Difference between revisions of "Team:NUDT CHINA/Model"
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<meta name="HandheldFriendly" content="True" /> | <meta name="HandheldFriendly" content="True" /> | ||
<meta name="MobileOptimized" content="320" /> | <meta name="MobileOptimized" content="320" /> | ||
| − | <meta name="viewport" content="width=device-width, initial- | + | <meta name="viewport" content="width=device-width, initial-scale=1.0",minimum-scale=0.2, maximum-scale=2.0, user-scalable=yes" /> |
| − | + | ||
| − | scale=1.0",minimum-scale=0.2, maximum-scale=2.0, user-scalable=yes" /> | + | |
<meta content="on" http-equiv="cleartype" /> | <meta content="on" http-equiv="cleartype" /> | ||
<meta property="og:title" content="Home" /> | <meta property="og:title" content="Home" /> | ||
<meta name="generator" content="TYPO3 CMS"> | <meta name="generator" content="TYPO3 CMS"> | ||
| − | <link rel="stylesheet" type="text/css" href="/Team:NUDT_CHINA/CSS1? | + | <link rel="stylesheet" type="text/css" href="/Team:NUDT_CHINA/CSS1?action=raw&ctype=text/css" media="all"> |
| − | + | ||
| − | action=raw&ctype=text/css" media="all"> | + | |
<title>NUDT_CHINA 2016</title> | <title>NUDT_CHINA 2016</title> | ||
<meta name="title" content="iGem - NUDT_CHINA 2016" /> | <meta name="title" content="iGem - NUDT_CHINA 2016" /> | ||
<meta name="date" content="2016-10-04" /> | <meta name="date" content="2016-10-04" /> | ||
<link rel="canonical" href="http#/" /> | <link rel="canonical" href="http#/" /> | ||
| − | <!-- VhsAssetsDependenciesLoaded fontCss,mainCss,inlinejs- | + | <!-- VhsAssetsDependenciesLoaded fontCss,mainCss,inlinejs-head,jquery,mainJs,cycle4811,cycle5332,cycle4823 --> |
| − | + | <link rel="stylesheet" href="/Team:NUDT_CHINA/CSS2?action=raw&ctype=text/css" /> | |
| − | head,jquery,mainJs,cycle4811,cycle5332,cycle4823 --> | + | <link rel="stylesheet" href="/Team:NUDT_CHINA/CSS?action=raw&ctype=text/css" /></head> |
| − | <link rel="stylesheet" href="/Team:NUDT_CHINA/CSS2? | + | |
| − | + | ||
| − | action=raw&ctype=text/css" /> | + | |
| − | <link rel="stylesheet" href="/Team:NUDT_CHINA/CSS? | + | |
| − | + | ||
| − | action=raw&ctype=text/css" /></head> | + | |
<!-- body --> | <!-- body --> | ||
| Line 107: | Line 97: | ||
<a class="right-off-canvas-toggle mobile-menu" href="#"></a> | <a class="right-off-canvas-toggle mobile-menu" href="#"></a> | ||
<ul class="nav-utility inline-list"> | <ul class="nav-utility inline-list"> | ||
| − | <li class="nav-utility__item" | + | <li class="nav-utility__item" style="height:16px;"> </li></ul> |
| − | + | ||
| − | style="height:16px;"> </li></ul> | + | |
<nav class="nav-global" role="navigation"> | <nav class="nav-global" role="navigation"> | ||
<ul class="nav-global__list inline-list"> | <ul class="nav-global__list inline-list"> | ||
| Line 115: | Line 103: | ||
<a class="nav-global__link" title="" href="#">TEAM</a></li> | <a class="nav-global__link" title="" href="#">TEAM</a></li> | ||
<li class="nav-global__item trigger "> | <li class="nav-global__item trigger "> | ||
| − | <a class="nav-global__link" title="" | + | <a class="nav-global__link" title="" href="#">PROJECT</a></li> |
| − | + | ||
| − | href="#">PROJECT</a></li> | + | |
<li class="nav-global__item trigger "> | <li class="nav-global__item trigger "> | ||
| − | <a class="nav-global__link" title="" | + | <a class="nav-global__link" title="" href="#">PARTS</a></li> |
| − | + | ||
| − | href="#">PARTS</a></li> | + | |
<li class="nav-global__item trigger "> | <li class="nav-global__item trigger "> | ||
| − | <a class="nav-global__link" title="" | + | <a class="nav-global__link" title="" href="/Team:NUDT_CHINA/Safety">SAFETY</a></li> |
| − | + | ||
| − | href="/Team:NUDT_CHINA/Safety">SAFETY</a></li> | + | |
<li class="nav-global__item trigger "> | <li class="nav-global__item trigger "> | ||
| − | <a class="nav-global__link" title="" | + | <a class="nav-global__link" title="" href="/Team:NUDT_CHINA/Attributions">ATTRIBUTIONS</a></li> |
| − | + | ||
| − | href="/Team:NUDT_CHINA/Attributions">ATTRIBUTIONS</a></li> | + | |
<li class="nav-global__item trigger "> | <li class="nav-global__item trigger "> | ||
| − | <a class="nav-global__link" title="" | + | <a class="nav-global__link" title="" href="#">HUMAN<br/>PRACTICES</a></li> |
| − | + | ||
| − | href="#">HUMAN<br/>PRACTICES</a></li> | + | |
<li class="nav-global__item trigger "> | <li class="nav-global__item trigger "> | ||
| − | <a class="nav-global__link" title="" | + | <a class="nav-global__link" title="" href="#">AWARDS</a></li> |
| − | + | ||
| − | href="#">AWARDS</a></li> | + | |
</ul> | </ul> | ||
<style> | <style> | ||
| Line 145: | Line 121: | ||
#menu06{width:200px;right:0px;} #menu07{width:200px;right:0px;} | #menu06{width:200px;right:0px;} #menu07{width:200px;right:0px;} | ||
#menu01 .arrow-up{margin-right:6.429rem;} | #menu01 .arrow-up{margin-right:6.429rem;} | ||
| − | #menu02 .arrow-up{margin-right:90px;} #menu03 | + | #menu02 .arrow-up{margin-right:90px;} #menu03 .arrow-up{margin-right:90px;} #menu06 .arrow-up{margin-right:120px;} #menu07 .arrow-up{margin-right:27px;} #menu01{width:200px;right:31rem;} #menu02{width:200px;right:27rem;} #menu03{width:200px;right:21.3rem;} |
| − | + | ||
| − | .arrow-up{margin-right:90px;} #menu06 .arrow-up{margin-right:120px;} #menu07 | + | |
| − | + | ||
| − | .arrow-up{margin-right:27px;} #menu01{width:200px;right:31rem;} #menu02 | + | |
| − | + | ||
| − | {width:200px;right:27rem;} #menu03{width:200px;right:21.3rem;} | + | |
@media screen and (max-width: 64em) { | @media screen and (max-width: 64em) { | ||
#menu01{right:31.3rem;} | #menu01{right:31.3rem;} | ||
#menu02{right:27rem;} | #menu02{right:27rem;} | ||
| − | #menu03{right:21.8rem;} #menu06 .arrow-up | + | #menu03{right:21.8rem;} #menu06 .arrow-up{margin-right:130px;} #menu07 .arrow-up{margin-right:37px;} }</style> |
| − | + | ||
| − | {margin-right:130px;} #menu07 .arrow-up{margin-right:37px;} }</style> | + | |
<div class="content support" id="menu01"> | <div class="content support" id="menu01"> | ||
<div class="arrow-up"></div> | <div class="arrow-up"></div> | ||
| Line 167: | Line 135: | ||
<a href="/Team:NUDT_CHINA/Team">Team</a></li> | <a href="/Team:NUDT_CHINA/Team">Team</a></li> | ||
<li> | <li> | ||
| − | <a | + | <a href="/Team:NUDT_CHINA/Collaborations">Collaborations</a></li> |
| − | + | ||
| − | href="/Team:NUDT_CHINA/Collaborations">Collaborations</a></li> | + | |
</ul> | </ul> | ||
</nav> | </nav> | ||
| Line 180: | Line 146: | ||
<ul> | <ul> | ||
<li> | <li> | ||
| − | <a | + | <a href="/Team:NUDT_CHINA/Description">Description</a></li> |
| − | + | ||
| − | href="/Team:NUDT_CHINA/Description">Description</a></li> | + | |
<li> | <li> | ||
<a href="/Team:NUDT_CHINA/Design">Design</a></li> | <a href="/Team:NUDT_CHINA/Design">Design</a></li> | ||
<li> | <li> | ||
| − | <a | + | <a href="/Team:NUDT_CHINA/Experiments">Experiments</a></li> |
| − | + | ||
| − | href="/Team:NUDT_CHINA/Experiments">Experiments</a></li> | + | |
<li> | <li> | ||
| − | <a href="/Team:NUDT_CHINA/Proof">Proof of | + | <a href="/Team:NUDT_CHINA/Proof">Proof of Concept</a></li> |
| − | + | ||
| − | Concept</a></li> | + | |
<li> | <li> | ||
| − | <a | + | <a href="/Team:NUDT_CHINA/Demonstrate">Demonstrate</a></li> |
| − | + | ||
| − | href="/Team:NUDT_CHINA/Demonstrate">Demonstrate</a></li> | + | |
<li> | <li> | ||
