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− | + | <meta name="viewport" content="width=device-width, initial-scale=1.0",minimum-scale=0.2, maximum-scale=2.0, user-scalable=yes" /> | |
<meta content="on" http-equiv="cleartype" /> | <meta content="on" http-equiv="cleartype" /> | ||
<meta property="og:title" content="Home" /> | <meta property="og:title" content="Home" /> | ||
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<div class="inner-wrap"> | <div class="inner-wrap"> | ||
<!-- Off Canvas Menu --> | <!-- Off Canvas Menu --> | ||
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<a class="nav-global__link" title="" href="#">AWARDS</a></li> | <a class="nav-global__link" title="" href="#">AWARDS</a></li> | ||
</ul> | </ul> | ||
− | <style>#menu06{width:200px;right:0px;} #menu07{width:200px;right:0px;} | + | <style> |
+ | .content{ | ||
+ | box-shadow: 6px 6px 3px #888888; | ||
+ | } | ||
+ | #menu06{width:200px;right:0px;} #menu07{width:200px;right:0px;} | ||
#menu01 .arrow-up{margin-right:6.429rem;} | #menu01 .arrow-up{margin-right:6.429rem;} | ||
#menu02 .arrow-up{margin-right:90px;} #menu03 .arrow-up{margin-right:90px;} #menu06 .arrow-up{margin-right:120px;} #menu07 .arrow-up{margin-right:27px;} #menu01{width:200px;right:31rem;} #menu02{width:200px;right:27rem;} #menu03{width:200px;right:21.3rem;} | #menu02 .arrow-up{margin-right:90px;} #menu03 .arrow-up{margin-right:90px;} #menu06 .arrow-up{margin-right:120px;} #menu07 .arrow-up{margin-right:27px;} #menu01{width:200px;right:31rem;} #menu02{width:200px;right:27rem;} #menu03{width:200px;right:21.3rem;} | ||
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− | <style>.nav-global ul li a{ font-size:0.85rem; } @media screen and (max-width: 865px) { .nav-global ul li a{ font-size:0.85rem; } }</style> | + | <style>.nav-global ul li a{ font-size:0.85rem; } @media screen and (max-width: 865px) { .nav-global ul li a{ font-size:0.85rem; } } |
+ | |||
+ | #feature{ | ||
+ | background-size:100%; | ||
+ | } | ||
+ | @media screen and (max-width: 1200px) { | ||
+ | #feature{ | ||
+ | background-size:1200px; | ||
+ | } | ||
+ | } | ||
+ | </style> | ||
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− | <div id="feature" class="row collapse text-light feature-footer-active"> | + | <div id="feature" class="row collapse text-light feature-footer-active" style="background-color: rgb(3,13,38);background-image:url(https://static.igem.org/mediawiki/2016/thumb/3/3f/NUDT_CHINA2016_FILES-img-banner-3.0.png/800px-NUDT_CHINA2016_FILES-img-banner-3.0.png);background-position:0px -350px;background-repeat:no-repeat;"> |
<div class="inner-wrap medium-24 columns"> | <div class="inner-wrap medium-24 columns"> | ||
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<div class="carousel " id="slideshow-4811" data-cycle-log="false" data-cycle-auto-height="calc" data-cycle-prev="#prev-4811" data-cycle-next="#next-4811" data-cycle-pager="#cycle-pager-4811" data-cycle-easing="easeInOutQuad" data-cycle-fx="scrollVertUp" data-cycle-center-horz="true" data-cycle-speed="1000" data-cycle-timeout="8000" data-cycle-paused="true" data-cycle-slides="> div.slide"> | <div class="carousel " id="slideshow-4811" data-cycle-log="false" data-cycle-auto-height="calc" data-cycle-prev="#prev-4811" data-cycle-next="#next-4811" data-cycle-pager="#cycle-pager-4811" data-cycle-easing="easeInOutQuad" data-cycle-fx="scrollVertUp" data-cycle-center-horz="true" data-cycle-speed="1000" data-cycle-timeout="8000" data-cycle-paused="true" data-cycle-slides="> div.slide"> | ||
<div class="slide first"> | <div class="slide first"> | ||
− | <link rel="stylesheet" href="/Team | + | <link rel="stylesheet" href="/Team--NUDT_CHINA/CSS3.css" /> |
<!-- Quick initialize with theme color from first slide --> | <!-- Quick initialize with theme color from first slide --> | ||
<div class="row collapse " id="c6601" data-theme-class="bh-color__theme-c6601"> | <div class="row collapse " id="c6601" data-theme-class="bh-color__theme-c6601"> | ||
<div class="columns"> | <div class="columns"> | ||
− | <section class="big-header bh__height-size-3 text-light bh__video"> | + | <section class="big-header bh__height-size-3 text-light bh__video" style="height:300px;"> |
− | <div class="bh__background bh__background-color | + | <div class="bh__background bh__background-color "></div> |
− | <div class="bh__content-wrap"> | + | <div class="bh__content-wrap" style="height:120px;"> |
<div class="bh__content "> | <div class="bh__content "> | ||
<div class="bh__content-inner-wrap"> | <div class="bh__content-inner-wrap"> | ||
<!--h3 class="bh__subtitle"><a href="http#/explore/whats-here/exhibits/brick-by-brick/"><span class="line-wrap"><span class="line"><span class="bh__info">Exhibit / </span>Brick by Brick</span></span></a></h3--> | <!--h3 class="bh__subtitle"><a href="http#/explore/whats-here/exhibits/brick-by-brick/"><span class="line-wrap"><span class="line"><span class="bh__info">Exhibit / </span>Brick by Brick</span></span></a></h3--> | ||
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<h2 style="font-size:36px;" class="bh__title "> | <h2 style="font-size:36px;" class="bh__title "> | ||
<!--<a href="#">--> | <!--<a href="#">--> | ||
− | + | <span style="line-height:46px;" class="line-wrap"> | |
− | + | <span style="line-height:46px;" class="line">Development of A Novel</span></span> | |
− | + | <span style="line-height:46px;" class="line-wrap"> | |
− | + | <span style="line-height:46px;" class="line">Blood-MicroRNA Handy Detection System with CRISPR</span></span> | |
− | <!--</a>--> | + | <!--</a>--></h2> |
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="cycle-pager" id="cycle-pager-4811"></div> | <div class="cycle-pager" id="cycle-pager-4811"></div> | ||
</div> | </div> | ||
− | + | </div> | |
− | + | </div> | |
<!-- end #feature --> | <!-- end #feature --> | ||
<!-- end .columns --></div> | <!-- end .columns --></div> | ||
− | <div style="margin-top: | + | <div style="margin-top:20px;" id="content-wrap-ink"><!-- adjust in add_JS --> |
− | <div class="row footer-link" style=""> | + | <div class="row footer-link" style=""> |
+ | <div style="text-align:right;"><h5> | ||
+ | <a style="color:rgb(10,31,84);" href="/Team:NUDT_CHINA">HomePage</a> • | ||
+ | <!-- 修改这里!! -->Human Practice • | ||
+ | <a style="color:rgb(10,31,84);" href="/Team:NUDT_CHINA/Description"><!-- 修改这里!! -->Integrated Practices</a> | ||
+ | </h5><hr style="width:40%;margin-left:60%;border-top:1px solid rgb(10,31,84);" /></div> | ||
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− | < | + | |
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+ | |||
+ | |||
+ | |||
+ | |||
+ | <!--在这里插入内容--> | ||
+ | |||
<h2> | <h2> | ||
− | <span><span style="color:#7f1015"> | + | <span><span style="color:#7f1015">NUDT_CHINA’s Human Practice Tour</span></span><hr /> |
</h2> | </h2> | ||
+ | <h3> | ||
+ | <span><span style="color:#7f1015">MAP</span></span> | ||
+ | </h3> | ||
+ | |||
+ | </br> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <style> | ||
+ | .floatsci{ | ||
+ | border:solid 0px red; | ||
+ | background:url("https://static.igem.org/mediawiki/2016/c/cb/%E6%80%BB%E7%BB%93%E5%9B%BE2_NUDT_CHINA.jpg"); | ||
