Constructed BioBricks for PETase and confirmed presence of protein using sfGFP
Constructed BioBricks with secretion systems for PETase
Validated PETase expression through SDS Page and Western blot
Validated PETase enzymatic activity through pNPB substrate assay
Characterized growth of three bacteria species in TPA minimal media
Optimized PH for growth and PETase activity
Demonstrated TPA signal can be detected by voltage increase in microbial fuel cell
MEDAL REQUIREMENTS
BRONZE
1. REGISTER AND ATTEND ✓
2. DELIVERABLES ✓
We met all of the deliverable requirements: team wiki, poster, presentation, project attribution, registry parts page, sample submission, safety forms, and judging forms.
Our team has identified, investigated, and addressed issues relating to safety, sustainability, and education. Read about it here!
GOLD
1. INTEGRATED HUMAN PRACTICES ✓
The design and direction of our project were directly influenced by the feedback we received from both The Seabin Project and microbial fuel cell experts we interviewed throughout the duration of our project. (For example, The Seabin Project brought to our attention real-life factors typically unaccounted for in a lab setting, such as the variance in ocean plastic age; from there, we obtained plastic collected from a beach cleanup for experimental use. Moreover, after interviewing Professor Peter Girguis, Ph.D. we modified the design of our project, particularly the usage of the electrical output of our microbial fuel cell.) Read more about it here!
2. IMPROVE A PREVIOUS PART OR PROJECT ✓
We further characterized Team UC Davis’ (2012) part
BBa_K936000
