Difference between revisions of "Team:Tianjin/Parts"

 
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                           <header class="entry-header">
 
                           <header class="entry-header">
−
                <div class="entry-title" align="left" style="font-size:48px">Parts</div>
+
                <div class="entry-title" align="left" style="font-size:48px;margin-top:30px" >Parts</div>
 
                  </header><!-- .entry-header -->
 
                  </header><!-- .entry-header -->
−
                          <h1 class="entry-title">What We Did In This Summer</h1>
 
−
                       
 
  
  
−
                               <li>In this summer, we aimed at degrading <b>PET</b> biologically with two kinds of newly-found enzymes <b>PETase</b> and <b>MHETase</b>. </li>
+
                          <h1 class="entry-title">Parts For R-R System</h1>
−
  <li>We modified the enzyme <b>PETase</b> to improve its activity and used R-R (Reporting and Regulation) system to make the expression visible and regular. </li>
+
                        <table style="width:100%">
−
<li>We used microbial consortia to continue degrading the products <b>TPA</b> and <b>EG</b> to carbon dioxide. </li>
+
                            <tr style="width:100%">
−
<li>We also introduced Cyanobacteria to the microbial consortia so that the system can work with only optical energy and carbon dioxide. We also use <b>CFPS</b>(cell-free protein synthesis) system to assay the enzyme activity.</li>
+
                               <th style="width:20%;text-align:left"><b>Biobrick</b></th>
−
<li>In the Human Practice part, we spread out our project and the glamour of synthetic biology and receive financial help from some corporations.</li>
+
                              <th style="width:10%;text-align:left"><b>Type</b></th>
 +
                              <th style="width:60%;text-align:left"><b>Description</b></th>
 +
                              <th style="width:10%;text-align:left"><b>Length</b></th>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110000" target="_blank">BBa_K2110000</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">CYC1 Terminator</td>
 +
                              <td style="width:10%;text-align:left">248</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110012" target="_blank">BBa_K2110012</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PGK1 promoter</td>
 +
                              <td style="width:10%;text-align:left">781</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110017" target="_blank">BBa_K2110017</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">α-factor signal peptide</td>
 +
                              <td style="width:10%;text-align:left">267</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110001" target="_blank">BBa_K2110001</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">TPA inducible Leader sequence</td>
 +
                              <td style="width:10%;text-align:left">96</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110002" target="_blank">BBa_K2110002</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">TPA positive feedback regulatory gene</td>
 +
                              <td style="width:10%;text-align:left">807</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110003" target="_blank">BBa_K2110003</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">TPA transporting protein gene</td>
 +
                              <td style="width:10%;text-align:left">1380</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110004" target="_blank">BBa_K2110004</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">D-alanyl-D-alanine dipeptidase gene</td>
 +
                              <td style="width:10%;text-align:left">582</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110005" target="_blank">BBa_K2110005</a></td>
 +
                              <td style="width:10%;text-align:left">Composite</td>
 +
                              <td style="width:60%;text-align:left">TPA inducible RFP expression system</td>
 +
                              <td style="width:10%;text-align:left">2111</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110006" target="_blank">BBa_K2110006</a></td>
 +
                              <td style="width:10%;text-align:left">Composite</td>
 +
                              <td style="width:60%;text-align:left">TPA regulatory gene expression system</td>
 +
                              <td style="width:10%;text-align:left">1850</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110007" target="_blank">BBa_K2110007</a></td>
 +
                              <td style="width:10%;text-align:left">Composite</td>
 +
                              <td style="width:60%;text-align:left">TPA transporting protein gene expression system</td>
 +
                              <td style="width:10%;text-align:left">2425</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110008" target="_blank">BBa_K2110008</a></td>
 +
                              <td style="width:10%;text-align:left">Composite</td>
 +
                              <td style="width:60%;text-align:left">inclusion body induced cell lysis system</td>
 +
                              <td style="width:10%;text-align:left">665</td>
 +
                            </tr>
 +
                        </table>
  
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−
<h1 class="entry-title">General Support</h1>
+
<h1 class="entry-title">Parts For Microbial Consortium</h1>
−
<li>Our instructor <a href="http://qyjsyjy.tju.edu.cn:9080/FTRI/en_us/index.jsp" target="_blank"><b>Prof. Yingjin Yuan</b></a> and <b>Dr. Bingzhi Li</b>: A huge thanks for their constructive suggestions and meticulous guidance to our project.</li>
+
                        <table style="width:100%">
−
 