<a href="/Team:NUDT_CHINA/Results">Results</a></li> | <a href="/Team:NUDT_CHINA/Results">Results</a></li> | ||
| Line 205: | Line 163: | ||
</div> | </div> | ||
<div class="column-2"> | <div class="column-2"> | ||
| − | <section id="c1560" class="text-and-images ce-margin-top-0 | + | <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> |
| − | + | ||
| − | ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> | + | |
<div class="tai__image-group-wrap large "> | <div class="tai__image-group-wrap large "> | ||
<div class="tai__image-group single-image"> | <div class="tai__image-group single-image"> | ||
| − | <!--a href="https#/support/donate/annual- | + | <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child"> |
| − | + | ||
| − | fund/donate-now/" class="lazyload-child"> | + | |
<img src="" /></a--> | <img src="" /></a--> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="ce-content rte-content rte-text-medium | + | <div class="ce-content rte-content rte-text-medium "></div> |
| − | + | ||
| − | "></div> | + | |
</section> | </section> | ||
</div> | </div> | ||
| Line 230: | Line 182: | ||
<a href="/Team:NUDT_CHINA/Parts">Parts</a></li> | <a href="/Team:NUDT_CHINA/Parts">Parts</a></li> | ||
<li> | <li> | ||
| − | <a href="/Team:NUDT_CHINA/Basic_Part">Basic | + | <a href="/Team:NUDT_CHINA/Basic_Part">Basic Parts</a></li> |
| − | + | ||
| − | Parts</a></li> | + | |
<li> | <li> | ||
| − | <a href="/Team:NUDT_CHINA/Composite_Part">Composite | + | <a href="/Team:NUDT_CHINA/Composite_Part">Composite Parts</a></li> |
| − | + | ||
| − | Parts</a></li> | + | |
<li> | <li> | ||
| − | <a href="/Team:NUDT_CHINA/Part_Collection">Part | + | <a href="/Team:NUDT_CHINA/Part_Collection">Part Collection</a></li> |
| − | + | ||
| − | Collection</a></li> | + | |
</ul> | </ul> | ||
</nav> | </nav> | ||
</div> | </div> | ||
<div class="column-2"> | <div class="column-2"> | ||
| − | <section id="c1560" class="text-and-images ce-margin-top-0 | + | <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> |
| − | + | ||
| − | ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> | + | |
<div class="tai__image-group-wrap large "> | <div class="tai__image-group-wrap large "> | ||
<div class="tai__image-group single-image"> | <div class="tai__image-group single-image"> | ||
| − | <!--a href="https#/support/donate/annual- | + | <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child"> |
| − | + | ||
| − | fund/donate-now/" class="lazyload-child"> | + | |
<img src="" /></a--> | <img src="" /></a--> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="ce-content rte-content rte-text-medium | + | <div class="ce-content rte-content rte-text-medium "></div> |
| − | + | ||
| − | "></div> | + | |
</section> | </section> | ||
</div> | </div> | ||
| Line 268: | Line 208: | ||
</div> | </div> | ||
<div class="column-2"> | <div class="column-2"> | ||
| − | <section id="c1560" class="text-and-images ce-margin-top-0 | + | <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> |
| − | + | ||
| − | ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> | + | |
<div class="tai__image-group-wrap large "> | <div class="tai__image-group-wrap large "> | ||
<div class="tai__image-group single-image"> | <div class="tai__image-group single-image"> | ||
| − | <!--a href="https#/support/donate/annual- | + | <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child"> |
| − | + | ||
| − | fund/donate-now/" class="lazyload-child"> | + | |
<img src="" /></a--> | <img src="" /></a--> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="ce-content rte-content rte-text-medium | + | <div class="ce-content rte-content rte-text-medium "></div> |
| − | + | ||
| − | "></div> | + | |
</section> | </section> | ||
</div> | </div> | ||
| Line 291: | Line 225: | ||
</div> | </div> | ||
<div class="column-2"> | <div class="column-2"> | ||
| − | <section id="c1560" class="text-and-images ce-margin-top-0 | + | <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> |
| − | + | ||
| − | ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> | + | |
<div class="tai__image-group-wrap large "> | <div class="tai__image-group-wrap large "> | ||
<div class="tai__image-group single-image"> | <div class="tai__image-group single-image"> | ||
| − | <!--a href="https#/support/donate/annual- | + | <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child"> |
| − | + | ||
| − | fund/donate-now/" class="lazyload-child"> | + | |
<img src="" /></a--> | <img src="" /></a--> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="ce-content rte-content rte-text-medium | + | <div class="ce-content rte-content rte-text-medium "></div> |
| − | + | ||
| − | "></div> | + | |
</section> | </section> | ||
</div> | </div> | ||
| Line 314: | Line 242: | ||
<ul> | <ul> | ||
<li> | <li> | ||
| − | <a href="/Team:NUDT_CHINA/Human_Practices">Human | + | <a href="/Team:NUDT_CHINA/Human_Practices">Human Practices</a></li> |
| − | + | ||
| − | Practices</a></li> | + | |
<li> | <li> | ||
<a href="/Team:NUDT_CHINA/HP/Silver">Silver</a></li> | <a href="/Team:NUDT_CHINA/HP/Silver">Silver</a></li> | ||
| Line 322: | Line 248: | ||
<a href="/Team:NUDT_CHINA/HP/Gold">Gold</a></li> | <a href="/Team:NUDT_CHINA/HP/Gold">Gold</a></li> | ||
<li> | <li> | ||
| − | <a | + | <a href="/Team:NUDT_CHINA/Integrated_Practices">Integrated Practices</a></li> |
| − | + | ||
| − | href="/Team:NUDT_CHINA/Integrated_Practices">Integrated Practices</a></li> | + | |
<li> | <li> | ||
| − | <a | + | <a href="/Team:NUDT_CHINA/Engagement">Engagement</a></li> |
| − | + | ||
| − | href="/Team:NUDT_CHINA/Engagement">Engagement</a></li> | + | |
</ul> | </ul> | ||
</nav> | </nav> | ||
</div> | </div> | ||
<div class="column-2"> | <div class="column-2"> | ||
| − | <section id="c1560" class="text-and-images ce-margin-top-0 | + | <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> |
| − | + | ||
| − | ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> | + | |
<div class="tai__image-group-wrap large "> | <div class="tai__image-group-wrap large "> | ||
<div class="tai__image-group single-image"> | <div class="tai__image-group single-image"> | ||
| − | <!--a href="https#/support/donate/annual- | + | <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child"> |
| − | + | ||
| − | fund/donate-now/" class="lazyload-child"> | + | |
<img src="" /></a--> | <img src="" /></a--> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="ce-content rte-content rte-text-medium | + | <div class="ce-content rte-content rte-text-medium "></div> |
| − | + | ||
| − | "></div> | + | |
</section> | </section> | ||
</div> | </div> | ||
| Line 361: | Line 277: | ||
</div> | </div> | ||
<div class="column-2"> | <div class="column-2"> | ||
| − | <section id="c1560" class="text-and-images ce-margin-top-0 | + | <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> |
| − | + | ||
| − | ce-margin-bottom-0 tai__ tai__image-center tai__image-above photo-teaser "> | + | |
<div class="tai__image-group-wrap large "> | <div class="tai__image-group-wrap large "> | ||
<div class="tai__image-group single-image"> | <div class="tai__image-group single-image"> | ||
| − | <!--a href="https#/support/donate/annual- | + | <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child"> |
| − | + | ||
| − | fund/donate-now/" class="lazyload-child"> | + | |
