Composite Parts
The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon. Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase. Overall, there are 8 composite parts involved in Plastiback.
List of Composite Parts/h3>
BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon BBa_K201005: T7 Promoter + ompT + sfGFP + PETase + His-Tag + Stop Codon BBa_K201007: T7 Promoter + pelB + sfGFP + PETase + His-Tag + Stop Codon BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon
