Welcome to the BostonU Parts Pages. We will keep it brief here, but if you want further information we recommend you visit the various pages associated with our work in the registry.
We at BostonU decided to submit several parts from the Gemini Library. First we submitted two guide RNA expressing vectors. Designated as BBa_K1875011 and BBa_K1875012, containing the target sequence for guide RNAs 3 and 8 respectively. These parts represent our Bronze Medal part submission.
In addition to the Bronze Medal parts, we submitted a selection of guide RNA operator reporters for Silver Medal consideration. Those parts include BBa_K1875013 (single site with guide 1), BBa_K1875014 (single site with guide 3), BBa_K1875015 (single site with guide 8), BBa_K1875016(single site with guide 13), BBa_K1875017 (double site with guide 13), BBa_K1875018 (triple site with guide 13), BBa_K1875019 (mutated guide 13).
The above parts are all considered composite parts, and thus a collection of basic parts was pages was created on the Registry. These include the various target sequences, the minimal CMV promoter, a Kozak sequence, a GFP protein, and a Rabbit Beta Globin Poly A terminator. These parts have the ID numbers BBa_K1875000 - BBa_K1875010. These parts do not have samples in the registry, but their sequences are all available on their respective Registry pages.