<img src="" /></a--> | <img src="" /></a--> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="ce-content rte-content rte-text-medium | + | <div class="ce-content rte-content rte-text-medium "></div> |
| − | + | ||
| − | "></div> | + | |
</section> | </section> | ||
</div> | </div> | ||
</div> | </div> | ||
</nav> | </nav> | ||
| − | <style>.nav-global ul li a{ font-size:0.85rem; } @media screen | + | <style>.nav-global ul li a{ font-size:0.85rem; } @media screen and (max-width: 865px) { .nav-global ul li a{ font-size:0.85rem; } } |
| − | + | ||
| − | and (max-width: 865px) { .nav-global ul li a{ font-size:0.85rem; } } | + | |
#feature{ | #feature{ | ||
| Line 396: | Line 304: | ||
<div class="breadcrumb"></div> | <div class="breadcrumb"></div> | ||
<!-- <div class="share"> | <!-- <div class="share"> | ||
| − | <a class="addthis_button_compact" tabindex="-1"><i class="icon | + | <a class="addthis_button_compact" tabindex="-1"><i class="icon icon-share"></i><span>Share</span></a> |
| − | + | ||
| − | icon-share"></i><span>Share</span></a> | + | |
<span></span></div> | <span></span></div> | ||
--> | --> | ||
| Line 406: | Line 312: | ||
<!-- end .row --></div> | <!-- end .row --></div> | ||
<!-- end #header-outer-wrap--> | <!-- end #header-outer-wrap--> | ||
| − | <div id="feature" class="row collapse text-light feature-footer-active" | + | <div id="feature" class="row collapse text-light feature-footer-active" style="background-color: rgb(3,13,38);background-image:url(https://static.igem.org/mediawiki/2016/thumb/3/3f/NUDT_CHINA2016_FILES-img-banner-3.0.png/800px-NUDT_CHINA2016_FILES-img-banner-3.0.png);background-position:0px -350px;background-repeat:no-repeat;"> |
| − | + | ||
| − | style="background-color: rgb(3,13,38);background-image:url | + | |
| − | + | ||
| − | (https://static.igem.org/mediawiki/2016/thumb/3/3f/NUDT_CHINA2016_FILES-img-banner- | + | |
| − | + | ||
| − | 3.0.png/800px-NUDT_CHINA2016_FILES-img-banner-3.0.png);background-position:0px | + | |
| − | + | ||
| − | -350px;background-repeat:no-repeat;"> | + | |
<div class="inner-wrap medium-24 columns"> | <div class="inner-wrap medium-24 columns"> | ||
<!--TYPO3SEARCH_begin--> | <!--TYPO3SEARCH_begin--> | ||
<div class="carousel-container hide-pager" id="c4811"> | <div class="carousel-container hide-pager" id="c4811"> | ||
| − | <div class="carousel " id="slideshow-4811" data-cycle-log="false" | + | <div class="carousel " id="slideshow-4811" data-cycle-log="false" data-cycle-auto-height="calc" data-cycle-prev="#prev-4811" data-cycle-next="#next-4811" data-cycle-pager="#cycle-pager-4811" data-cycle-easing="easeInOutQuad" data-cycle-fx="scrollVertUp" data-cycle-center-horz="true" data-cycle-speed="1000" data-cycle-timeout="8000" data-cycle-paused="true" data-cycle-slides="> div.slide"> |
| − | + | ||
| − | data-cycle-auto-height="calc" data-cycle-prev="#prev-4811" data-cycle- | + | |
| − | + | ||
| − | next="#next-4811" data-cycle-pager="#cycle-pager-4811" data-cycle- | + | |
| − | + | ||
| − | easing="easeInOutQuad" data-cycle-fx="scrollVertUp" data-cycle-center- | + | |
| − | + | ||
| − | horz="true" data-cycle-speed="1000" data-cycle-timeout="8000" data-cycle- | + | |
| − | + | ||
| − | paused="true" data-cycle-slides="> div.slide"> | + | |
<div class="slide first"> | <div class="slide first"> | ||
<link rel="stylesheet" href="/Team--NUDT_CHINA/CSS3.css" /> | <link rel="stylesheet" href="/Team--NUDT_CHINA/CSS3.css" /> | ||
<!-- Quick initialize with theme color from first slide --> | <!-- Quick initialize with theme color from first slide --> | ||
| − | <div class="row collapse " id="c6601" data-theme-class="bh- | + | <div class="row collapse " id="c6601" data-theme-class="bh-color__theme-c6601"> |
| − | + | ||
| − | color__theme-c6601"> | + | |
<div class="columns"> | <div class="columns"> | ||
| − | <section class="big-header bh__height-size-3 text-light | + | <section class="big-header bh__height-size-3 text-light bh__video" style="height:300px;"> |
| − | + | <div class="bh__background bh__background-color "></div> | |
| − | bh__video" style="height:300px;"> | + | |
| − | <div class="bh__background bh__background-color | + | |
| − | + | ||
| − | "></div> | + | |
<div class="bh__content-wrap" style="height:120px;"> | <div class="bh__content-wrap" style="height:120px;"> | ||
<div class="bh__content "> | <div class="bh__content "> | ||
<div class="bh__content-inner-wrap"> | <div class="bh__content-inner-wrap"> | ||
| − | <!--h3 class="bh__subtitle"><a | + | <!--h3 class="bh__subtitle"><a href="http#/explore/whats-here/exhibits/brick-by-brick/"><span class="line-wrap"><span class="line"><span class="bh__info">Exhibit / </span>Brick by Brick</span></span></a></h3--> |
| − | + | ||
| − | href="http#/explore/whats-here/exhibits/brick-by-brick/"><span class="line- | + | |
| − | + | ||
| − | wrap"><span class="line"><span class="bh__info">Exhibit / </span>Brick by | + | |
| − | + | ||
| − | Brick</span></span></a></h3--> | + | |
<h2 style="font-size:36px;" class="bh__title "> | <h2 style="font-size:36px;" class="bh__title "> | ||
<!--<a href="#">--> | <!--<a href="#">--> | ||
| − | <span style="line-height:46px;" class="line- | + | <span style="line-height:46px;" class="line-wrap"> |
| − | + | <span style="line-height:46px;" class="line">Development of A Novel</span></span> | |
| − | wrap"> | + | <span style="line-height:46px;" class="line-wrap"> |
| − | <span style="line-height:46px;" | + | <span style="line-height:46px;" class="line">Blood-MicroRNA Handy Detection System with CRISPR</span></span> |
| − | + | ||
| − | class="line">Development of A Novel</span></span> | + | |
| − | <span style="line-height:46px;" class="line- | + | |
| − | + | ||
| − | wrap"> | + | |
| − | <span style="line-height:46px;" | + | |
| − | + | ||
| − | class="line">Blood-MicroRNA | + | |
<!--</a>--></h2> | <!--</a>--></h2> | ||
</div> | </div> | ||
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<!-- end .columns --></div> | <!-- end .columns --></div> | ||
| − | <div style="margin-top:20px;" id="content-wrap-ink"><!-- adjust in add_JS | + | <div style="margin-top:20px;" id="content-wrap-ink"><!-- adjust in add_JS --> |
| − | + | ||
| − | --> | + | |
<div class="row footer-link" style=""> | <div class="row footer-link" style=""> | ||
<div style="text-align:right;"><h5> | <div style="text-align:right;"><h5> | ||
| − | <a style="color:rgb(10,31,84);" | + | <a style="color:rgb(10,31,84);" href="/Team:NUDT_CHINA">HomePage</a> • |
| + | <!-- 修改这里！！ -->AWARDS • | ||
| + | <a style="color:rgb(10,31,84);" href="/Team:NUDT_CHINA/Model"><!-- 修改这里！！ -->Model</a> | ||
| + | </h5><hr style="width:40%;margin-left:60%;border-top:1px solid rgb(10,31,84);" /></div> | ||
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| − | + | <!--标题--> | |
| − | + | ||
| − | + | ||
<h2> | <h2> | ||
<span><span style="color:#7f1015">Abstract</span></span><hr /> | <span><span style="color:#7f1015">Abstract</span></span><hr /> | ||
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model is created to evaluate the effectiveness of initial design, and offers | model is created to evaluate the effectiveness of initial design, and offers | ||
| − | guidelines | + | guidelines on how the system can (or must) be improved. (You can go to <a href="/Team:NUDT_CHINA/Design">PROJECT. page</a> to see more.) The core idea is to simulate the process of producing the signal which can be detected, and draw a conclusion by obtaining the relationship between the signal intensity and the concentration of miRNA. |