+ | background-size:700px 394px; | ||
+ | width:700px; | ||
+ | height:394px; | ||
+ | } | ||
+ | .floatsci div{ | ||
+ | border:solid 0px red; | ||
+ | position:relative; | ||
+ | cursor:pointer; | ||
+ | } | ||
+ | #div01_floatsci{ | ||
+ | left:-250px; | ||
+ | top:150px; | ||
+ | width:120px; | ||
+ | height:110px; | ||
+ | } | ||
+ | #div02_floatsci{ | ||
+ | left:110px; | ||
+ | top:-100px; | ||
+ | width:80px; | ||
+ | height:70px; | ||
+ | } | ||
+ | #div03_floatsci{ | ||
+ | left:226px; | ||
+ | top:-15px; | ||
+ | width:119px; | ||
+ | height:69px; | ||
+ | } | ||
+ | #div04_floatsci{ | ||
+ | left:50px; | ||
+ | top:70px; | ||
+ | width:110px; | ||
+ | height:55px; | ||
+ | } | ||
+ | |||
+ | </style> | ||
+ | <a id="a_href" href="/"> </a> | ||
+ | <script> | ||
+ | function jump_ink(url){ | ||
+ | var a_href=document.getElementById("a_href"); | ||
+ | a_href.href=url; | ||
+ | a_href.click(); | ||
+ | } | ||
+ | </script> | ||
+ | <div class="center"><div class="floatsci"> | ||
+ | |||
+ | <div id="div01_floatsci" onclick="jump_ink('https://2016.igem.org/Team:NUDT_CHINA/Results')"></div> | ||
+ | <div id="div02_floatsci" onclick="jump_ink('#PARTI')"></div> | ||
+ | <div id="div03_floatsci" onclick="jump_ink('#PARTII')"></div> | ||
+ | <div id="div04_floatsci" onclick="jump_ink('#PARTIII')"></div> | ||
+ | </div></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </br> | ||
+ | |||
+ | <p> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:16px;">(click figures to view details of our HP)</span> | ||
+ | </p> | ||
+ | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This year we expanded our project to Human Practice in three places: Hospital&Health Center, Market and the Public. In each expansion we held meaningful activities closely related to our topic, and this turned out to be a nice interaction between the project and the practice. </span> |
</p> | </p> | ||
</br> | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Arrows in the map show our practice work in two directions.</span> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Forward Arrows:What the project contributed to the world---a new medical application for Hospitals&Health Centers, a potential handy, low-cost early cancer detection method in the market, and cancer knowledge spreading&surveys and publicity conducted to the public.</span> | ||
+ | </p> | ||
+ | |||
+ | </p> | ||
+ | |||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Backward Arrows:What we obtained from the world---we did consultation, sample collection and surveys in medical organizations, made a cost evaluation and market demands analysis in the market and got valuable feedback and suggestions from the public on the project. </span> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | </p> | ||
+ | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">As a conclusion, we expanded the project to the practice to better serve the world with our creation, and at the same time search for suggestions from the world that could enhance our project. With the interactions between our project and the world, we felt the iGEM work this year was a big issue that influenced not only us, team NUDT_CHINA, but also people around us.</span> | ||
+ | </p> | ||
+ | </br> | ||
<h3> | <h3> | ||
− | <span><span style="color:#7f1015"> | + | <span><span style="color:#7f1015">SCHEDULE</span></span> |
</h3> | </h3> | ||
− | + | </p> | |
+ | </br></p> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:16px;">:) Click boxes above to see our project & the practice. </span> | ||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This is a specific guide of our practice work this year, listed with the time they were conducted. With our project starting and ending, the HP went on. </span> |
</p> | </p> | ||
</br> | </br> | ||
− | |||
− | |||
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− | < style | + | |
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− | </ | + | |
+ | |||
+ | |||
+ | |||
+ | |||
+ | <style> | ||
+ | .floatink{ | ||
+ | border:solid 0px red; | ||
+ | background:url("https://static.igem.org/mediawiki/2016/e/eb/Shijianzhou_NUDT_CHINA.jpg"); | ||
+ | background-size:700px 394px; | ||
+ | width:700px; | ||
+ | height:394px; | ||
+ | } | ||
+ | .floatink div{ | ||
+ | border:solid 0px red; | ||
+ | position:relative; | ||
+ | cursor:pointer; | ||
+ | } | ||
+ | #div01_floatink{ | ||
+ | left:-176px; | ||
+ | top:53px; | ||
+ | width:111px; | ||
+ | height:66px; | ||
+ | } | ||
+ | #div02_floatink{ | ||
+ | left:-60px; | ||
+ | top:-15px; | ||
+ | width:100px; | ||
+ | height:66px; | ||
+ | } | ||
+ | #div03_floatink{ | ||
+ | left:100px; | ||
+ | top:-80px; | ||
+ | width:200px; | ||
+ | height:66px; | ||
+ | } | ||
+ | #div04_floatink{ | ||
+ | left:-146px; | ||
+ | top:-25px; | ||
+ | width:90px; | ||
+ | height:60px; | ||
+ | } | ||
+ | #div05_floatink{ | ||
+ | left:-5px; | ||
+ | top:-88px; | ||
+ | width:96px; | ||
+ | height:65px; | ||
+ | } | ||
+ | #div06_floatink{ | ||
+ | left:152px; | ||
+ | top:-153px; | ||
+ | width:96px; | ||
+ | height:65px; | ||
+ | } | ||
+ | #div07_floatink{ | ||
+ | left:273px; | ||
+ | top:-216px; | ||
+ | width:96px; | ||
+ | height:65px; | ||
+ | } | ||
+ | </style> | ||
+ | <div class="center"><div class="floatink"> | ||
+ | <div id="div01_floatink" onclick="jump_ink('https://2016.igem.org/Team:NUDT_CHINA/Description')"></div> | ||
+ | <div id="div02_floatink" onclick="jump_ink('https://2016.igem.org/Team:NUDT_CHINA/Design')"></div> | ||
+ | <div id="div03_floatink" onclick="jump_ink('https://2016.igem.org/Team:NUDT_CHINA/Results')"></div> | ||
+ | <div id="div04_floatink" onclick="jump_ink('#Interviews-with-Doctors')"></div> | ||
+ | <div id="div05_floatink" onclick="jump_ink('#sample-collection-for-wet-lab')"></div> | ||
+ | <div id="div06_floatink" onclick="jump_ink('#PARTIII')"></div> | ||
+ | <div id="div07_floatink" onclick="jump_ink('#cost-evaluation')"></div> | ||
+ | </div></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <a name="PARTI"> </a> | ||
</br> | </br> | ||
+ | </br> | ||
+ | <h2 align="center" | ||
+ | > | ||
+ | <span><span style="color:#7f1015">PART I</span></span> | ||
+ | </h2> | ||
+ | <h2 align="center" | ||
+ | > | ||
+ | <span><span style="color:#7f1015">COLLABORATIONS WITH HOSPITAL & HEALTH CENTER</span></span><hr /> | ||
+ | </h2> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Considering the fact that our project this year had a close relationship with cancer, and the aim of our project was to create a novel clinical application---handy, low-cost blood-microRNA detetion system, we attached great importance to medical professionalism. Thus we turned to doctors from First Xiangya Hospital affiliated to CSU, Second Xiangya Hospital affiliated to CSU and Health Center of NUDT as our medical professional consultant in the whole process of the project.</span> |
</p> | </p> | ||
+ | <a name="Interviews-with-Doctors"> </a> | ||
</br> | </br> | ||
+ | </br> | ||
+ | <h3> | ||
+ | <span style="color:#7f1015">Interviews with Doctors</span> | ||
+ | </h3> | ||
+ | </p> | ||
