Team:Tianjin/Achievements

Notes for Bacterial Consortium

Optimization of Culture Conditions

  Jul.26th

Prepare M9 medium with TPA and culture Pseudomonas putida KT2440 at 30℃ & 200 rpm, checked the bacterial concentration at OD₆₀₀.

  Jul.27th

1.Extract 10 ml LB medium to 5 test tubes and add 0μl, 50μl,, 100μl,, 250μl,, 500μl EG to them, respectively.
2.Add 5μl bacteria solution of Pseudomonas putida KT2440 to five test tubes above.
3.Culture them at 30℃ & 200 rpm, checked the bacterial concentration at OD₆₀₀.

  Jul.28th

Add 6g TPA, 2.9gNaOH and 1.2g glucose to M9 medium, and culture them in the improved M9 medium and M9 medium of Jul.26 at 30℃ & 200 rpm, checked the bacterial concentration at OD₆₀₀ and detected the concentration of TPA by UV at OD₂₄₂.

  Jul.30th

Cultured different bacteria in M9 medium with sodium terephthalate and different concentration of glucose at 30℃ & 200 rpm, checked the bacterial concentration at OD₆₀₀ and detected the concentration of TPA by UV at OD₂₄₂.

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