Difference between revisions of "Team:NUDT CHINA/Part Collection"
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">This part collection, namely PPI Tool-kit, | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This part collection, namely PPI Tool-kit, | ||
contained 18 new parts design for protein-protein interaction (PPI) researches.</span> | contained 18 new parts design for protein-protein interaction (PPI) researches.</span> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">In PPI Toolkit, we collected basic parts | <span style="line-height:2;font-family:Perpetua;font-size:18px;">In PPI Toolkit, we collected basic parts | ||
containing the coding sequence of three split-reporting systems: split-GFP, | containing the coding sequence of three split-reporting systems: split-GFP, | ||
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thus dramatically simplified the cloning process.</span> | thus dramatically simplified the cloning process.</span> | ||
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| + | <b><span style="line-height:2;font-family:Perpetua;font-size:18px;">Figure 1. Schematic representation of the workflow of the substitution system.</span></b> | ||
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<span style="line-height:2;font-family:Perpetua;font-size:18px;">All parts in this kit were functional | <span style="line-height:2;font-family:Perpetua;font-size:18px;">All parts in this kit were functional | ||
validated, well characterized and submitted to the registry.</span> | validated, well characterized and submitted to the registry.</span> | ||
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| − | <span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong></strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>Split-Luciferase based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong> | + | <span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong></strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>Split-Luciferase based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>chemiluminescence </strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>PPI Detector</strong></span> |
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Latest revision as of 22:52, 19 October 2016
HomePage • PARTS • Part Collection
Part Collection
Introduction
This part collection, namely PPI Tool-kit, contained 18 new parts design for protein-protein interaction (PPI) researches.
In PPI Toolkit, we collected basic parts containing the coding sequence of three split-reporting systems: split-GFP, split-Luciferase, and split-HRP. All those basic parts were codon-optimized to be expressed in E.coli, and sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining split-reporting system with a novel substitution system were designed, characterized and then added into the collection. Through which two proteins could be fused with their corresponding split-reporter fragment at the same time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning process.
Figure 1. Schematic representation of the workflow of the substitution system.
All parts in this kit were functional validated, well characterized and submitted to the registry.
Parts in our Collection
N-sGFP/ C-sGFP based fluorescent PPI Detector
| BBa_K1997004 | sGFP-N->Zif268->sGFP-C |
| BBa_K1997015 | P+R->sGFP-N->Zif268->sGFP-C->TER |
| BBa_K1997016 | P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER |
| BBa_K1997017 | P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER |
GFP10/ GFP11+GFP1-9 based fluorescent PPI Detector
| BBa_K1997014 | GFP10->Zif268->GFP11->RBS->GFP1-9 |
| BBa_K1997018 | P+R->GFP10->Zif268->GFP11->RBS->GFP1-9->TER |
| BBa_K1997019 | P+R->GFP10->Zif-N->RBS->Zif-C->GFP11->RBS->GFP1-9->TER |
| BBa_K1997020 | P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER |
Split-HRP based enzymatic PPI Detector
| BBa_K1997000 | sHRP-N |
| BBa_K1997001 | sHRP-C |
| BBa_K1997005 | sHRP-N->FRB->RBS->FKBP-> sHRP-C |
| BBa_K1997012 | P+R->sHRP-N->Zif268-> sHRP-C->TER |
| BBa_K1997013 | P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER |
Split-Luciferase based chemiluminescence PPI Detector
| BBa_K1997002 | sLuc-N |
| BBa_K1997003 | sLuc-C |
| BBa_K1997021 | P+R->sLuc-N->Zif268->sLuc-C->TER |
| BBa_K1997022 | P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER |
| BBa_K1997023 | P+R->sLuc-N->FRB->RBS->FKBP->sLuc-C->TER |