| − | + | ||
| − | href="/Team:NUDT_CHINA/Design"> PROJECT.page </a>to see more.) The core idea is | + | |
| − | + | ||
| − | to simulate the process of producing the | + | |
| − | + | ||
| − | signal which can be detected, and draw a conclusion by obtaining the | + | |
| − | + | ||
| − | relationship between the signal intensity and the concentration of miRNA. | + | |
| − | + | ||
</span> | </span> | ||
</p> | </p> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
| − | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The RCA |
| − | + | ||
| − | RCA | + | |
model is based on the Michaelis-Menten equation. We can obtain the relationship | model is based on the Michaelis-Menten equation. We can obtain the relationship | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">In the | <span style="line-height:2;font-family:Perpetua;font-size:18px;">In the | ||
| − | signal detection model, we combine a probability model with the kinetic | + | signal detection model, we combine a probability model with the kinetic equation |
| − | + | ||
| − | equation | + | |
of enzymatic reaction, so we can obtain the relationship between the number of | of enzymatic reaction, so we can obtain the relationship between the number of | ||
| Line 598: | Line 446: | ||
integrating the two models, we can theoretically predict the impacts of the | integrating the two models, we can theoretically predict the impacts of the | ||
| − | molecule number of proteins on the signal to noise ratio and | + | molecule number of proteins on the signal to noise ratio and explain the trend |
| − | + | of the signal intensity variation against the change of the concentration of miRNA in our | |
| − | + | ||
| − | of the signal intensity | + | |
wet-lab work. | wet-lab work. | ||
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<!--标题--> | <!--标题--> | ||
<h3> | <h3> | ||
| − | <span><span style="color:#7f1015"> About model </span></span | + | <span><span style="color:#7f1015"> About model </span></span> |
</h3> | </h3> | ||
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<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
| − | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1. MiRNA is not degraded throughout the reaction process.</span> |
| − | + | ||
| − | + | ||
</p> | </p> | ||
</br> | </br> | ||
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<!--正文--> | <!--正文--> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
| − | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2. The two fusion proteins of dCas9 and split-HRP fragments have the |
| − | + | ||
| − | + | ||
same ability to combine with the stem-loop structure, and only when two | same ability to combine with the stem-loop structure, and only when two | ||
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<!--正文--> | <!--正文--> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
| − | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">3. The number of stem-loop structures in each RCA product is equal |
| − | + | ||
| − | + | ||
under a certain reaction time. </span> | under a certain reaction time. </span> | ||
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<!--正文--> | <!--正文--> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
| − | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">4. The enzymatic activity remains unchanged with time under the premise |
| − | + | ||
| − | + | ||
| − | + | ||
| − | premise | + | |
of excessive amount of enzymes or a short-time reaction.</span> | of excessive amount of enzymes or a short-time reaction.</span> | ||
| Line 674: | Line 510: | ||
<!--标题--> | <!--标题--> | ||
<h3> | <h3> | ||
| − | <span><span style="color:#7f1015"> About the data </span></span | + | <span><span style="color:#7f1015"> About the data </span></span> |
| − | + | ||
| − | + | ||
</h3> | </h3> | ||
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<!--正文--> | <!--正文--> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
| − | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1. The data we obtain from wet-lab experiment are reliable.</span> |
| − | + | ||
| − | + | ||
</p> | </p> | ||
</br> | </br> | ||
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<!--正文--> | <!--正文--> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
| − | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2. All the results are trustworthy in the process of statistical |
| − | + | ||
| − | + | ||
processing and data calculation.</span> | processing and data calculation.</span> | ||
| Line 707: | Line 537: | ||
<!--标题--> | <!--标题--> | ||
<h3> | <h3> | ||
| − | <span><span style="color:#7f1015">Notations </span></span | + | <span><span style="color:#7f1015">Notations </span></span> |
</h3> | </h3> | ||
| Line 713: | Line 543: | ||
<!--表格--> | <!--表格--> | ||
| − | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" | + | <p> |
| − | + | <table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" style="border:none;"> | |
| − | style="border:none;"> | + | <tbody> |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">Symbol </span> | |
| − | windowtext 1.0pt;"> | + | </p> |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">Definition </span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | windowtext 1.0pt;"> | + | </tr> |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">x</span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | windowtext 1.0pt;"> | + | <a name="OLE_LINK7"></a><a name="OLE_LINK6"></a><span style="font-family:"">The |
| − | + | concentration of</span><span style="font-family:""> miRNA (pM)</span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | windowtext 1.0pt;"> | + | <p class="MsoNormal"> |
| − | + | <i><span style="font-family:"">C<sub>1</sub></span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | name="OLE_LINK6"></a><span>The concentration of</span><span> miRNA (pM)</span> | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">The | |
| − | + | concentration of initiated probe (Abbreviated to iprobe)</span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | windowtext 1.0pt;"> | + | <tr> |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">k<sub>1</sub></span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | windowtext 1.0pt;"> | + | <p class="MsoNormal"> |
| − | + | <a name="OLE_LINK8"></a><span style="font-family:"">A constant representing the scale factor</span><span style="font-family:""></span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | probe (Abbreviated | + | </tr> |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">K<sub>m </sub></span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | windowtext 1.0pt;"> | + | <p class="MsoNormal"> |
| − | + | <span style="font-family:"">One | |
| − | + | of the characteristic constants of phi29 DNA polymerase</span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | windowtext 1.0pt;"> | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">V<sub>max</sub></span></i> | |
| − | + | </p> | |
| − | constant | + | </td> |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | representing | + | <p class="MsoNormal"> |
| − | + | <span style="font-family:"">One | |
| − | + | of the characteristic constants of phi29 DNA polymerase</span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | windowtext 1.0pt;"> | + | <p class="MsoNormal"> |
