Our bacteria express two fusion proteins: antimicrobial peptide LL-37 fused to motor protein yebf through a linker, and stromal-derived factor SDF fused with elastin-like-protein composed of pentapeptides repeated for 20 times ELP(20). We first designed the sequence of each using Snapgene. Then to ensure the function of each protein, we used Phyre 2 to predict the proteins’ three-dimensional structure.
Figure 1: Diagram for DNA sequence design of SDF-ELP(20) fusion protein
Figure 2: Predicted protein structure of SDF-ELP(20)
Figure 3: Predicted protein structure of yebf-ll37
We construct the plasmids: J04500 + yebf-LL-37 + B0015 and J04500 + SDF-ELP(20) + B0015. Then we synthesize them from GenScript. The electrophoresis results of these plasmids are as followings:
Figure 4: DNA electrophoresis result of J04500 + SDF-ELP(20) + B0015.
Back to top
Figure 5: DNA electrophoresis result of J04500 + yebf-LL-37 + B0015
©2016 Shenzhen Middle School iGEM. All rights reserved.