Difference between revisions of "Team:NUDT CHINA/Part Collection"

Line 407: Line 407:
 
<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>Parts included in our collection</strong></span>
 
<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>Parts included in our collection</strong></span>
 
</p>
 
</p>
 +
 +
<h2>
 +
<span><span style="color:#7f1015">Parts in our Collection</span></span><hr />
 +
</h2>
 +
 
<p>
 
<p>
 
<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>N-sGFP/ C-sGFP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>fluorescent </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>PPI Detector</strong></span>  
 
<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>N-sGFP/ C-sGFP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>fluorescent </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>PPI Detector</strong></span>  
 
</p>
 
</p>
−
 
 
 
 
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
 
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
Line 425: Line 429:
 
<td>&nbsp;&nbsp;&nbsp;P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER</td>
 
<td>&nbsp;&nbsp;&nbsp;P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER</td>
 
</table>
 
</table>
−
</br></br>
 
−
 
  
 
<p>
 
<p>
 
<span style="line-height:2;font-family:Perpetua;font-size:18px;"></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>GFP10/ GFP11+GFP1-9 based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>fluorescent</strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong> PPI Detector</strong></span>  
 
<span style="line-height:2;font-family:Perpetua;font-size:18px;"></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>GFP10/ GFP11+GFP1-9 based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>fluorescent</strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong> PPI Detector</strong></span>  
 
</p>
 
</p>
−
 
−
 
  
 
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
 
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
Line 447: Line 447:
 
<td>&nbsp;&nbsp;&nbsp;P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER</td>
 
<td>&nbsp;&nbsp;&nbsp;P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER</td>
 
</table>
 
</table>
−
</br></br>
 
−
 
  
 
<p>
 
<p>
 
<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong></strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>Split-HRP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>enzymatic </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>PPI Detector</strong></span>  
 
<span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong></strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>Split-HRP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>enzymatic </strong></span><span style="line-height:2;font-family:Perpetua;font-size:18px;"><strong>PPI Detector</strong></span>  
 
</p>
 
</p>
−
 
−
 
  
 
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
 
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
Line 473: Line 469:
 
</tr>
 
</tr>
 
</table>
 
</table>
−
</br></br>
 
−
 
  
 
<p>
 
<p>
Line 487: Line 481:
 
</tr>
 
</tr>
 
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997003" style = "color:#d82545;">BBa_K1997003</a></td>
 
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997003" style = "color:#d82545;">BBa_K1997003</a></td>
−
<td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;sLuc-C</td>
+
<td>&nbsp;&nbsp;&nbsp;sLuc-C</td>
 
</tr>
 
</tr>
 
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997021" style = "color:#d82545;">BBa_K1997021</a></td>
 
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997021" style = "color:#d82545;">BBa_K1997021</a></td>
Line 499: Line 493:
 
</tr>
 
</tr>
 
</table>
 
</table>
−
</br></br>
+
 
 +
 
 +
 
 +
 
 +
 
 +
 
  
  

Revision as of 10:35, 19 October 2016

NUDT_CHINA 2016

PAGE STILL UNDER CONSTRUCTION

PPI Toolkit

A Novel Toolkit for protein-protein interaction (PPI) study

Parts included in our collection

Parts in our Collection

N-sGFP/ C-sGFP based fluorescent PPI Detector

BBa_K1997004    sGFP-N->Zif268->sGFP-C
BBa_K1997015    P+R->sGFP-N->Zif268->sGFP-C->TER
BBa_K1997016    P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER
BBa_K1997017    P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER

GFP10/ GFP11+GFP1-9 based fluorescent PPI Detector

BBa_K1997014    GFP10->Zif268->GFP11->RBS->GFP1-9
BBa_K1997018    P+R->GFP10->Zif268->GFP11->RBS->GFP1-9->TER
BBa_K1997019    P+R->GFP10->Zif-N->RBS->Zif-C->GFP11->RBS->GFP1-9->TER
BBa_K1997020    P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER

Split-HRP based enzymatic PPI Detector

BBa_K1997000    sHRP-N
BBa_K1997001    sHRP-C
BBa_K1997005    sHRP-N->FRB->RBS->FKBP-> sHRP-C
BBa_K1997012    P+R->sHRP-N->Zif268-> sHRP-C->TER
BBa_K1997013    P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER

Split-Luciferase based enzymatic PPI Detector

BBa_K1997002    sLuc-N
BBa_K1997003    sLuc-C
BBa_K1997021    P+R->sLuc-N->Zif268->sLuc-C->TER
BBa_K1997022    P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER
BBa_K1997023    P+R->sLuc-N->FRB->RBS->FKBP->sLuc-C->TER

 

Introduction

This part collection, namely PPI Tool-kit, contained 18 new parts design for protein-protein interaction (PPI) researches.

In PPI Toolkit, we collected basic parts containing the coding sequence of three split-reporting systems: split-GFP, split-Luciferase, and split-HRP. All those basic parts were codon-optimized to be expressed in E.coli, and sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining split-reporting system with a novel substitution system were designed, characterized and then added into the collection. Through which two proteins could be fused with their corresponding split-reporter fragment at the same time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning process.

All parts in this kit were functional validated, well characterized and submitted to the registry.






No. 109 Deya Road, Kaifu District,
Changsha, Hunan Province 410073
P.R.China

SPONSORS




COPYRIGHT ©  2016 NUDT_CHINA