| − | + | <i><span style="font-family:"">k<sub>2</sub></span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">A | |
| − | windowtext 1.0pt;"> | + | constant representing the scale factor</span> |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | constants of | + | <tr> |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">V</span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | windowtext 1.0pt;"> | + | <span style="font-family:"">The initial |
| − | + | speed of RCA </span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | windowtext 1.0pt;"> | + | <p class="MsoNormal"> |
| − | + | <a name="OLE_LINK9"></a><i><span style="font-family:"">n<sub>1</sub></span></i><i><sub><span style="font-family:""></span></sub></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | constants of phi29 | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">The | |
| − | + | moles of RCA product</span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | windowtext 1.0pt;"> | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">n<sub>2</sub></span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | windowtext 1.0pt;"> | + | <a name="OLE_LINK10"></a><span style="font-family:"">The number of stem-loop structures in each RCA product</span><span style="font-family:""></span> |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | factor</span> | + | <tr> |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">n</span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | windowtext 1.0pt;"> | + | <p class="MsoNormal"> |
| − | + | <span style="font-family:"">The total | |
| − | + | amount of <a name="OLE_LINK23"></a><a name="OLE_LINK22"></a>stem-loop structures</span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | windowtext 1.0pt;"> | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">N</span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <a name="OLE_LINK41"></a><a name="OLE_LINK16"></a><span style="font-family:"">The | |
| − | + | molecule number of the fusion protein of dCas9 and split-HRP fragments</span><span style="font-family:""></span> | |
| − | windowtext 1.0pt;"> | + | </p> |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | name="OLE_LINK9"></a><i><span>n<sub>1</sub></span></i><i><sub><span></span></ | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">k<sub>3</sub></span></i> | |
| − | + | </p> | |
| − | ></i> | + | </td> |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">A | |
| − | + | constant representing the scale factor</span> | |
| − | windowtext 1.0pt;"> | + | </p> |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">y<sub>1</sub></span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | windowtext 1.0pt;"> | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">The fluorescence | |
| − | + | intensity of DNA-dye-complex (RFU)</span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | windowtext 1.0pt;"> | + | <tr> |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">N<sub>1</sub></span></i> | |
| − | number of | + | </p> |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">The | |
| − | + | molecule number of the fusion protein of dCas9 and split-HRP fragments in the | |
| − | + | solution</span> | |
| − | + | </p> | |
| − | windowtext 1.0pt;"> | + | </td> |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">N<sub>2</sub></span></i> | |
| − | + | </p> | |
| − | windowtext 1.0pt;"> | + | </td> |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <a name="OLE_LINK45"></a><span style="font-family:"">The molecule number of the fusion protein of dCas9 and | |
| − | name="OLE_LINK23"></a><a name="OLE_LINK22"></a>stem-loop structures</span> | + | split-HRP fragments binding with stem-loop structure</span><span style="font-family:""></span> |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | windowtext 1.0pt;"> | + | <i><span style="font-family:"">k<sub>4</sub></span></i> |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">A | |
| − | + | constant representing the scale factor</span> | |
| − | windowtext 1.0pt;"> | + | </p> |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | name="OLE_LINK16"></a><span>The molecule number of the | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">k<sub>5</sub></span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">A | |
| − | windowtext 1.0pt;"> | + | constant representing the scale factor</span> |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | windowtext 1.0pt;"> | + | <i><span style="font-family:"">ρ</span></i><i><span style="font-family:""></span></i> |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | factor</span> | + | <p class="MsoNormal"> |
| − | + | <span style="font-family:"">Signal | |
| − | + | to noise ratio(Abbreviated to SNR)</span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | windowtext 1.0pt;"> | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">I</span></i> | |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | windowtext 1.0pt;"> | + | <span style="font-family:"">The |
| − | + | molecule number of formed intact HRP proteins </span> | |
| − | + | </p> | |
| − | DNA-dye-complex (RFU)</span> | + | </td> |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">I<sub>1</sub></span></i> | |
| − | + | </p> | |
| − | windowtext 1.0pt;"> | + | </td> |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">The | |
| − | + | molecule number of formed intact HRP proteins in the solution</span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | windowtext 1.0pt;"> | + | </tr> |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | protein | + | <i><span style="font-family:"">I<sub>2</sub></span></i> |
| − | + | </p> | |
| − | + | </td> | |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <a name="OLE_LINK43"></a><span style="font-family:"">The molecule number of formed intact HRP proteins through | |
| − | + | binding with stem-loop structure</span><span style="font-family:""></span> | |
| − | + | </p> | |
| − | windowtext 1.0pt;"> | + | </td> |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">t</span></i> | |
| − | + | </p> | |
| − | windowtext 1.0pt;"> | + | </td> |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">Reaction | |
| − | molecule number | + | time</span> |
| − | + | </p> | |
| − | + | </td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td width="66" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <i><span style="font-family:"">y<sub>2</sub></span></i> | |
| − | + | </p> | |
| − | windowtext 1.0pt;"> | + | </td> |
| − | + | <td width="487" valign="top" style="border:solid windowtext 1.0pt;"> | |
| − | + | <p class="MsoNormal"> | |
| − | + | <span style="font-family:"">The | |
| − | + | signal intensity (OD<sub>450</sub>)</span> | |
| − | + | </p> | |
| − | + | </td> | |
| − | windowtext 1.0pt;"> | + | </tr> |
| − | + | </tbody> | |
| − | + | </table> | |
| − | + | </p> | |
| − | factor</span> | + | |
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| − | + | ||
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| − | factor</span> | + | |
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| − | to SNR)</span> | + | |
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| − | intact HRP | + | |
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| − | intact HRP | + | |
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| − | molecule number | + | |
