Team:XMU-China/Results

Results

Appropriate attribution is half of success.

Entire circuits test

In our experiment, for testing our circuit more conveniently, we use the cfp to replace the responder and use gfp to replace lysis. Because the fluorescence intensity is easier to be measured. Also, the intensity of the fluorescence can stand for the intensity of the corresponding genes' expression.

When AHL around the engineered bacteria is at low concentration, AHL can't be sensed by the Lux sensor part, and the cI gene is expressed at the wild-type levels. While lacI-2 and GFP are repressed since the CI protein is at high concentration. When AHL around the engineered bacteria reach a certain concentration, AHL can be detected by the sensor part. So the promoter LuxpR is activ