Team:SVCE CHENNAI/Transformation
Transformation
Requirements for each sample:
- 1 tube of thawed chemically competent cells (keep covered in ice at all times).
- 1-10 uL ligation product cloned in plasmid.
- 250 uL SOC
- 1 LB plate with appropriate antibiotics
Procedure:
- Add DNA to cells
- Incubate on ice for 0-20 minutes
- Heat shock at 42 °C for 30 seconds
- Incubate on ice for 0-2 minutes
- Add SOC
- Incubate on rotator in 37 °C incubator for 0-60 minutes
- Plate 50-250 uL
Sponsors
