Difference between revisions of "Team:NUDT CHINA/Part Collection"

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<p>
 
<p>
 
<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>Parts included in our collection</strong></span>
 
<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>Parts included in our collection</strong></span>
 +
</p>
 +
<p>
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>N-sGFP/ C-sGFP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>fluorescent </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>PPI Detector</strong></span>
 
</p>
 
</p>
 +
 +
 +
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
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<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997004" style = "color:#d82545;">BBa_K1997004</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;sGFP-N->Zif268->sGFP-C</td>
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</tr>
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<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997015" style = "color:#d82545;">BBa_K1997015</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sGFP-N->Zif268->sGFP-C->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997016" style = "color:#d82545;">BBa_K1997016</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997017" style = "color:#d82545;">BBa_K1997017</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER</td>
 +
</table>
 +
</br></br>
 +
  
 
<p>
 
<p>
−
<span style="line-height:2;font-family:Perpetua;font-size:18px;">BBa_K1997000, BBa_K1997001, BBa_K1997002, BBa_K1997003,
+
<span style="line-height:2;font-family:Perpetua;font-size:18px;"></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>GFP10/ GFP11+GFP1-9 based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>fluorescent</strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong> PPI Detector</strong></span>  
−
BBa_K1997004, BBa_K1997005, BBa_K1997012, BBa_K1997013, BBa_K1997014, BBa_K1997015,
+
−
BBa_K1997016, BBa_K1997017, BBa_K1997018, BBa_K1997019, BBa_K1997020, BBa_K1997021,
+
−
BBa_K1997022, BBa_K1997023</span>
+
 
</p>
 
</p>
  
 +
 +
 +
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997014" style = "color:#d82545;">BBa_K1997014</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;GFP10->Zif268->GFP11->RBS->GFP1-9</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997018" style = "color:#d82545;">BBa_K1997018</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->GFP10->Zif268->GFP11->RBS->GFP1-9->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997019" style = "color:#d82545;">BBa_K1997019</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->GFP10->Zif-N->RBS->Zif-C->GFP11->RBS->GFP1-9->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997020" style = "color:#d82545;">BBa_K1997020</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER</td>
 +
</table>
 +
</br></br>
 +
 +
 +
<p>
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong></strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>Split-HRP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>enzymatic </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>PPI Detector</strong></span>
 +
</p>
 +
 +
 +
 +
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997000" style = "color:#d82545;">BBa_K1997000</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;sHRP-N</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997001" style = "color:#d82545;">BBa_K1997001</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;sHRP-C</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997005" style = "color:#d82545;">BBa_K1997005</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;sHRP-N->FRB->RBS->FKBP-> sHRP-C</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997012" style = "color:#d82545;">BBa_K1997012</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sHRP-N->Zif268-> sHRP-C->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997013" style = "color:#d82545;">BBa_K1997013</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER</td>
 +
</tr>
 +
</table>
 +
</br></br>
 +
 +
 +
<p>
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong></strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>Split-Luciferase based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>enzymatic </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>PPI Detector</strong></span>
 +
</p>
 +
 +
 +
 +
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997002" style = "color:#d82545;">BBa_K1997002</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;sLuc-N</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997003" style = "color:#d82545;">BBa_K1997003</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;sLuc-C</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997021" style = "color:#d82545;">BBa_K1997021</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sLuc-N->Zif268->sLuc-C->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997022" style = "color:#d82545;">BBa_K1997022</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997023" style = "color:#d82545;">BBa_K1997023</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sLuc-N->FRB->RBS->FKBP->sLuc-C->TER</td>
 +
</tr>
 +
</table>
 +
</br></br>
  
  

Revision as of 10:32, 19 October 2016

NUDT_CHINA 2016

PAGE STILL UNDER CONSTRUCTION

PPI Toolkit

A Novel Toolkit for protein-protein interaction (PPI) study

Parts included in our collection

N-sGFP/ C-sGFP based fluorescent PPI Detector

BBa_K1997004    sGFP-N->Zif268->sGFP-C
BBa_K1997015    P+R->sGFP-N->Zif268->sGFP-C->TER
BBa_K1997016    P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER
BBa_K1997017    P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER


GFP10/ GFP11+GFP1-9 based fluorescent PPI Detector

BBa_K1997014    GFP10->Zif268->GFP11->RBS->GFP1-9
BBa_K1997018    P+R->GFP10->Zif268->GFP11->RBS->GFP1-9->TER
BBa_K1997019    P+R->GFP10->Zif-N->RBS->Zif-C->GFP11->RBS->GFP1-9->TER
BBa_K1997020    P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER


Split-HRP based enzymatic PPI Detector

BBa_K1997000    sHRP-N
BBa_K1997001    sHRP-C
BBa_K1997005    sHRP-N->FRB->RBS->FKBP-> sHRP-C
BBa_K1997012    P+R->sHRP-N->Zif268-> sHRP-C->TER
BBa_K1997013    P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER


Split-Luciferase based enzymatic PPI Detector

BBa_K1997002    sLuc-N
BBa_K1997003       sLuc-C
BBa_K1997021    P+R->sLuc-N->Zif268->sLuc-C->TER
BBa_K1997022    P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER
BBa_K1997023    P+R->sLuc-N->FRB->RBS->FKBP->sLuc-C->TER


 

Introduction

This part collection, namely PPI Tool-kit, contained 18 new parts design for protein-protein interaction (PPI) researches.

In PPI Toolkit, we collected basic parts containing the coding sequence of three split-reporting systems: split-GFP, split-Luciferase, and split-HRP. All those basic parts were codon-optimized to be expressed in E.coli, and sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining split-reporting system with a novel substitution system were designed, characterized and then added into the collection. Through which two proteins could be fused with their corresponding split-reporter fragment at the same time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning process.

All parts in this kit were functional validated, well characterized and submitted to the registry.






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