnemoaldehyde | Volatile Test | 1/100000 | 34 | 44 | -0.128205128 |
| Cinnemoaldehyde | Volatile Test | 1/100000 | 25 | 37 | -0.193548387 |
| Cinnemoaldehyde | Volatile Test | 1/100000 | 28 | 33 | -0.081967213 |
| Cinnemoaldehyde | Volatile Test | 1/100000 | 32 | 33 | -0.015384615 |