+ | </br> | ||
+ | <h4> | ||
+ | <span style="color:#7f1015">1. Dr.Li Wenjin, Hematology Department, First Xiangya Affiliated Hospital of CSU</span> | ||
+ | </h4> | ||
+ | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) Procedure of Current Cancer Detection</span> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | |||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Dr.Li mentions the procedure of current cancer detection,including extracting DNA from blood cells, amplifying it with PCR and then getting its sequences.Detection varies from diseases and gene chip(detection for all genes) also could be applied with enough money.However, only some genes are of necessity to be detected for individuals.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Gap Exists Between Clinical Application and Basic Research </span> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">There was distance between clinical application and basic research in early detection of cancer. For instance, some means of early cancer detection still had a long way to go before proved efficacious---commonly 5-10 years experimental validation phase is integral to clinical application.</span></br> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(3) Stability of MiRNA in Blood is Doubted</span> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | |||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Dr.Li shows her concern about the instability of miRNA when the topic is involved with miRNA and its potential application in early detection of cancer.However,she also mentions that as a clinical doctor,she doesn’t have much research time so that she is not expert in miRNA field.Meanwhile,Dr.Li says that they clinical doctors usually pay more attention on developed technology instead of the developing one and her knowledge about miRNA comes from papers.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(4) Efficiency of MiRNA Detection is Not Guaranteed</span> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Dr.Li says that they clinician commonly assess the severity not only by the gene detection,but also by patients’ clinical symptoms,chromosome. The oncologists even need to do the Immunohistochemical checker and pathology section to make sure.So they suspect a bit about the efficiency of early cancer detection due to the lack of pathological symptoms and currently so-so specified biomarkers in patients’ early stage.</span> |
</p> | </p> | ||
</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(5) Prospect of MiRNA Is Expected</span> |
+ | </p> | ||
+ | |||
+ | |||
+ | |||
+ | |||
</p> | </p> | ||
− | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Dr.Li hopes that the team of NUDT_CHINA would find a way to detect cancer, with much greater specificity and fewer side effects, so that more targeted medicine could be invented to kill cancer tumors. |
− | </span> | + | She also figures that early cancer detection could help people carried with household hereditary disease factors.Additionally, the index of miRNA could help alert people to pay more attention to their living habits and health condition as well.</span> |
</p> | </p> | ||
</br> | </br> | ||
− | + | ||
− | < | + | <h4> |
− | <span style=" | + | <span style="color:#7f1015">2. Dr.Hou Tao, Oncology Department,Second Xiangya Affiliated Hospital of CSU</span> |
+ | </h4></span> | ||
+ | </h4> | ||
+ | |||
+ | |||
+ | |||
+ | |||
</p> | </p> | ||
</br> | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) Current situation of tumor biomarker test</span> | ||
+ | </p> | ||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We asked Dr.Hou about some details about current tumor biomarker test the hospital usuallly suggested patients to take. And this helped us learn more about the current situation of tumor biomarker test.</span> |
+ | </p> | ||
+ | |||
+ | |||
</p> | </p> | ||
</br> | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. Biomarker tests in health exams</span> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">In a normal health exam, there is an optional choice of tumor biomarker test including tests for 12 biomarkers of most frequent cancers, which is called ‘set 12’ by doctors. Talking about taking the biomarker tests, Dr.Hou said that the popularity of biomarker tests was increasing in oncology department in hospitals in China. And with more people getting familiar with tumor biomarker test, it was gaining increasing attention, and a great number of healthy people requested tumor biomarker test in their health exams, especially the elder. Tumor biomarker tests are gaining increasing attention from the crowd.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | |||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b. Details of a normal biomarker test in health exams</span> |
</p> | </p> | ||
− | |||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Contents of ‘set 12’ biomarker test in a normal health exam:</span> |
</p> | </p> | ||
− | |||
− | < | + | |
− | <span><span style="color:# | + | <p> |
− | </ | + | <table class="MsoTableGrid" style="width:414.2pt;margin:auto;"> |
+ | <tbody> | ||
+ | <tr> | ||
+ | <td width="284" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:'Arial Black';color:#000000;font-size:12pt;">CA19-9</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:'Arial Black';color:#000000;font-size:12pt;">Beta-HCG</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="260" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="margin-left:0.0000pt;text-align:center;"> | ||
+ | <span style="font-family:'Arial Black';color:#000000;font-size:12pt;">CA125</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="284" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:12pt;">NSE</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:12pt;">AFPHY</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="260" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="margin-left:0.0000pt;text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:12pt;">Ferritin</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="284" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:12pt;">CEA</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:12pt;">Free-PSA</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="260" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:12pt;">HGH</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="284" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:12pt;">CA242</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:12pt;">PSA</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="260" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="p" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:12pt;">CA15-3</span><span style="font-family:宋体;color:#C00000;font-size:12.0000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <br /> | ||
+ | <span style="background-color:;"><span style="color:#000000;"></span></span> | ||
+ | </p> | ||
+ | <p> | ||
