Parts For R-R System

Biobrick	Type	Description	Length
BBa_K2110000	Basic	CYC1 Terminator	248
BBa_K2110012	Basic	PGK1 promoter	781
BBa_K2110017	Basic	α-factor signal peptide	267
BBa_K2110001	Basic	TPA inducible Leader sequence	96
BBa_K2110002	Basic	TPA positive feedback regulatory gene	807
BBa_K2110003	Basic	TPA transporting protein gene	1380
BBa_K2110004	Basic	D-alanyl-D-alanine dipeptidase gene	582
BBa_K2110005	Composite	TPA inducible PETase expression system	2111
BBa_K2110006	Composite	TPA regulatory gene expression system	1850
BBa_K2110007	Composite	TPA transporting protein gene expression system	2425
BBa_K2110008	Composite	inclusion body induced cell lysis system	665

Parts For Microbial Consortium

Biobrick	Type	Description	Length
BBa_K2110009	Basic	phage P22 holins	327
BBa_K2110010	Basic	phage P22 auxiliary lysis factors	438
BBa_K2110011	Basic	phage P22 endolysins	441
BBa_K2110013	Basic	PETase enzyme after codon optimization for bacillus subtilis	885
BBa_K2110014	Basic	MHETase enzyme after codon optimization for bacillus subtilis	1848
BBa_K2110015	Basic	The key gene for accumulation of PHA in P.putida KT2440	978
BBa_K2110016	Basic	The key gene for utilization of ethylene glycolin P.putida KT2440	1326

Biobrick	Type	Description	Length
BBa_K2110800	Basic	PETase	795
BBa_K2110100	Basic	PETase site-directed mutant A240P	795
BBa_K2110101	Basic	PETase site-directed mutant S207T	795
BBa_K2110102	Basic	PETase site-directed mutant M154L	795
BBa_K2110103	Basic	PETase site-directed mutant N205V	795
BBa_K2110104	Basic	PETase site-directed mutant R90A	795
BBa_K2110105	Basic	PETase site-directed mutant I208V	795
BBa_K2110106	Basic	PETase site-directed mutant S238V	795
BBa_K2110107	Basic	PETase site-directed mutant L117F	795
BBa_K2110108	Basic	PETase site-directed mutant I208F	795
BBa_K2110109	Basic	PETase site-directed mutant S238F	795
BBa_K2110200	Composite	PETase expression system in Saccharomyces cerevisiae	1961