Team:Uppsala/HP/Gold

Human Practices


As a part of Human Practices we decided to develop a manual for manufacturing a microfluidic chip for heat shock transformation. Hopefully this manual can make microfluidic chips easier and more available for other iGEM teams and other small labs. The manual covers the full manufacturing process for a microfluidic chip based on a 3D- printed mold. It includes all required materials, step-by-step protocols for the making of the chip and also a protocol for cell transformation on the chip. To make an example this manual is made specifically for heat shock transformation, but the design of the chip can be altered to fit any other purpose.