Team:BostonU/Proof
Phase 1
The goal of phase 1 to establish a consistent method of gene activation through dCAS9-VPR. Using MIT's CRISPR optimization tool, we generated 20 sequences to be used as our gRNA and target site. The dCAS9-VPR complex, the target sequence and reporter gene, and the gRNA expression vectors were constructed and transfected into HEK293 cells. Another set of transfections took place simultaneously with the same materials minus the gRNA expression vector as a negative control. The fold increase between the basal level of expression from the control and the activated level of expression was then recorded. The results can be found below.
The goal of phase 1 to establish a consistent method of gene activation through dCAS9-VPR. Using MIT's CRISPR optimization tool, we generated 20 sequences to be used as our gRNA and target site. The dCAS9-VPR complex, the target sequence and reporter gene, and the gRNA expression vectors were constructed and transfected into HEK293 cells. Another set of transfections took place simultaneously with the same materials minus the gRNA expression vector as a negative control. The fold increase between the basal level of expression from the control and the activated level of expression was then recorded. The results can be found below. Here are some extra words to make it seem like this is a different paragraph than the rest when really, it's not. It's just the same thing. But I want to make it look different, so there you go. Let's just add a few more words, and there we go. That should do it.
The goal of phase 1 to establish a consistent method of gene activation through dCAS9-VPR. Using MIT's CRISPR optimization tool, we generated 20 sequences to be used as our gRNA and target site. The dCAS9-VPR complex, the target sequence and reporter gene, and the gRNA expression vectors were constructed and transfected into HEK293 cells. Another set of transfections took place simultaneously with the same materials minus the gRNA expression vector as a negative control. The fold increase between the basal level of expression from the control and the activated level of expression was then recorded. The results can be found below.
Phase 2
Words will go here that explain all the experiments we conducted to achieve aim one. I don't know what those words will be yet, but hey, I'm just the html guy not the writer guy. I mean, I do enjoy writing, but right now I have other things to work on. I just have to remember to come back and change this part. If I forget, that would bad, but I don't think I will. Here are some extra words to make it seem like this is a different pargraph than the rest when really, it's not. It's just the same thing. But I want to make it look different, so there you go. Let's just add a few more words, and there we go. That should do it. Shoot, this still isn't enough words. Hold on, here a few more, and a few more. Okay, now I'm out of ideas.
Phase 3
These words have to be a little different, just to show that the words are changing. Everything else from here on in will be identical to phase though. So without further ado, here's phase 2 again. Words will go here that explain all the experiments we conducted to achieve aim one. I don't know what those words will be yet, but hey, I'm just the html guy not the writer guy. I mean, I do enjoy writing, but right now I have other things to work on. I just have to remember to come back and change this part. If I forget, that would bad, but I don't think I will. Here are some extra words to make it seem like this is a different pargraph than the rest when really, it's not. It's just the same thing. But I want to make it look different, so there you go.