+ | <br /> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Cost of the test for 12 biomarkers, or the ‘set 12’ is ¥500, including biomarkers of breast cancer, liver cancer and so on. Dr.Hou also told us that the validness of biomarkers was not so optimistic, thus could only work as a reference for diagnosis of cancers.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | </p> | ||
+ | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Advice for The Project---SPLIT-GFP, SPLIT LUCIFERASE, OR SPLIT HRP?</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | |||
+ | </p> | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">To improve the sensitivity and specificity of RCA output signal and to visualize the RCA outputs in the wet-lab experiments, we designed a single guide RNA mediated dCas9 binding system and a conjugated reporting system. However, dilemma came that split-luciferase, split-HRP and split-GFP all seemed to meet our requirements of reporting the signal. Which was the best fit? </span> |
+ | </p> | ||
+ | |||
+ | |||
</p> | </p> | ||
</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Firstly we searched for information of the three reporting systems:</span> |
+ | </p> | ||
+ | <table class="MsoNormalTable" align="center" style="width:435.85pt;margin:auto;"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td width="161" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Calibri;color:#C00000;font-size:10.5000pt;"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="246" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">C</span><span style="font-family:Arial;color:#000000;font-size:10.5pt;">olor</span><span style="font-family:Arial;color:#000000;font-size:10.5pt;"> reaction principle</span><span style="font-family:Calibri;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="463" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Requirements for color reaction</span><span style="font-family:宋体;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="161" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">HRP</span><span style="font-family:Calibri;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="246" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Catalyze TMB to change color</span><span style="font-family:Calibri;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="463" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Protoheme as cofactor</span><span style="font-family:宋体;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="161" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">GFP</span><span style="font-family:Calibri;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="246" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Absorbs light at a certain length and fluoresces</span><span style="font-family:宋体;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="463" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Light at a certain length as motivator</span><span style="font-family:宋体;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Fluorescence microscope</span><span style="font-family:宋体;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="161" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Luciferase</span><span style="font-family:Calibri;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="246" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Catalyse luciferin and make it fluorence</span><span style="font-family:宋体;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="463" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Fluorescence microscope</span><span style="font-family:宋体;color:#C00000;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
</p> | </p> | ||
</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Then we turned to our medical consultant Dr.Hou and asked him about suggestions of our reporting system. </span> |
</p> | </p> | ||
</br> | </br> | ||
− | </ | + | |
+ | </html> | ||
+ | [[File:侯医生采访_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html> | ||
+ | |||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We analyzed the situation together, had a talk about our novel blood-microRNA detection system, and discussed the disadvantages of current early cancer detection methods as well as expected new points of our project. Finally this helped us decide which reporting system we would use---the split-HRP.</span> |
+ | </p> | ||
+ | </br> | ||
+ | <h3> | ||
+ | <span style="color:#7f1015">Survey In The Hospital & Health Center | ||
+ | </span> | ||
+ | </h3> | ||
</p> | </p> | ||
</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We did surveys in Second Xiangya Hospital affiliated to CSU and Health Center of NUDT about details of current early cancer detection methods, which acted as a reference to our project. Here is a summary.</span> |
+ | </p> | ||
+ | <p> | ||
+ | <table class="MsoTableGrid" align="center" style="width:526.95pt;margin:auto;"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td width="123" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">METHOD</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="118" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">COST</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">(RMB)</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="150" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">TIME</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="199" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">ADVANTAGE</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="223" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">DISADVANTAGE</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="239" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">POPULARITY</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="123" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">PET-CT</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="118" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">7000-8000</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="150" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">2-3h</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="199" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Detection for early and advanced tumor</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">No pain</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Valid</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="justify" style="text-align:justify;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="223" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Expensive</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Rare</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Radiation</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Condition requirements</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">(not fit for remote </span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;"> areas)</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#0000FF;font-size:10.5pt;"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="239" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">270</span><i><span style="font-family:Arial;font-size:10.5pt;"> </span></i><span style="font-family:Arial;font-size:10.5pt;">in the whole country</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:8pt;">(data