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| − | (OD<sub>450</sub>)</span> | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | </table> | + | |
| − | + | ||
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<!--标题--> | <!--标题--> | ||
<h3> | <h3> | ||
| − | <span><span style="color:#7f1015"> The RCA model </span></span | + | <span><span style="color:#7f1015"> The RCA model </span></span> |
| − | + | ||
| − | + | ||
</h3> | </h3> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">Next, | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Next, | ||
| − | RCA is an enzymatic reaction, the reaction equation is written as: iprobe+ | + | RCA is an enzymatic reaction, the reaction equation is written as: iprobe+dNTP→ |
| − | + | ||
| − | + | ||
iprobe-dN+PPi, under the premise of excessive amount of enzymes and dNTPs, the | iprobe-dN+PPi, under the premise of excessive amount of enzymes and dNTPs, the | ||
| Line 1,254: | Line 934: | ||
extending of each RCA production is related to the reaction time, not the | extending of each RCA production is related to the reaction time, not the | ||
| − | concentration of the iprobe. It is a linear relationship when we assume that | + | concentration of the iprobe. It is a linear relationship when we assume that the |
| − | + | ||
| − | the | + | |
enzymatic activity remains unchanged with time, thus, the number of stem-loop | enzymatic activity remains unchanged with time, thus, the number of stem-loop | ||
| Line 1,359: | Line 1,037: | ||
<span><span style="color:#7f1015"> The signal detection model | <span><span style="color:#7f1015"> The signal detection model | ||
| − | </span></span | + | </span></span> |
</h3> | </h3> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">The | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The | ||
| − | relationship between the number of Stem-loop structures and the signal | + | relationship between the number of Stem-loop structures and the signal intensity |
| − | + | ||
| − | intensity | + | |
under the premise of adding a certain amount of the fusion proteins can be | under the premise of adding a certain amount of the fusion proteins can be | ||
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<!--标题--> | <!--标题--> | ||
<h3> | <h3> | ||
| − | <span><span style="color:#7f1015"> The calculation of the constants | + | <span><span style="color:#7f1015"> The calculation of the constants |
| − | + | </span></span> | |
| − | + | ||
| − | </span></span | + | |
</h3> | </h3> | ||
| Line 1,603: | Line 1,277: | ||
which refers to the number of stem-loop structures | which refers to the number of stem-loop structures | ||
in each RCA product, is stable under the premise of a certain reaction time. | in each RCA product, is stable under the premise of a certain reaction time. | ||
| − | Thus, define two constants, mark as <i>a</i> and <i>b</i>, and then the | + | Thus, define two constants, mark as <i>a</i> and <i>b</i>, and then the equation |
| − | + | ||
| − | equation | + | |
can be | can be | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">We use | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We use | ||
| − | this equation to fit the data points obtained through experiments. (Figure 2. | + | this equation to fit the data points obtained through experiments. (Figure 2. |
| − | (D) in the<a href="/Team:NUDT_CHINA/Results"> | + | (D)in the <a href="/Team:NUDT_CHINA/Results">RESULTS.page</a>) The fitting |
curve is shown below.</span> | curve is shown below.</span> | ||
| Line 1,671: | Line 1,343: | ||
<span><span style="color:#7f1015"> Results and Analysis | <span><span style="color:#7f1015"> Results and Analysis | ||
| − | </span></span | + | </span></span> |
</h3> | </h3> | ||
| Line 1,708: | Line 1,380: | ||
intensity (OD<sub>450</sub>) | intensity (OD<sub>450</sub>) | ||
| − | against miRNA concentration(pM) and additional amount of fusion | + | against miRNA concentration(pM) and additional amount of fusion proteins.</span> |
| − | + | ||
| − | proteins.</span> | + | |
</p> | </p> | ||
</br> | </br> | ||
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<!--标题--> | <!--标题--> | ||
<h3> | <h3> | ||
| − | <span><span style="color:#7f1015">Test of model </span></span | + | <span><span style="color:#7f1015"> Test of model |
| + | |||
| + | </span></span> | ||
</h3> | </h3> | ||
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concentration of miRNA under the premise of adding a certain amount of the | concentration of miRNA under the premise of adding a certain amount of the | ||
| − | fusion proteins of dCas9 and split-HRP fragments. So we can test if our model | + | fusion proteins of dCas9 and split-HRP fragments. So we can test if our model is |
| − | + | reasonable by comparing the curves predicted by the model with those obtained | |
| − | + | from the experiment. The results are shown below. | |
| + | </span> | ||
</p> | </p> | ||
</br> | </br> | ||
| + | |||
| + | |||
<!--图和图注--> | <!--图和图注--> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
</br> | </br> | ||
</html> | </html> | ||
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<span style="line-height:2;font-family:Perpetua;font- | <span style="line-height:2;font-family:Perpetua;font- | ||
| − | size:18px;"><b>Figure 7. Test of model</b> | + | size:18px;"><b>Figure 7. Test of model</b> Plot of the signal intensity (OD<sub>450</sub>) against miRNA concentration (pM) predicted by the model and experimented by wet-lab. (A) The value of the molecule number of each fusion protein added to the system is set to be 4.66e+13. The mass of each fusion protein added to the system in wet-lab is 0.5 ng. (B) The value of the molecule number of each fusion protein added to the system is set to be 4.66e+14. The mass of each fusion protein added to the system in wet-lab is 5 ng.</span> |
| − | intensity (OD<sub>450</sub>) against miRNA concentration (pM) predicted by the | + | |
| − | model | + | |
| − | + | ||
| − | 4.66e+13. | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</p> | </p> | ||
| + | |||
</br> | </br> | ||
| + | |||
| + | |||
<!--正文--> | <!--正文--> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
| − | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We can learn | |
| − | + | from the figure above that the general trend of curves predicted by the model agree well with the experimental results. </span> | |
| − | + | ||
| − | + | ||
</p> | </p> | ||
</br> | </br> | ||
| + | |||
| + | |||
<!--标题--> | <!--标题--> | ||
<h3> | <h3> | ||
| − | <span><span style="color:#7f1015">Model extension </span></span | + | <span><span style="color:#7f1015"> Model extension |
| + | |||
| + | </span></span> | ||
</h3> | </h3> | ||
<!--正文--> | <!--正文--> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
| − | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Furthermore, | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Furthermore, we |
| − | + | consider that it is conducive to signal detection when the value of signal | |
intensity and SNR are both greater than two. We obtain the ideal region through | intensity and SNR are both greater than two. We obtain the ideal region through | ||
| − | calculation, which is shown as below. </span> | + | calculation, which is shown as below. </span> |
</p> | </p> | ||
</br> | </br> | ||
| + | |||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">When | <span style="line-height:2;font-family:Perpetua;font-size:18px;">When | ||
