Difference between revisions of "Team:NUDT CHINA/Part Collection"

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{{NUDT_CHINA}}
+
 
 
<html>
 
<html>
  
 +
  <head>
 +
<style>
 +
    #content{
 +
background-attachment: scroll;
 +
background-clip: border-box;
 +
background-color: rgb(255, 255, 255);
 +
background-image: none;
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background-origin: padding-box;
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background-repeat: repeat;
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background-size: auto auto;
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border-bottom-width: 0px;
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border-image-outset: 0 0 0 0;
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border-image-repeat: stretch stretch;
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border-image-width: 1 1 1 1;
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border-left-color: rgb(0, 0, 0);
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border-left-style: none;
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border-left-width: 0px;
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border-right-color: rgb(0, 0, 0);
 +
border-right-style: none;
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border-right-width: 0px;
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border-top-color: rgb(0, 0, 0);
 +
border-top-style: none;
 +
border-top-width: 0px;
 +
color: rgb(0, 0, 0);
 +
font-family: sans-serif;
 +
font-feature-settings: normal;
 +
font-kerning: auto;
 +
font-language-override: normal;
 +
font-size: 12.7px;
 +
font-size-adjust: none;
 +
font-stretch: normal;
 +
font-style: normal;
 +
font-synthesis: weight style;
 +
font-variant: normal;
 +
font-variant-alternates: normal;
 +
font-variant-caps: normal;
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font-variant-east-asian: normal;
 +
font-variant-ligatures: normal;
 +
font-variant-numeric: normal;
 +
font-variant-position: normal;
 +
font-weight: 400;
 +
line-height: 19.05px;
 +
margin:0px;
 +
margin-top: -5px;
 +
padding-bottom: 0px;
 +
padding-left: 0px;
 +
padding-right: 0px;
 +
padding-top: 0px;
 +
position: relative;
 +
width: 100%;
 +
z-index: 2;
 +
-moz-border-bottom-colors: none;
 +
-moz-border-left-colors: none;
 +
-moz-border-right-colors: none;
 +
-moz-border-top-colors: none;
  
 +
}
 +
    #top_title{display:none;}
 +
    #sideMenu{width:200px;display:none;}
 +
</style>
 +
  <meta charset="utf-8">
 +
    <meta name="HandheldFriendly" content="True" />
 +
    <meta name="MobileOptimized" content="320" />
 +
  <meta name="viewport" content="width=device-width, initial-scale=1.0",minimum-scale=0.2, maximum-scale=2.0, user-scalable=yes" />
 +
    <meta content="on" http-equiv="cleartype" />
 +
    <meta property="og:title" content="Home" />
 +
    <meta name="generator" content="TYPO3 CMS">
 +
    <link rel="stylesheet" type="text/css" href="/Team:NUDT_CHINA/CSS1?action=raw&ctype=text/css" media="all">
 +
    <title>NUDT_CHINA 2016</title>
 +
    <meta name="title" content="iGem - NUDT_CHINA 2016" />
 +
    <meta name="date" content="2016-10-04" />
 +
    <link rel="canonical" href="http#/" />
 +
    <!-- VhsAssetsDependenciesLoaded fontCss,mainCss,inlinejs-head,jquery,mainJs,cycle4811,cycle5332,cycle4823 -->
 +
    <link rel="stylesheet" href="/Team:NUDT_CHINA/CSS2?action=raw&ctype=text/css" />
 +
    <link rel="stylesheet" href="/Team:NUDT_CHINA/CSS?action=raw&ctype=text/css" /></head>
 +
  <!-- body -->
 +
 