source:</span><i><span style="font-family:Arial;font-size:8pt;">2011-2015 National PET-CT Plan </span></i><span style="font-family:Arial;font-size:8pt;">by Chinese Department of Health)</span><span style="font-family:宋体;color:#0000FF;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="123" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">CT</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="118" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">200-500</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="150" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">20min</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="199" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Most effective for tumors in nervous system, head, chest and abdomen</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="223" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Only functions on a certain part</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Radiation</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Condition restriction</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="239" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Rare in counties and remote areas</span><span style="font-family:宋体;color:#0000FF;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="123" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Tumor biomarker test</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="118" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">500</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:8pt;">(including 12 biomarkers)</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="150" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">10min</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:8pt;">(sample gathering)</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="199" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Easy to operate</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">No side effects</span><span style="font-family:宋体;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="223" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Requirements for instruments</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Low validness</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">Narrow testing range</span><span style="font-family:宋体;font-size:10.5pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="239" valign="center" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;font-size:10.5pt;">City or central hospital</span><span style="font-family:宋体;color:#333333;font-size:7pt;background:#FFFFFF;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <span style="font-family:Arial;"></span> | ||
+ | </p> | ||
+ | |||
</p> | </p> | ||
</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Conclusion drawn from the result:</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | |||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. Costs of current early cancer detection methods vary largely, but on the whole the price is not low, which may make it hard for poor families to afford. Plus, it may also turn down people’s positivity to take early cancer detection.</span> |
</p> | </p> | ||
− | + | ||
− | + | ||
− | + | </p> | |
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b. These test methods have high need of good external condition, eg. Electricity, instruments, operation environment. These may restrict the early cancer detection in big cities or counties, leaving remote area aside.</span> |
+ | </p> | ||
+ | |||
+ | |||
</p> | </p> | ||
− | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Popularity of these test methods is low, which we can see from the result that people almost can take early cancer detection only in developed cities or counties. This makes it hard for people in remote areas, such as rural districts and behindhand villages, where cancer occurrence rate is also high and cancer death rate is even higher because of backward medical services.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | </p> | ||
+ | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">From these we can tell that a novel early cancer detection method that is more convenient, cheaper and has less requirements for environment is in great need. And this is exactly what our project this year is striving for. </span> |
</p> | </p> | ||
+ | <a name="sample-collection-for-wet-lab"> </a> | ||
</br> | </br> | ||
+ | </br> | ||
+ | <h3> | ||
+ | <span style="color:#7f1015">Sample Collection for Wet Lab</span> | ||
+ | </h3> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> |
</p> | </p> | ||
− | + | </br> | |
+ | </p> | ||
+ | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Since our project this year was to develop a blood-microRNA detection system as a novel method for early cancer detection, and results of previous wet lab all confirmed the effect of our detection system, it was necessary to apply it to serum as a final confirmation. We came to the health exam center of Affiliated Hospital of NUDT and talked with doctors and 5 non-small cell lung cancer(NSCLC) patients about our need for serum samples to and got their support and permission. Also we had 30 healthy postgraduates from NUDT as our volunteer of serum samples. And we collected the blood samples with the help of doctors.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1. Approve of the Informed Consent Form</span> | ||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We submitted the Informed Consent Form to the department of ethics in NUDT. After days of checking it was approved to use.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2. Consultation with doctors</span> | ||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Firstly we found Dr.Tan, one of the principles in the department of clinical lab of the health center. He introduced the formal process of collecting blood samples to us and approved our Informed Consent Form. Dr.Tan also helped us with the collecting work.</span> |
− | </span> | + | |
</p> | </p> | ||
</br> | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">3. Meeting with sample groups</span> | ||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We talked to 5 cancer patients in different stages of NSCLC as well as 30 healthy volunteers. We introduced what we had been working on and explained what we would do with their blood samples. We also made a security guarantee that we would keep their personal information as secret.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">4. Processes of sample gathering</span> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. Determination of sample group of patients and healthy volunteers and register their information.</span> | ||
+ | </p> | ||
− | < | + | <p style="text-indent:22pt;"> |
− | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b. Filling in Informed Consent Form and diagnostic information.</span> | |
− | </ | + | </p> |
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Doctors collected 5L blood sample/person with coagulation tube and match each with the person’s information(including name, age and extra information as AD, stages of NSCLC, etc from cancer patients).</span> |