+
                            <tr style="width:100%">
 +
                              <th style="width:20%;text-align:left"><b>Biobrick</b></th>
 +
                              <th style="width:10%;text-align:left"><b>Type</b></th>
 +
                              <th style="width:60%;text-align:left"><b>Description</b></th>
 +
                              <th style="width:10%;text-align:left"><b>Length</b></th>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110009" target="_blank">BBa_K2110009</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">phage P22 holins</td>
 +
                              <td style="width:10%;text-align:left">327</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110010" target="_blank">BBa_K2110010</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">phage P22 auxiliary lysis factors</td>
 +
                              <td style="width:10%;text-align:left">438</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110011" target="_blank">BBa_K2110011</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">phage P22 endolysins</td>
 +
                              <td style="width:10%;text-align:left">441</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110013" target="_blank">BBa_K2110013</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase enzyme after codon optimization for bacillus subtilis</td>
 +
                              <td style="width:10%;text-align:left">885</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110014" target="_blank">BBa_K2110014</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">MHETase enzyme after codon optimization for bacillus subtilis</td>
 +
                              <td style="width:10%;text-align:left">1848</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110015" target="_blank">BBa_K2110015</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">The key gene for accumulation of PHA in P.putida KT2440</td>
 +
                              <td style="width:10%;text-align:left">978</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110016" target="_blank">BBa_K2110016</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">The key gene for utilization of ethylene glycolin P.putida KT2440</td>
 +
                              <td style="width:10%;text-align:left">1326</td>
 +
                            </tr>
 +
                        </table>
 
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−
<h1 class="entry-title">Protein modification Support</h1>
+
<h1 class="entry-title">Parts For Protein Modification</h1>
−
<li><b>Prof. Mingdong Yao</b>, who helped us to analyze the stability of PETase, gave many advices and discussed with us the practicability of our principles of designing mutations of PETase.</li>
+
                        <table style="width:100%">
−
<li><b>Dr. Yi Wu</b>, the man who generously shared restriction enzyme as well as watermelon, who gave us enlightening advices during showers, who got us a brand new microplate reader in the crisis, who helped novices to fix all kinds of experiment problems, and who supported the Tianjin iGEM team all the time.</li>
+
                            <tr style="width:100%">
−
<li><b>Dr. ZeXiong Xie</b>, who gave us advices and help on experiments all the time and supported Tianjin iGEM team.</li>
+
                              <th style="width:20%;text-align:left"><b>Biobrick</b></th>
 +
                              <th style="width:10%;text-align:left"><b>Type</b></th>
 +
                              <th style="width:60%;text-align:left"><b>Description</b></th>
 +
                              <th style="width:10%;text-align:left"><b>Length</b></th>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110800" target="_blank">BBa_K2110800</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase</td>
 +
                              <td style="width:10%;text-align:left">795</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110100" target="_blank">BBa_K2110100</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase site-directed mutant A240P</td>
 +
                              <td style="width:10%;text-align:left">795</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110101" target="_blank">BBa_K2110101</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase site-directed mutant S207T</td>
 +
                              <td style="width:10%;text-align:left">795</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110102" target="_blank">BBa_K2110102</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase site-directed mutant M154L</td>
 +
                              <td style="width:10%;text-align:left">795</td>
 +
                            </tr>
 +
                            <tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110103" target="_blank">BBa_K2110103</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase site-directed mutant N205V</td>
 +
                              <td style="width:10%;text-align:left">795</td>
 +
                            </tr>
 +
<tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110104" target="_blank">BBa_K2110104</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase site-directed mutant R90A</td>
 +
                              <td style="width:10%;text-align:left">795</td>
 +
                            </tr><tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110105" target="_blank">BBa_K2110105</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase site-directed mutant I208V</td>
 +
                              <td style="width:10%;text-align:left">795</td>
 +
                            </tr><tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110106" target="_blank">BBa_K2110106</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase site-directed mutant S238V</td>
 +
                              <td style="width:10%;text-align:left">795</td>
 +
                            </tr><tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110107" target="_blank">BBa_K2110107</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase site-directed mutant L117F</td>
 +
                              <td style="width:10%;text-align:left">795</td>
 +
                            </tr><tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110108" target="_blank">BBa_K2110108</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase site-directed mutant I208F</td>
 +
                              <td style="width:10%;text-align:left">795</td>
 +
                            </tr>
 +
<tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110109" target="_blank">BBa_K2110109</a></td>
 +
                              <td style="width:10%;text-align:left">Basic</td>
 +
                              <td style="width:60%;text-align:left">PETase site-directed mutant S238F</td>
 +
                              <td style="width:10%;text-align:left">795</td>
 +
                            </tr>
 +
<tr style="width:100%">
 +
                              <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110200" target="_blank">BBa_K2110200</a></td>
 +
                              <td style="width:10%;text-align:left">Composite</td>
 +
                              <td style="width:60%;text-align:left">PETase expression system in Saccharomyces cerevisiae</td>
 +
                              <td style="width:10%;text-align:left">1961</td>
 +
                            </tr>
 +
                        </table>
 
<hr>
 
<hr>
 +
<li><p style="font-size:25px"><b>See our sorted parts of <a href="https://2016.igem.org/Team:Tianjin/Basic_Part" target="_blank" style="color:#3399FF">Basic Parts</a>, <a href="https://2016.igem.org/Team:Tianjin/Composite_Part" target="_blank"style="color:#3399FF">Composite Parts</a> and <a href="https://2016.igem.org/Team:Tianjin/Part_Collection" target="_blank"style="color:#3399FF">Parts Collection</a>.</b></p></li>
  