 +
  <body id="f1a" class="home main bleed">
 +
    <div class="ribbon"></div>
 +
    <div class="off-canvas-wrap" data-offcanvas>
 +
      <div class="inner-wrap">
 +
        <!-- Off Canvas Menu -->
 +
        <!-- close the off-canvas menu -->
 +
        <a class="exit-off-canvas"></a>
 +
        <div id="header-outer-wrap" style="">
 +
          <header id="header" class="row">
 +
            <div class="medium-24 columns">
 +
              <a href="/Team:NUDT_CHINA">
 +
                <h1 class="site-id">Header...</h1></a>
 +
              <a class="right-off-canvas-toggle mobile-menu" href="#"></a>
 +
              <ul class="nav-utility inline-list">
 +
                <li class="nav-utility__item" style="height:16px;">&nbsp;</li></ul>
 +
              <nav class="nav-global" role="navigation">
 +
                <ul class="nav-global__list inline-list">
 +
                  <li class="nav-global__item trigger ">
 +
                    <a class="nav-global__link" title="" href="#">TEAM</a></li>
 +
                  <li class="nav-global__item trigger ">
 +
                    <a class="nav-global__link" title="" href="#">PROJECT</a></li>
 +
                  <li class="nav-global__item trigger ">
 +
                    <a class="nav-global__link" title="" href="#">PARTS</a></li>
 +
                  <li class="nav-global__item trigger ">
 +
                    <a class="nav-global__link" title="" href="/Team:NUDT_CHINA/Safety">SAFETY</a></li>
 +
                  <li class="nav-global__item trigger ">
 +
                    <a class="nav-global__link" title="" href="/Team:NUDT_CHINA/Attributions">ATTRIBUTIONS</a></li>
 +
                  <li class="nav-global__item trigger ">
 +
                    <a class="nav-global__link" title="" href="#">HUMAN<br/>PRACTICES</a></li>
 +
                  <li class="nav-global__item trigger ">
 +
                    <a class="nav-global__link" title="" href="#">AWARDS</a></li>
 +
                </ul>
 +
<style>
 +
.content{
 +
box-shadow: 6px 6px 3px #888888;
 +
}
 +
#menu06{width:200px;right:0px;} #menu07{width:200px;right:0px;}
 +
#menu01 .arrow-up{margin-right:6.429rem;}
 +
#menu02 .arrow-up{margin-right:90px;} #menu03 .arrow-up{margin-right:90px;} #menu06 .arrow-up{margin-right:120px;} #menu07 .arrow-up{margin-right:27px;} #menu01{width:200px;right:31rem;} #menu02{width:200px;right:27rem;} #menu03{width:200px;right:21.3rem;}
  