</p> | </p> | ||
− | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">d. Samples transmitted to professional operators and serum was separated.</span> |
</p> | </p> | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">e. Registering samples in the information management system and setting up links with the Informed Consent Form.</span> |
</p> | </p> | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">f. Sample serum was transmitted to cryopreserve tubes and placed in -80℃ in the lab.</span> |
</p> | </p> | ||
− | </br> | + | <a name="PARTII"> </a> |
+ | </br></br> | ||
+ | <h2 align="center" | ||
+ | > | ||
+ | <span><span style="color:#7f1015">PART II</span></span> | ||
+ | </h2> | ||
+ | <h2 align="center" | ||
+ | > | ||
+ | <span><span style="color:#7f1015">PRODUCT EVALUATION</span></span><hr /> | ||
+ | |||
+ | <h3> | ||
+ | <span style="color:#7f1015">Market Demands</span> | ||
+ | </h3> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Previously we did surveys in hospitals and health center about current early cancer detection methods. To gather more information, we searched the Internet about the same issue. Here is the summary.</span> |
</p> | </p> | ||
+ | |||
+ | </html> | ||
+ | [[File:biaoge_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html> | ||
+ | |||
+ | |||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Looking at both results we found that the biggest disadvanges of current early cancer detection were costs and side effects. And this informed us of the direction our project should strive for---handy and low cost. Thus we made a cost evaluation for our product-to-be. Here are the results.</span> | ||
+ | </p><a name="cost-evaluation"> </a> | ||
</br> | </br> | ||
− | + | </br> | |
− | + | ||
− | + | ||
<h3> | <h3> | ||
− | + | <span style="color:#7f1015">Cost Evaluation</span> | |
</h3> | </h3> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We made an evaluation of efficiency and price of our detection system in this part to see how much our product may decrease the cost of biomarker detection for cancer, or to say how much our project could help the world with.</span> | ||
+ | </p> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The other reason we made this cost evaluation was to make it a feedback for the project and find other aspects it may develop itself in.</span> |
</p> | </p> | ||
</br> | </br> | ||
− | < | + | <!--此处开始链接--> |
− | + | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The following is the evaluation of our expected product cost.(Click here to view details of contents, instrument, reagents and experimental procedure of our potential product.)</span> |
</p> | </p> | ||
+ | |||
+ | |||
</br> | </br> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1. Project Contents</span> |
</p> | </p> | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">miRNA extraction; Incubation from protein and sgRNA; Fluorescence coloration</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2. Major Instruments and Reagents</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) Instruments</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Centrifugal machine, Freeze drying machine, 37 degree constant temperature box, Filter paper and other laboratory supplies, Computers and printers</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Reagents</span> |
</p> | </p> | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Ribonuclease inhibitor, Double distilled water, Special probe , Phi29 DNA polymerase, Buffer phi29, BSA, dNTP, Cas9 protein,and sgRNA</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">3. Experimental Procedure</span> |
</p> | </p> | ||
− | + | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Firstly,freeze-dry the required reagents (such as enzymes, proteins, buffer, etc.) in a solid carrier (filter paper, etc.).</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Then,get serum from provided blood, boil to destroy binding between miRNA and protein and extract miRNA.</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Finally,add extracted miRNA to the solid carrier and wait for coloration,compare with the standard color card to estimate contents of miRNA.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | <!--链接结束--> | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">1. Cost Evaluation</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | <p> | ||
+ | <table class="MsoTableGrid" style="width:426.1pt;margin:auto;"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td width="230" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">name of reagents</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="195" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Unit price</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Use level</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Equivalent</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="230" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Ribonuclease inhibitor</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="195" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$32.2/200ml</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">37.6ul</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.006</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="230" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">phi29 DNA polymerase</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="195" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$70.5/250U(10U/ul)</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">0.6ul</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$1.692</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="230" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Buffer phi29</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="195" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$19.5/5ml</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">3ul</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.012</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="230" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">dNTP</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="195" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$12.0/1ml</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">7.2ul</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.086</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="230" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Special probe</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="195" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.16</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">one</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.163</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="230" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Cas9 protein</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="195" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$22.3/g</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">1ul(0.2uM)</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.713</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="230" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">double distilled water</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="195" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$3.0/25kg</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">491.2ul</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="213" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">$0.000</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </p> | ||
