Team:SVCE CHENNAI/Ligation

Lactoshield - SVCE_CHENNAI Lactoshield - SVCE_CHENNAI

Lactoshield - SVCE_CHENNAI Lactoshield - SVCE_CHENNAI

Ligation

Reaction mix:

  •   250-500 ng vector
  •   5-20x (in molar ratios) of insert--higher insert:vector ratio for shorter inserts
  •   2 ul T4 DNA ligase buffer
  •  1 ul DNA ligase
  •   Water to 20 ul total volume
Lactoshield - SVCE_CHENNAI Lactoshield - SVCE_CHENNAI
Lactoshield - SVCE_CHENNAI

Sponsors

igemsvce@gmail.com

Sri Venkateswara College of Engineering
Tamil Nadu, India

    

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