| − | the | + | the value of N is set to e+13.3, the range of the value of x contained in the |
| − | value of | + | |
| − | + | ideal region is a maximum, which means when the value of the molecule number of | |
| − | + | the fusion proteins is e+13.3, the concentration range of miRNA that can be | |
| − | + | ||
| − | miRNA that can be detected is maximum. </span> | + | detected is maximum. </span> |
</p> | </p> | ||
</br> | </br> | ||
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<!--正文--> | <!--正文--> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
| − | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The general trend of curves predicted by the model are in good agreement with the experimental |
| − | + | ||
| − | curves predicted by the model are in good agreement with the experimental | + | |
results, thus indicating that our model is reasonable.</span> | results, thus indicating that our model is reasonable.</span> | ||
| Line 1,922: | Line 1,582: | ||
<span style="line-height:2;font-family:Perpetua;font-size:18px;">The | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The | ||
| − | concentration range of miRNA that can be detected is maximum when we set the | + | concentration range of miRNA that can be detected is a maximum when we set the |
value of the molecule number of the fusion proteins to be e+13.3, building on | value of the molecule number of the fusion proteins to be e+13.3, building on | ||
| Line 1,938: | Line 1,598: | ||
<!--正文--> | <!--正文--> | ||
<p > | <p > | ||
| − | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1 Deng, R. <i>et al</i>. Toehold-initiated rolling | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1 Deng, R. <i>et al</i>.Toehold-initiated rolling circleamplification for visualizing individual microRNAs in situ in single cells. <i>Angew Chem Int Ed Engl </i>53, 2389-2393,doi:10.1002/anie.201309388 (2014).</span> |
</p> | </p> | ||
</br> | </br> | ||
| + | |||
| + | |||
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<div style="border:solid 1px rgb(10,31,84);background-color:rgb(10,31,84);"> | <div style="border:solid 1px rgb(10,31,84);background-color:rgb(10,31,84);"> | ||
<div class="row footer-link" style="border:solid 0px red;margin-top:100px;"> | <div class="row footer-link" style="border:solid 0px red;margin-top:100px;"> | ||
| − | <style>.menu-link-container ul li{ list-style:none; } .menu-link-container | + | <style>.menu-link-container ul li{ list-style:none; } .menu-link-container a{ color:white; font-size:14px;line-height:26px; } |
| − | + | ||
| − | a{ color:white; font-size:14px;line-height:26px; } | + | |
.menu-link-container a:hover{ | .menu-link-container a:hover{ | ||
text-decoration:underline; | text-decoration:underline; | ||
| − | } #c_menu9909 .medium-6{ text-align:center; width:auto; border:solid | + | } #c_menu9909 .medium-6{ text-align:center; width:auto; border:solid 0px red; } #c_menu9909 .medium-6 h5{color:white;}#c_menu9909 .medium-6{height:315px;border:solid 0px red;}</style> |
| − | + | ||
| − | 0px red; } #c_menu9909 .medium-6 h5{color:white;}#c_menu9909 .medium-6 | + | |
| − | + | ||
| − | {height:315px;border:solid 0px red;}</style> | + | |
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<a href="/Team:NUDT_CHINA/Basic_Part">Basic Parts</a><br/> | <a href="/Team:NUDT_CHINA/Basic_Part">Basic Parts</a><br/> | ||
| − | <a href="/Team:NUDT_CHINA/Composite_Part">Composite | + | <a href="/Team:NUDT_CHINA/Composite_Part">Composite Parts</a><br/> |
| − | + | ||
| − | Parts</a><br/> | + | |
| − | <a href="/Team:NUDT_CHINA/Part_Collection">Part | + | <a href="/Team:NUDT_CHINA/Part_Collection">Part Collection</a><br/> |
| − | + | ||
| − | Collection</a><br/> | + | |
</div> | </div> | ||
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| − | <A href="/Team:NUDT_CHINA/Attributions" style="text-decoration:none;"><h5 | + | <A href="/Team:NUDT_CHINA/Attributions" style="text-decoration:none;"><h5 class="column-header">ATTRIBUTIONS</h5></A> |
| − | + | ||
| − | class="column-header">ATTRIBUTIONS</h5></A> | + | |
<div class="menu-link-container"></div></div--> | <div class="menu-link-container"></div></div--> | ||
<div class="medium-6 columns" style="width:auto;"> | <div class="medium-6 columns" style="width:auto;"> | ||
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| − | <a href="/Team:NUDT_CHINA/Human_Practices">Human | + | <a href="/Team:NUDT_CHINA/Human_Practices">Human Practices</a><br/> |
| − | + | ||
| − | Practices</a><br/> | + | |
<a href="/Team:NUDT_CHINA/HP/Silver">Silver</a><br/> | <a href="/Team:NUDT_CHINA/HP/Silver">Silver</a><br/> | ||
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<a href="/Team:NUDT_CHINA/HP/Gold">Gold</a><br/> | <a href="/Team:NUDT_CHINA/HP/Gold">Gold</a><br/> | ||
| − | <a href="/Team:NUDT_CHINA/Integrated_Practices">Integrated | + | <a href="/Team:NUDT_CHINA/Integrated_Practices">Integrated Practices</a><br/> |
| − | + | ||
| − | Practices</a><br/> | + | |
<a href="/Team:NUDT_CHINA/Engagement">Engagement</a><br/> | <a href="/Team:NUDT_CHINA/Engagement">Engagement</a><br/> | ||
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<div class="row footer-link"> | <div class="row footer-link"> | ||
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| − | <img | + | <img src="https://static.igem.org/mediawiki/2016/thumb/2/22/NUDT_CHINA2016_FILES-img-igem-logo.png/800px-NUDT_CHINA2016_FILES-img-igem-logo.png" style="width:300px;"> |
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| − | igem-logo.png/800px-NUDT_CHINA2016_FILES-img-igem-logo.png" | + | |
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| − | style="width:300px;"> | + | |
<div class="content-open" style="width:300px;"> | <div class="content-open" style="width:300px;"> | ||
<p class="intro" style="font-size:18px;line-height:30px;"> | <p class="intro" style="font-size:18px;line-height:30px;"> | ||
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Latest revision as of 13:34, 19 October 2016
Abstract
This model is created to evaluate the effectiveness of initial design, and offers guidelines on how the system can (or must) be improved. (You can go to PROJECT. page to see more.) The core idea is to simulate the process of producing the signal which can be detected, and draw a conclusion by obtaining the relationship between the signal intensity and the concentration of miRNA.
Introduction
We create mathematical models of two aspects of our project, a RCA model and a signal detection model.
The RCA model is based on the Michaelis-Menten equation. We can obtain the relationship between the concentration of miRNA and the number of stem-loop structures through theoretical calculation, and we use our experimental results to calculate the parameters we introduced previously.
In the signal detection model, we combine a probability model with the kinetic equation of enzymatic reaction, so we can obtain the relationship between the number of stem-loop structures and the signal intensity under the premise of adding a certain amount of the fusion proteins of dCas9 and split-HRP fragments.
By integrating the two models, we can theoretically predict the impacts of the molecule number of proteins on the signal to noise ratio and explain the trend of the signal intensity variation against the change of the concentration of miRNA in our wet-lab work.