 +
@media screen and (max-width: 64em) {
 +
#menu01{right:31.3rem;}
 +
#menu02{right:27rem;}
 +
#menu03{right:21.8rem;} #menu06 .arrow-up{margin-right:130px;} #menu07 .arrow-up{margin-right:37px;} }</style>
 +
                <div class="content support" id="menu01">
 +
                  <div class="arrow-up"></div>
 +
                  <div class="column-1">
 +
                    <nav class="dropdown-arrow-list">
 +
                      <ul>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Team">Team</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Collaborations">Collaborations</a></li>
 +
                      </ul>
 +
                    </nav>
 +
                  </div>
 +
                </div>
 +
                <div class="content support" id="menu02">
 +
                  <div class="arrow-up"></div>
 +
                  <div class="column-1">
 +
                    <nav class="dropdown-arrow-list">
 +
                      <ul>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Description">Description</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Design">Design</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Experiments">Experiments</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Proof">Proof of Concept</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Demonstrate">Demonstrate</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Results">Results</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Notebook">Notebook</a></li>
 +
                      </ul>
 +
                    </nav>
 +
                  </div>
 +
                  <div class="column-2">
 +
                    <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above  photo-teaser ">
 +
                      <div class="tai__image-group-wrap large ">
 +
                        <div class="tai__image-group single-image">
 +
                          <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child">
 +
                            <img src="" /></a-->
 +
                        </div>
 +
                      </div>
 +
                      <div class="ce-content rte-content rte-text-medium "></div>
 +
                    </section>
 +
                  </div>
 +
                </div>
 +
                <div class="content support" id="menu03">
 +
                  <div class="arrow-up"></div>
 +
                  <div class="column-1">
 +
                    <nav class="dropdown-arrow-list">
 +
                      <ul>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Parts">Parts</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Basic_Part">Basic Parts</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Composite_Part">Composite Parts</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Part_Collection">Part Collection</a></li>
 +
                      </ul>
 +
                    </nav>
 +
                  </div>
 +
                  <div class="column-2">
 +
                    <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above  photo-teaser ">
 +
                      <div class="tai__image-group-wrap large ">
 +
                        <div class="tai__image-group single-image">
 +
                          <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child">
 +
                            <img src="" /></a-->
 +
                        </div>
 +
                      </div>
 +
                      <div class="ce-content rte-content rte-text-medium "></div>
 +
                    </section>
 +
                  </div>
 +
                </div>
 +
                <div class="content support" style="width:0px;height:0px;">
 +
                  <!--div class="arrow-up"></div-->
 +
                  <div class="column-1">
 +
                    <nav class="dropdown-arrow-list"></nav>
 +
                  </div>
 +
                  <div class="column-2">
 +
                    <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above  photo-teaser ">
 +
                      <div class="tai__image-group-wrap large ">
 +
                        <div class="tai__image-group single-image">
 +
                          <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child">
 +
                            <img src="" /></a-->
 +
                        </div>
 +
                      </div>
 +
                      <div class="ce-content rte-content rte-text-medium "></div>
 +
                    </section>
 +
                  </div>
 +
                </div>
 +
                <div class="content support" style="width:0px;height:0px;">
 +
                  <!--div class="arrow-up"></div-->
 +
                  <div class="column-1">
 +
                    <nav class="dropdown-arrow-list"></nav>
 +
                  </div>
 +
                  <div class="column-2">
 +
                    <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above  photo-teaser ">
 +
                      <div class="tai__image-group-wrap large ">
 +
                        <div class="tai__image-group single-image">
 +
                          <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child">
 +
                            <img src="" /></a-->
 +
                        </div>
 +
                      </div>
 +
                      <div class="ce-content rte-content rte-text-medium "></div>
 +
                    </section>
 +
                  </div>
 +
                </div>
 +
                <div class="content support" id="menu06">
 +
                  <div class="arrow-up"></div>
 +
                  <div class="column-1">
 +
                    <nav class="dropdown-arrow-list">
 +
                      <ul>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Human_Practices">Human Practices</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/HP/Silver">Silver</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/HP/Gold">Gold</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Integrated_Practices">Integrated Practices</a></li>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Engagement">Engagement</a></li>
 +
                      </ul>
 +
                    </nav>
 +
                  </div>
 +
                  <div class="column-2">
 +
                    <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above  photo-teaser ">
 +
                      <div class="tai__image-group-wrap large ">
 +
                        <div class="tai__image-group single-image">
 +
                          <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child">
 +
                            <img src="" /></a-->
 +
                        </div>
 +
                      </div>
 +
                      <div class="ce-content rte-content rte-text-medium "></div>
 +
                    </section>
 +
                  </div>
 +
                </div>
 +
                <div class="content support" id="menu07">
 +
                  <div class="arrow-up"></div>
 +
                  <div class="column-1">
 +
                    <nav class="dropdown-arrow-list">
 +
                      <ul>
 +
                        <li>
 +
                          <a href="/Team:NUDT_CHINA/Model">Model</a></li>
 +
                      </ul>
 +
                    </nav>
 +
                  </div>
 +
                  <div class="column-2">
 +
                    <section id="c1560" class="text-and-images ce-margin-top-0 ce-margin-bottom-0 tai__ tai__image-center tai__image-above  photo-teaser ">
 +
                      <div class="tai__image-group-wrap large ">
 +
                        <div class="tai__image-group single-image">
 +
                          <!--a href="https#/support/donate/annual-fund/donate-now/" class="lazyload-child">
 +
                            <img src="" /></a-->
 +
                        </div>
 +
                      </div>
 +
                      <div class="ce-content rte-content rte-text-medium "></div>
 +
                    </section>
 +
                  </div>
 +
                </div>
 +
</nav>
 +
              <style>.nav-global ul li a{ font-size:0.85rem; } @media screen and (max-width: 865px) { .nav-global ul li a{ font-size:0.85rem; } }
  
−
<div class="column full_size judges-will-not-evaluate">
+
#feature{
−
<h3>★  ALERT! </h3>
+
background-size:100%;
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">Part Collection special prize</a>. </p>
+
}
 +
  @media screen and (max-width: 1200px) {
 +
#feature{
 +
background-size:1200px;
 +
}
 +
  }  
 +
  </style>
  