+ | |||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Assuming that the protein can be made to the level of industrial production, the total reagent cost is about $2.672.</span> |
</p> | </p> | ||
− | </ | + | <p> |
+ | <table class="MsoTableGrid" style="width:426.1pt;margin:auto;"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">Name of equipment</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">Business</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">Price</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">centrifugal machine</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">TGL-16 high speed centrifuge (1.5ml*12 hole)</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">$77.979</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">Freeze dryer</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">LY-15AC high efficiency freezing and drying machine</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">$450.224</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;"> </span> | ||
+ | </p> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">Constant temperature box</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">DH-250AB electric heating constant temperature incubator</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">$335.908</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">HP printers</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">HP color laser integrated machine</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="284" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal"> | ||
+ | <span style="font-family:Arial;color:#7030A0;font-size:10.5pt;">$427.382</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <span style="font-family:Arial;"></span> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">All of these machines can be used for long and we don’t need to take them while testing miRNA outside. So we consider loss only. Centrifugal machine is used to extract serum while freeze dryer is used to freeze-dry relative reagents in the test paper. We apply HP color laser integrated machine to make colorimetric card.Briefly,the loss price is about $0.3.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">2. Interpretation of Result</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) The total price of our project is around $3.</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">During the experiment, we found that this method can achieve requirements of high sensitivity, low cost and easy operation.</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) Comparisons with some other quantitative miRNA detection methods.</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. miRNA Real-time qPCR</span> |
</p> | </p> | ||
− | </br> | + | |
+ | |||
+ | |||
+ | <p style="text-align:center;"> | ||
+ | <br /> | ||
+ | </p> | ||
+ | <p> | ||
+ | <table class="MsoNormalTable" style="text-align:center;width:414.95pt;margin:auto;"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td width="249" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">project</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="243" valign="center" colspan="2" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">contents</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="189" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <b><span style="font-family:Arial;color:#000000;font-size:10.5pt;">price</span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="147" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">period</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="249" valign="center" rowspan="3" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">miRNA SYBR Green I Relative quantification</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="102" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Basic cost</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="140" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Primer synthesis</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="189" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <b><span style="font-family:Arial;color:#000000;font-size:10.5pt;">$223.2/miRNA</span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="147" valign="center" rowspan="3" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">4-5weeks</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="102" valign="center" rowspan="2" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Sample detection fee</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="140" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"><span style="font-family:Arial;color:#000000;">number</span><span style="font-family:Arial;color:#000000;"><</span><span style="font-family:Arial;color:#000000;">10</span></span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="189" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"><span style="font-family:Arial;color:#000000;">$44.6/sample</span><span style="font-family:Arial;color:#000000;">·</span><span style="font-family:Arial;color:#000000;">gene</span></span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="140" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"><span style="font-family:Arial;color:#000000;">number</span><span style="font-family:Arial;color:#000000;">≥</span><span style="font-family:Arial;color:#000000;">10</span></span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="189" valign="center" style="border:1px solid windowtext;background:#FFFFFF;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"><span style="font-family:Arial;color:#000000;">$29.8/sample</span><span style="font-family:Arial;color:#000000;">·</span><span style="font-family:Arial;color:#000000;">gene</span></span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <br /> | ||
+ | <br /> | ||
+ | </p> | ||
+ | <div style="text-align:center;"> | ||
+ | <span style="line-height:1.5;"></span> | ||
+ | </div> | ||
+ | <p> | ||
+ | <br /> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | |||
+ | </p> | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">miRNA CHIP</span> |
</p> | </p> | ||
− | </ | + | |