Assumption and Justification
About model
1. MiRNA is not degraded throughout the reaction process.
2. The two fusion proteins of dCas9 and split-HRP fragments have the same ability to combine with the stem-loop structure, and only when two different proteins next to each other, can they have the ability to catalyze substrate and produce signal.
3. The number of stem-loop structures in each RCA product is equal under a certain reaction time.
4. The enzymatic activity remains unchanged with time under the premise of excessive amount of enzymes or a short-time reaction.
About the data
1. The data we obtain from wet-lab experiment are reliable.
2. All the results are trustworthy in the process of statistical processing and data calculation.
Model
Notations
The RCA model
Firstly, we assume that there is a linear relationship between the concentration of the initiated probes and miRNA as the combination reaction between miRNA and probe occurs spontaneously1 . This can be written as:
Next, RCA is an enzymatic reaction, the reaction equation is written as: iprobe+dNTP→ iprobe-dN+PPi, under the premise of excessive amount of enzymes and dNTPs, the relationship between the concentration of iprobe and the initial speed of RCA can be described by the Michaelis-Menten equation. This can be written as：
It is obvious that there is a linear relationship between the molecule numbers of iprobe-dN (n1 , reflects the molecule numbers of RCA product) and the initial speed of RCA reaction. Notably, under the premise of excessive amount of enzymes, the extending of each RCA production is related to the reaction time, not the concentration of the iprobe. It is a linear relationship when we assume that the enzymatic activity remains unchanged with time, thus, the number of stem-loop structures (n2 ) in each RCA product is stable under the premise of a certain reaction time.
Figure 1. Schematic diagram
This can be written as:
And then, SYBR Green binds to DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 497nm) and emits green light (λmax = 520nm). Thus, there is a linear relationship between the total amount of stem-loop structures and the fluorescence intensity of DNA-dye-complex. This can be written as:
In summary, the relationship between the concentration of miRNA and the fluorescence intensity of DNA-dye-complex can be written as:
The signal detection model
Firstly, it is obvious that there is a linear relationship between the molecule number of formed intact HRP proteins in the solution (consider as NOISE) and the molecule number of the fusion proteins in the solution. This can be written as:
Next, we build a probability model to find the expression of the molecule number of formed intact HRP proteins (fused with dCas9) through binding with stem-loop structure (consider as SIGNAL). It is related to the total amount of stem-loop structures and the molecule number of DNA-bound fusion proteins. We obtain the result by Monte Carlo method.
Figure2. Frequency Distribution Plot of frequency distribution of the molecule number of formed intact HRP proteins through binding with stem-loop structure.
Figure 3. The result of the probability model Plot of the expect value of the molecule number of formed intact proteins against the total amount of stem-loop structures, carried out at 0.1, 0.3, 0.5, 0.7, 0.9 of N2 to n ratio.
The result can be written as:
As for N2, which refers to the molecule number of the fusion proteins binding with stem-loop structure. The equation is formulated based on the limiting case. The equation can be written as:
And then, we can obtain the expressions for ρ and I separately.
Notably, under the premise of excessive substrates, it is a linear relationship between the reaction rate and the concentration of enzymes when we assume that the enzymatic activity remain unchanged with time, thus, we can obtain the expression of the signal intensity at tth time-step. This is written as:
In summary, the relationship between the number of stem-loop structures and SNR under the premise of adding a certain amount of the fusion proteins can be written as:
The relationship between the number of Stem-loop structures and the signal intensity under the premise of adding a certain amount of the fusion proteins can be written as:
The calculation of the constants
To simplify the equation (1), the constants can be integrated. As can be seen from the above table, k1, k2, k3 are constants representing the scale factor, Km and Vmax are characteristic constants of phi 29 DNA polymerase, and n2, which refers to the number of stem-loop structures in each RCA product, is stable under the premise of a certain reaction time. Thus, define two constants, mark as a and b, and then the equation can be simplified as:
We use this equation to fit the data points obtained through experiments. (Figure 2. (D)in the RESULTS.page) The fitting curve is shown below.
Figure 4. Fitting curve Fitting curve of fluorescence intensity of DNA-dye-complex (RFU) against the concentrations of miRNA (pM).
With regard to the equation (2), we set the parameters based on the experiments as follows: k3 =8.35*10-16 (n=y1/k3); k4 =0.038; k5t=5.9*10-12.
Results and Analysis
By integrating the two models, we can obtain the relationships between SNR, the signal intensity respectively and the concentration of miRNA under different addition amount of fusion proteins.
The results are shown below.
Figure 5. The signal intensity (OD450) Three-dimensional map of signal intensity (OD450) against miRNA concentration(pM) and additional amount of fusion proteins.
There exists a monotonous relation between the concentration of miRNA and the signal intensity when the value of the molecule number of the fusion proteins is relatively large. While the relationship does not hold when the value of the molecule number of the fusion proteins is relatively small. And the signal intensity increases as the value of the molecule number of the fusion proteins increases.
Figure 6. The result of SNR Three-dimensional map of signal to noise ratio against miRNA concentration (pM) and additional amount of fusion proteins.
The signal-to-noise ratio decreases as the value of the molecule number of fusion proteins increases.
Test of model
Our model predicts the relationship between the signal intensity and the concentration of miRNA under the premise of adding a certain amount of the fusion proteins of dCas9 and split-HRP fragments. So we can test if our model is reasonable by comparing the curves predicted by the model with those obtained from the experiment. The results are shown below.
Figure 7. Test of model Plot of the signal intensity (OD450) against miRNA concentration (pM) predicted by the model and experimented by wet-lab. (A) The value of the molecule number of each fusion protein added to the system is set to be 4.66e+13. The mass of each fusion protein added to the system in wet-lab is 0.5 ng. (B) The value of the molecule number of each fusion protein added to the system is set to be 4.66e+14. The mass of each fusion protein added to the system in wet-lab is 5 ng.
We can learn from the figure above that the general trend of curves predicted by the model agree well with the experimental results.
Model extension
Furthermore, we consider that it is conducive to signal detection when the value of signal intensity and SNR are both greater than two. We obtain the ideal region through calculation, which is shown as below.
Figure 8. Ideal region Region of qualified logarithm of concentrations of miRNA (pM) and logarithm of the additional amount of fusion proteins.
When the value of N is set to e+13.3, the range of the value of x contained in the ideal region is a maximum, which means when the value of the molecule number of the fusion proteins is e+13.3, the concentration range of miRNA that can be detected is maximum.
Conclusion
Based on our simulation, we came to the conclusion that:
The general trend of curves predicted by the model are in good agreement with the experimental results, thus indicating that our model is reasonable.
The value of the molecule number of the fusion protein of dCas9 and split-HRP fragments not only affects the signal-to-noise ratio, but also the signal intensity. So we need to weigh its impact on both to select the optimal solution.
The concentration range of miRNA that can be detected is a maximum when we set the value of the molecule number of the fusion proteins to be e+13.3, building on which, our wet-lab protocol could be optimized in our future work.
Reference
1 Deng, R. et al.Toehold-initiated rolling circleamplification for visualizing individual microRNAs in situ in single cells. Angew Chem Int Ed Engl 53, 2389-2393,doi:10.1002/anie.201309388 (2014).
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