 +
<div class="breadcrumb-share-wrap">
 +
                <div class="breadcrumb"></div>
 +
                <!-- <div class="share">
 +
                <a class="addthis_button_compact" tabindex="-1"><i class="icon icon-share"></i><span>Share</span></a>
 +
                <span></span></div>
 +
                -->
 +
              </div>
 +
            </div>
 +
            <!-- end .columns --></header>
 +
          <!-- end .row --></div>
 +
        <!-- end #header-outer-wrap-->
 +
        <div id="feature" class="row collapse text-light feature-footer-active" style="background-color: rgb(3,13,38);background-image:url(https://static.igem.org/mediawiki/2016/thumb/3/3f/NUDT_CHINA2016_FILES-img-banner-3.0.png/800px-NUDT_CHINA2016_FILES-img-banner-3.0.png);background-position:0px -350px;background-repeat:no-repeat;">
 +
          <div class="inner-wrap medium-24 columns">
 +
            <!--TYPO3SEARCH_begin-->
 +
            <div class="carousel-container hide-pager" id="c4811">
 +
              <div class="carousel " id="slideshow-4811" data-cycle-log="false" data-cycle-auto-height="calc" data-cycle-prev="#prev-4811" data-cycle-next="#next-4811" data-cycle-pager="#cycle-pager-4811" data-cycle-easing="easeInOutQuad" data-cycle-fx="scrollVertUp" data-cycle-center-horz="true" data-cycle-speed="1000" data-cycle-timeout="8000" data-cycle-paused="true" data-cycle-slides="> div.slide">
 +
                <div class="slide first">
 +
                  <link rel="stylesheet" href="/Team--NUDT_CHINA/CSS3.css" />
 +
                  <!-- Quick initialize with theme color from first slide -->
 +
                  <div class="row collapse " id="c6601" data-theme-class="bh-color__theme-c6601">
 +
                    <div class="columns">
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                      <section class="big-header bh__height-size-3 text-light bh__video" style="height:300px;">
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                        <div class="bh__background bh__background-color "></div>
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                        <div class="bh__content-wrap" style="height:120px;">
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                          <div class="bh__content ">
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                            <div class="bh__content-inner-wrap">
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                              <!--h3 class="bh__subtitle"><a href="http#/explore/whats-here/exhibits/brick-by-brick/"><span class="line-wrap"><span class="line"><span class="bh__info">Exhibit / </span>Brick by Brick</span></span></a></h3-->
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                              <h2 style="font-size:36px;" class="bh__title ">
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                                <!--<a href="#">-->
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                                <span style="line-height:46px;" class="line-wrap">
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                                  <span style="line-height:46px;" class="line">Development of A Novel</span></span>
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                                <span style="line-height:46px;" class="line-wrap">
 +
                                  <span style="line-height:46px;" class="line">Blood-MicroRNA Handy Detection System with CRISPR</span></span>
 +
                                <!--</a>--></h2>
 +
                            </div>
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                          </div>
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                        </div>
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      <div style="margin-top:20px;" id="content-wrap-ink"><!-- adjust in add_JS -->
 +
<div class="row footer-link" style="">
 +
<div style="text-align:right;"><h5>
 +
<a style="color:rgb(10,31,84);" href="/Team:NUDT_CHINA">HomePage</a> &bull;
 +
<!-- 修改这里！！ -->PARTS &bull;
 +
<a style="color:rgb(10,31,84);" href="/Team:NUDT_CHINA/Part_Collection"><!-- 修改这里！！ -->Part Collection</a>
 +
</h5><hr style="width:40%;margin-left:60%;border-top:1px solid rgb(10,31,84);" /></div>
  
  
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
 
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
 
  
 +
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<h2>
 +
<span><span style="color:#7f1015">Part Collection</span></span>
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</h2>
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</br>
 +
 +
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<h3>
 +
<span><span style="color:#7f1015">Introduction</span></span><hr />
 +
</h3>
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 +
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">This part collection, namely PPI Tool-kit,
 +
contained 18 new parts design for protein-protein interaction (PPI) researches.</span>
 +
</p>
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">In PPI Toolkit, we collected basic parts
 +
containing the coding sequence of three split-reporting systems: split-GFP,
 +
split-Luciferase, and split-HRP. All those basic parts were codon-optimized to
 +
be expressed in E.coli, and sequence-modified to meet the demands of RCF10.
 +
Building on that, amazing parts combining split-reporting system with a novel
 +
substitution system were designed, characterized and then added into the
 +
collection. Through which two proteins could be fused with their corresponding
 +
split-reporter fragment at the same time in one simple Golden-Gate Assembly,
 +
thus dramatically simplified the cloning process.</span>
 +
</p>
 +
</br>
 +
</html>
 +
[[File:T--NUDT_CHINA--collectionfig0.jpg|500px|center]]
 +
<html>
 
