Team:BostonU/Composite Part
To create the Gemini parts library we made use of two “classes” of plasmid: guide RNA expressing vectors and guide RNA operator reporter vectors. When these two plasmids are co-transfected with a dCas9-VPR plasmid into a mammalian cell line, a gene of interest is expressed. Our work for iGEM dealt with fluorescent proteins; specifically, GFP for the parts submitted to the registry.
The guide RNA operator vector can be considered a composite part. It is constructed by combining the following basic parts in order: BBa_K1875009 (20 bp target sequence), BBa_K1875000 (minimal CMV promoter), BBa_K1875001 (Kozak sequence), BBa_K1875003 (GFP), BBa_K1875002 (Rabbit Beta Globin PolyA terminator). The sources of these parts can be found on their respective registry pages.