 
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<h1 class="entry-title">Bacteria Consortium Support</h1>
 
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<li><b>Prof. CunJiang Song</b> generously sponsored us with P.putida KT2440 and the shuttle plasmid pBBR1MCS-2 which can be transformed into P.putida by electroporation.</li>
 
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<li><b>Prof. Aijun Miao</b> generously sponsored us with Rhodococcus sp. Strain RHA1.</li>
 
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<li><b>Prof. Rui Ban</b> generously sponsored us with Bacillus subtilis DB104.</li>
 
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<li><b>Prof. Tao Chen</b> generously sponsored us with Bacillus subtilis 168 and its available plasmids pH12-p43 and pH13-p43.</li>
 
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<li><b>Prof. Jun Yang</b> shared us with ideas about experimental design and measurement.</li>
 
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<h1 class="entry-title">Cyanobacteria Support</h1>
 
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<li><b>Prof. Weiwen Zhang</b> who allowed us to use their laboratories and equipments,  showed great patience when we occupied many of the facilities.</li>
 
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<li><b>Dr. Jinjin Diao</b>, at Tianjin University, helped us with his knowledge of Cyanobacterium Synechocystis sp. PCC 6803. He commented on our thoughts and ideas which helped us establishing the cell wall disruption process.</li>
 
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<h1 class="entry-title">CFPS(cell-free protein synthesis) System Support</h1>
 
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<li><b>Prof. Dayong Yang</b> Professor at Tianjin University. He commented on our thoughts about using the cell-free system to express our enzymes and ideas. He also offered us the  plasmid pRset_CFP-1 and allowed us to establish the expression system in his Lab.</li>
 
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<li><b>Yang Liu</b> postgraduate student in Prof. Dayong Yang’s Lab, who offered the material such as lysate of E.coli, feeding buffer and so on.</li>
 
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<li><b>Yannan Zhao</b> postgraduate student in Prof. Dayong Yang’s Lab, who assisted us to do some experiments and instucted us to use the ELIASA (microplate reader).</li>
 
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<h1 class="entry-title">Human Practice Support</h1>
 
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<li><b>GenScript</b> sponsors us with 60000 CNY.</li>
 
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<li><b>Synbio Technologies</b> sponsors us with 15000 CNY.</li>
 
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<li><a href="http://www.alltechms.com/" target="_blank"> <b>AllTech</b></a> sponsors us with 10000 CNY.</li>
 
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Latest revision as of 13:45, 19 October 2016

TEAM TIANJIN

LOGO


Team Tianjin-Attribution

Parts

Parts For R-R System

Biobrick Type Description Length
BBa_K2110000 Basic CYC1 Terminator 248
BBa_K2110012 Basic PGK1 promoter 781
BBa_K2110017 Basic α-factor signal peptide 267
BBa_K2110001 Basic TPA inducible Leader sequence 96
BBa_K2110002 Basic TPA positive feedback regulatory gene 807
BBa_K2110003 Basic TPA transporting protein gene 1380
BBa_K2110004 Basic D-alanyl-D-alanine dipeptidase gene 582
BBa_K2110005 Composite TPA inducible RFP expression system 2111
BBa_K2110006 Composite TPA regulatory gene expression system 1850
BBa_K2110007 Composite TPA transporting protein gene expression system 2425
BBa_K2110008 Composite inclusion body induced cell lysis system 665

Parts For Microbial Consortium

Biobrick Type Description Length
BBa_K2110009 Basic phage P22 holins 327
BBa_K2110010 Basic phage P22 auxiliary lysis factors 438
BBa_K2110011 Basic phage P22 endolysins 441
BBa_K2110013 Basic PETase enzyme after codon optimization for bacillus subtilis 885
BBa_K2110014 Basic MHETase enzyme after codon optimization for bacillus subtilis 1848
BBa_K2110015 Basic The key gene for accumulation of PHA in P.putida KT2440 978
BBa_K2110016 Basic The key gene for utilization of ethylene glycolin P.putida KT2440 1326

Parts For Protein Modification

Biobrick Type Description Length
BBa_K2110800 Basic PETase 795
BBa_K2110100 Basic PETase site-directed mutant A240P 795
BBa_K2110101 Basic PETase site-directed mutant S207T 795
BBa_K2110102 Basic PETase site-directed mutant M154L 795
BBa_K2110103 Basic PETase site-directed mutant N205V 795
BBa_K2110104 Basic PETase site-directed mutant R90A 795
BBa_K2110105 Basic PETase site-directed mutant I208V 795
BBa_K2110106 Basic PETase site-directed mutant S238V 795
BBa_K2110107 Basic PETase site-directed mutant L117F 795
BBa_K2110108 Basic PETase site-directed mutant I208F 795
BBa_K2110109 Basic PETase site-directed mutant S238F 795
BBa_K2110200 Composite PETase expression system in Saccharomyces cerevisiae 1961

  • See our sorted parts of Basic Parts, Composite Parts and Parts Collection.

    • Team Tianjin Sponsor Alltech
    Team Tianjin Sponsor GenScript
    Team Tianjin Sponsor SynbioTech