<p>
 
<p>
−
While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.  
+
<b><span style="line-height:2;font-family:Perpetua;font-size:18px;">Figure 1. Schematic representation of the workflow of the substitution system.</span></b>
 
</p>
 
</p>
  
 +
</br>
 +
 +
 +
<p style="text-indent:22pt;">
 +
<span style="line-height:2;font-family:Perpetua;font-size:18px;">All parts in this kit were functional
 +
validated, well characterized and submitted to the registry.</span>
 +
</p>
 +
<p>
 +
</br></br>
 +
</p>
 +
 +
 +
 +
<h3>
 +
<span><span style="color:#7f1015">Parts in our Collection</span></span><hr />
 +
</h3>
 +
 +
</br>
 +
<p>
 +
<span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>N-sGFP/ C-sGFP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>fluorescent </strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>PPI Detector</strong></span>
 +
</p>
 +
 +
</html>
 +
[[File:T--NUDT_CHINA--collectionfig1.jpg|500px|left]]
 +
<html>
 +
 +
 +
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997004" style = "color:#d82545;">BBa_K1997004</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;sGFP-N->Zif268->sGFP-C</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997015" style = "color:#d82545;">BBa_K1997015</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sGFP-N->Zif268->sGFP-C->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997016" style = "color:#d82545;">BBa_K1997016</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997017" style = "color:#d82545;">BBa_K1997017</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER</td>
 +
</table>
 +
</br></br>
 +
<p>
 +
<span style="line-height:2;font-family:Perpetua;font-size:25px;"></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>GFP10/ GFP11+GFP1-9 based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>fluorescent</strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong> PPI Detector</strong></span>
 +
</p>
 +
 +
</html>
 +
[[File:T--NUDT_CHINA--collectionfig2.jpg|500px|left]]
 +
<html>
 +
 +
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997014" style = "color:#d82545;">BBa_K1997014</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;GFP10->Zif268->GFP11->RBS->GFP1-9</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997018" style = "color:#d82545;">BBa_K1997018</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->GFP10->Zif268->GFP11->RBS->GFP1-9->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997019" style = "color:#d82545;">BBa_K1997019</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->GFP10->Zif-N->RBS->Zif-C->GFP11->RBS->GFP1-9->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997020" style = "color:#d82545;">BBa_K1997020</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER</td>
 +
</table>
 +
</br></br>
 +
<p>
 +
<span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong></strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>Split-HRP based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>enzymatic </strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>PPI Detector</strong></span>
 +
</p>
 +
 +
 +
</html>
 +
[[File:T--NUDT_CHINA--collectionfig3.jpg|500px|left]]
 +
<html>
 +
 +
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997000" style = "color:#d82545;">BBa_K1997000</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;sHRP-N</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997001" style = "color:#d82545;">BBa_K1997001</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;sHRP-C</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997005" style = "color:#d82545;">BBa_K1997005</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;sHRP-N->FRB->RBS->FKBP-> sHRP-C</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997012" style = "color:#d82545;">BBa_K1997012</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sHRP-N->Zif268-> sHRP-C->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997013" style = "color:#d82545;">BBa_K1997013</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER</td>
 +
</tr>
 +
</table>
 +
</br></br>
 +
<p>
 +
<span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong></strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>Split-Luciferase based </strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>chemiluminescence </strong></span><span style="line-height:2;font-family:Perpetua;font-size:25px;"><strong>PPI Detector</strong></span>
 +
</p>
 +
 +
</html>
 +
[[File:T--NUDT_CHINA--collectionfig4.jpg|500px|left]]
 +
<html>
 +
 +
<table style="line-height:2;font-family:Perpetua;font-size:18px;">
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997002" style = "color:#d82545;">BBa_K1997002</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;sLuc-N</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997003" style = "color:#d82545;">BBa_K1997003</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;sLuc-C</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997021" style = "color:#d82545;">BBa_K1997021</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sLuc-N->Zif268->sLuc-C->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997022" style = "color:#d82545;">BBa_K1997022</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER</td>
 +
</tr>
 +
<tr><td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1997023" style = "color:#d82545;">BBa_K1997023</a></td>
 +
<td>&nbsp;&nbsp;&nbsp;P+R->sLuc-N->FRB->RBS->FKBP->sLuc-C->TER</td>
 +
</tr>
 +
</table>
 +
</br>
 +
 +
 +
 +
 +
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</div>
 +
      </div>
 +
      <!-- end #content-wrap -->
 +
<div style="border:solid 1px rgb(10,31,84);background-color:rgb(10,31,84);">
 +
  <div class="row footer-link" style="border:solid 0px red;margin-top:100px;">
 +
    <style>.menu-link-container ul li{ list-style:none; } .menu-link-container a{ color:white; font-size:14px;line-height:26px; }
 +
.menu-link-container a:hover{
 +
text-decoration:underline;
 +
} #c_menu9909 .medium-6{ text-align:center; width:auto; border:solid 0px red; } #c_menu9909 .medium-6 h5{color:white;}#c_menu9909 .medium-6{height:315px;border:solid 0px red;}</style>
 +
    <div id="c_menu9909">
 +
      <!--div class="medium-6 columns" style="width:auto;">
 +
      <h5 class="column-header">HOME</h5>
 +
      <div class="menu-link-container"></div></div-->
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        <h5 class="column-header">TEAM</h5>
 +
        <div class="menu-link-container">
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              <a href="/Team:NUDT_CHINA/Team">Team</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Collaborations">Collaborations</a><br/>
 +
         
