Difference between revisions of "Team:SVCE CHENNAI/Parts"

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<h1>PARTS</h1>
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<h1>Parts Collection</h1>
 
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<h2>Basic Parts</h2>
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Range: BBa_K2113001-BBa_K2113010.
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<h2>
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BASIC PARTS:
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    <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; Parts BBa_K2113004 and Part BBa_K2113005 are basic parts, coding for the antimicrobial peptides,the former without glycine and the later with glycine. A variant with extra glycine residues is used to increase the half life of the peptide, consistent with the N-end rule. </li>
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<h2>
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COMPOSITE PARTS:
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<li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; Parts BBa_K2113001 is a composite part coding for a RNA thermometer followed by expression of chromoprotein cj blue,this can be used for the characterization of RNA thermometer under different promoters. </li>
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    <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; Parts BBa_K2113002 is a composite part that serves as a temperature sensitive system with a lambda pr promoter under cI regulation. BBa_K2113003 similarly is a temperature sensitive system with a RNA thermometer.</li>
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     <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; Parts BBa_K2113007 and BBa_K2113008 are composite parts regulating the expression of basic parts BBa_K2113004 and BBa_K2113005 under a lac promoter, so that the system gets activated in the presence of milk.</li>
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     <li><span><i class="fa fa-caret-right" aria-hidden="true"></i></span>&nbsp;&nbsp; Part BBa_K2113006 contains a strong promoter regulating part BBa_k211301 downstream of which a double terminator is present. BBa_K2113009 and BBa_K2113010 are composite parts regulating the expression of basic parts BBa_K2113004 and BBa_K2113005 under a RNA thermometer.</li>          
 
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<p>To read more about the basic parts we submitted <a href="">click here</a></p>
 
 
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<h2>Cost of System for different sizes of Membrane</h2>
 
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     <th>Part Name</th>
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    <td>RNA thermometer + cjBlue</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2113001">BBa_K2113001 <i class="fa fa-external-link-square" aria-hidden="true"></i></a></td>      
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      <td>temperature sensitive system with lambda pr promoter under cI regulation</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2113002">BBa_K2113002 <i class="fa fa-external-link-square" aria-hidden="true"></i></a></td>
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      <td>temperature sensitive system with RNA thermometer</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2113003" target="blank_">BBa_K2113003 <i class="fa fa-external-link-square" aria-hidden="true"></i></a></td>
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      <td>AMP without glycine</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2113004" target="blank_">BBa_K2113004 <i class="fa fa-external-link-square" aria-hidden="true"></i></a></td>
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      <td>AMP with glycine</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2113005" target="blank_">BBa_K2113005 <i class="fa fa-external-link-square" aria-hidden="true"></i></a></td>
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  </tr>
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      <td>temperature sensitive system with RNA thermometer with cj blue</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2113006" target="blank_">BBa_K2113006 <i class="fa fa-external-link-square" aria-hidden="true"></i></a></td>
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      <td>lac inducible AMP without glycine</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2113007" target="blank_">BBa_K2113007 <i class="fa fa-external-link-square" aria-hidden="true"></i></a></td>
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      <td>lac inducible AMP with glycine</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2113008" target="blank_">BBa_K2113008 <i class="fa fa-external-link-square" aria-hidden="true"></i></a></td>
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      <td>RNA thermometer and AMP without glycine</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2113009" target="blank_">BBa_K2113009 <i class="fa fa-external-link-square" aria-hidden="true"></i></a></td>
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      <td>RNA thermometer and AMP with glycine</td>
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      <td><a href="http://parts.igem.org/Part:BBa_K2113010" target="blank_">BBa_K2113010 <i class="fa fa-external-link-square" aria-hidden="true"></i></a></td>
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Revision as of 23:55, 19 October 2016

Lactoshield - SVCE_CHENNAI Lactoshield - SVCE_CHENNAI

Lactoshield - SVCE_CHENNAI

Parts Collection

Range: BBa_K2113001-BBa_K2113010.

    BASIC PARTS:

  •    Parts BBa_K2113004 and Part BBa_K2113005 are basic parts, coding for the antimicrobial peptides,the former without glycine and the later with glycine. A variant with extra glycine residues is used to increase the half life of the peptide, consistent with the N-end rule.

    • COMPOSITE PARTS:

  •    Parts BBa_K2113001 is a composite part coding for a RNA thermometer followed by expression of chromoprotein cj blue,this can be used for the characterization of RNA thermometer under different promoters.
  •    Parts BBa_K2113002 is a composite part that serves as a temperature sensitive system with a lambda pr promoter under cI regulation. BBa_K2113003 similarly is a temperature sensitive system with a RNA thermometer.
  •    Parts BBa_K2113007 and BBa_K2113008 are composite parts regulating the expression of basic parts BBa_K2113004 and BBa_K2113005 under a lac promoter, so that the system gets activated in the presence of milk.
  •    Part BBa_K2113006 contains a strong promoter regulating part BBa_k211301 downstream of which a double terminator is present. BBa_K2113009 and BBa_K2113010 are composite parts regulating the expression of basic parts BBa_K2113004 and BBa_K2113005 under a RNA thermometer.

Cost of System for different sizes of Membrane

Part Name Part Number
RNA thermometer + cjBlue BBa_K2113001
temperature sensitive system with lambda pr promoter under cI regulation BBa_K2113002
temperature sensitive system with RNA thermometer BBa_K2113003
AMP without glycine BBa_K2113004
AMP with glycine BBa_K2113005
temperature sensitive system with RNA thermometer with cj blue BBa_K2113006
lac inducible AMP without glycine BBa_K2113007
lac inducible AMP with glycine BBa_K2113008
RNA thermometer and AMP without glycine BBa_K2113009
RNA thermometer and AMP with glycine BBa_K2113010