Overview About Notes

Our experiment started from July after designing and discussing for several months. Every day after finishing the experiment, we wrote down what we did this day briefly but clearly. After all the experiments came to a end, we summaried all of our experiment notes and divided them into five groups. The detailed notes can be accessed by clicking the link below.

R-R system

R-R (Reporting and regulation) system consists of the inclusion body reporting system, the inclusion body induced cell lysis system and the TPA positive feedback regulation system. The experiment of this part started at end of August and lasted for 6 weeks. Clicking this link to see details.

Consortium

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CFPS

We utilized the cell-free system to express the enzymes which had been modified in 22 different sites. Then we used the proteins successfully expressed to degrade PET. Our expected goal was to screen mutations with higher enzyme activities than the wild type PETase.

Cyanobacteria