 +
        </div>
 +
      </div>
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      <div class="medium-6 columns" style="width:auto;">
 +
        <h5 class="column-header">PROJECT</h5>
 +
        <div class="menu-link-container">
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              <a href="/Team:NUDT_CHINA/Description">Description</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Design">Design</a><br/>
 +
           
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              <a href="/Team:NUDT_CHINA/Experiments">Experiments</a><br/>
 +
           
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              <a href="/Team:NUDT_CHINA/Proof">Proof of Concept</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Demonstrate">Demonstrate</a><br/>
 +
           
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              <a href="/Team:NUDT_CHINA/Results">Results</a><br/>
 +
           
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              <a href="/Team:NUDT_CHINA/Notebook">Notebook</a><br/>
 +
         
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        </div>
 +
      </div>
 +
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 +
        <h5 class="column-header">PARTS</h5>
 +
        <div class="menu-link-container">
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              <a href="/Team:NUDT_CHINA/Parts">Parts</a><br/>
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              <a href="/Team:NUDT_CHINA/Basic_Part">Basic Parts</a><br/>
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              <a href="/Team:NUDT_CHINA/Composite_Part">Composite Parts</a><br/>
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              <a href="/Team:NUDT_CHINA/Part_Collection">Part Collection</a><br/>
 +
         
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        </div>
 +
      </div>
 +
      <div class="medium-6 columns" style="width:auto;">
 +
        <a href="/Team:NUDT_CHINA/Safety" style="text-decoration:none;">
 +
          <h5 class="column-header">SAFETY</h5></a>
 +
       
 +
      </div>
 +
      <div class="medium-6 columns" style="width:auto;">
 +
        <a href="/Team:NUDT_CHINA/Attributions" style="text-decoration:none;">
 +
          <h5 class="column-header">ATTRIBUTIONS</h5></a>
 +
       
 +
      </div>  
 +
      <!--div class="medium-6 columns" style="width:auto;">
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      <A href="/Team:NUDT_CHINA/Attributions" style="text-decoration:none;"><h5 class="column-header">ATTRIBUTIONS</h5></A>
 +
      <div class="menu-link-container"></div></div-->
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      <div class="medium-6 columns" style="width:auto;">
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        <h5 class="column-header">HUMAN
 +
          <br>PRACTICES</h5>
 +
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              <a href="/Team:NUDT_CHINA/Human_Practices">Human Practices</a><br/>
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              <a href="/Team:NUDT_CHINA/HP/Silver">Silver</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/HP/Gold">Gold</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Integrated_Practices">Integrated Practices</a><br/>
 +
           
 +
              <a href="/Team:NUDT_CHINA/Engagement">Engagement</a><br/>
 +
         
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        </div>
 +
      </div>
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      <div class="medium-6 columns" style="width:auto;">
 +
        <h5 class="column-header">AWARDS</h5>
 +
        <div class="menu-link-container">
 +
         
 +
           
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              <a href="/Team:NUDT_CHINA/Model">Model</a><br/>
 +
         
 +
        </div>
 +
      </div>
 +
      <div class="medium-6 columns" style="width:0px;height:0px;"></div>
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    </div>
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  </div>
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</div>  
 +
      <footer id="footer">
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            <img src="https://static.igem.org/mediawiki/2016/thumb/2/22/NUDT_CHINA2016_FILES-img-igem-logo.png/800px-NUDT_CHINA2016_FILES-img-igem-logo.png" style="width:300px;">
 +
            <div class="content-open" style="width:300px;">
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              <p class="intro" style="font-size:18px;line-height:30px;">
 +
  <br/>No. 109 Deya Road, Kaifu District,
 +
<br/>Changsha, Hunan Province 410073
 +
<br/>P.R.China
 +
 +
                </p>
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                  <!--ul class="arrow-list footer-support-links">
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                  <li><a href="/support/membership/">Become a member</a></li>
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                  <li><a href="/support/donate/">Donate now</a></li></ul--></div>
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          </div>
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          <div style="position:relative;left:250px;top:10px;width:500px;"><h5 style="color:white;">SPONSORS</h5></div>
 +
  <div style="position:relative;left:0px;margin-left:600px;top:20px;">
 +
<img style="width:300px;" src="https://static.igem.org/mediawiki/2016/d/dc/T--NUDT_CHINA--Factory1.png" /><br/><br/>
 +
<img style="width:300px;" src="https://static.igem.org/mediawiki/2016/thumb/a/a2/T--NUDT_CHINA--Factory2.png/320px-T--NUDT_CHINA--Factory2.png" /><br/><br/>
 +
<img style="width:300px;" src="https://static.igem.org/mediawiki/2016/a/a3/T--NUDT_CHINA--Factory3.png" />
 +
  </div>
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          <!-- end .columns --></div>
 +
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Latest revision as of 22:52, 19 October 2016

NUDT_CHINA 2016

HomePage • PARTS • Part Collection

Part Collection


Introduction

This part collection, namely PPI Tool-kit, contained 18 new parts design for protein-protein interaction (PPI) researches.

In PPI Toolkit, we collected basic parts containing the coding sequence of three split-reporting systems: split-GFP, split-Luciferase, and split-HRP. All those basic parts were codon-optimized to be expressed in E.coli, and sequence-modified to meet the demands of RCF10. Building on that, amazing parts combining split-reporting system with a novel substitution system were designed, characterized and then added into the collection. Through which two proteins could be fused with their corresponding split-reporter fragment at the same time in one simple Golden-Gate Assembly, thus dramatically simplified the cloning process.


T--NUDT CHINA--collectionfig0.jpg

Figure 1. Schematic representation of the workflow of the substitution system.


All parts in this kit were functional validated, well characterized and submitted to the registry.



Parts in our Collection


N-sGFP/ C-sGFP based fluorescent PPI Detector

T--NUDT CHINA--collectionfig1.jpg

BBa_K1997004    sGFP-N->Zif268->sGFP-C
BBa_K1997015    P+R->sGFP-N->Zif268->sGFP-C->TER
BBa_K1997016    P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER
BBa_K1997017    P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER


GFP10/ GFP11+GFP1-9 based fluorescent PPI Detector

T--NUDT CHINA--collectionfig2.jpg

BBa_K1997014    GFP10->Zif268->GFP11->RBS->GFP1-9
BBa_K1997018    P+R->GFP10->Zif268->GFP11->RBS->GFP1-9->TER
BBa_K1997019    P+R->GFP10->Zif-N->RBS->Zif-C->GFP11->RBS->GFP1-9->TER
BBa_K1997020    P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER


Split-HRP based enzymatic PPI Detector

T--NUDT CHINA--collectionfig3.jpg

BBa_K1997000    sHRP-N
BBa_K1997001    sHRP-C
BBa_K1997005    sHRP-N->FRB->RBS->FKBP-> sHRP-C
BBa_K1997012    P+R->sHRP-N->Zif268-> sHRP-C->TER
BBa_K1997013    P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER


Split-Luciferase based chemiluminescence PPI Detector

T--NUDT CHINA--collectionfig4.jpg

BBa_K1997002    sLuc-N
BBa_K1997003    sLuc-C
BBa_K1997021    P+R->sLuc-N->Zif268->sLuc-C->TER
BBa_K1997022    P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER
BBa_K1997023    P+R->sLuc-N->FRB->RBS->FKBP->sLuc-C->TER


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