+ | <table class="MsoTableGrid" style="width:414.6pt;margin:auto;"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td width="320" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">C</span><span style="font-family:Arial;color:#000000;font-size:10.5pt;">HIP</span><span style="font-family:Arial;color:#000000;font-size:10.5pt;"> </span><span style="font-family:Arial;color:#000000;font-size:10.5pt;">N</span><span style="font-family:Arial;color:#000000;font-size:10.5pt;">ame</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="171" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">miRNA</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="172" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Probe </span><span style="font-family:Arial;color:#000000;font-size:10.5pt;">R</span><span style="font-family:Arial;color:#000000;font-size:10.5pt;">epetition</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="165" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <b><span style="font-family:Arial;color:#E53333;font-size:10.5pt;">Price</span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="320" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">RiboArrayTM Human Array 1*12K</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="171" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">2588</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="172" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">3</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="165" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <b><span style="font-family:Arial;color:#E53333;font-size:10.5pt;">$595.3</span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="320" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">Human brain cancer miRNA PCR Array</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="171" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">84</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="172" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">\</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="165" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <b><span style="font-family:Arial;color:#E53333;font-size:10.5pt;">$714.4</span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="320" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">TaqMan® Array Human microRNA Panel V2.0</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="171" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="justify" style="text-align:justify;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;"> 667</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="172" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">8</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="165" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <b><span style="font-family:Arial;color:#E53333;font-size:10.5pt;">$892.9</span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="320" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"><span style="font-family:Arial;color:#000000;">miRCURY LNA</span><span style="font-family:Arial;color:#000000;">™ </span><span style="font-family:Arial;color:#000000;">Universal RT microRNA PCR Array</span></span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="171" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="justify" style="text-align:justify;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;"> \</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="172" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <span style="font-family:Arial;color:#000000;font-size:10.5pt;">\</span><span style="font-family:宋体;color:#7030A0;font-size:10.5000pt;"></span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="165" valign="top" style="border:1.0000pt solid windowtext;"> | ||
+ | <p class="MsoNormal" align="center" style="text-align:center;"> | ||
+ | <b><span style="font-family:Arial;color:#E53333;font-size:10.5pt;">$297.6</span></b><b><span style="font-family:宋体;color:#FF0000;font-size:10.5pt;"></span></b> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">From the comparisons, we can easily draw the conclusion that:</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. Our product-to-be costs only $3 in each round, it has an overwhelming advantage in cost.</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b. The reporting system of our project is the split-HRP, which satisfied the need of a new visible signal showing method. Thus, we eliminated heavy instruments and strict environment requests, finally making it possible to put it into wide use in remote areas which lack qualified conditions for current early cancer detection methods.</span> |
</p> | </p> | ||
+ | |||
+ | |||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Our project is expected to solve the problem of lack of early cancer detection methods in remote areas as well as places bad in medical conditions.</span> | ||
+ | </p><a name="PARTIII"> </a> | ||
</br> | </br> | ||
+ | </br> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <h2 align="center" | ||
+ | > | ||
+ | <span><span style="color:#7f1015">PART III</span></span> | ||
+ | </h2> | ||
+ | <h2 align="center" | ||
+ | > | ||
+ | <span><span style="color:#7f1015">RAISING AWARENESS</span></span><hr /> | ||
+ | |||
+ | |||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">In the project this year, the aim of our creation of the product was to detect blood-microRNA thus to promote early cancer detection and then to help the world. </span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">However, to realize helping the world, this is far from enough. Thus we scheduled a series of Human Practice works for all ages to expand our project and ideas out of the lab---we raised people’s awareness of cancer&early cancer detection.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | <h3> | ||
+ | <span style="color:#7f1015">Internet Survey For The Public</span> | ||
+ | </h3> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">In this survey about cancer and early cancer detection, we we chose netizens as our respondent, thus the results would be more objective and random. And we may get more comprehensive and true answers. We designed an Internet survey and put it on social media. Until we stopped the Internet survey, there were 524 netizens who took part in it.</span> |
</p> | </p> | ||
− | < | + | |
+ | <!---此处链接---> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The survey mainly involved the three aspects:</span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(1) How much the public knew about early cancer detection.</span> |
</p> | </p> | ||
− | </ | + | |
+ | </html> | ||
+ | [[File:diaocha1_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(2) What their main problems were when seeking for early cancer detection.</span> |
</p> | </p> | ||
</br> | </br> | ||
+ | |||
+ | </html> | ||
+ | [[File:图片2_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">(3) To publize knowledge of cancer,early cancer detection and our project</span> |
</p> | </p> | ||
− | + | ||
+ | |||
+ | |||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">a. What do you think of our project?</span> |
</p> | </p> | ||
− | </ | + | </html> |
+ | [[File:diaocha3_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">b. What do you think is acceptable of our project?</span> |
</p> | </p> | ||
− | </ | + | </html> |
+ | [[File:diaocha4_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">c. Do you expect to see our product put in to market?</span> |
</p> | </p> | ||
− | </ | + | </html> |
+ | [[File:diaocha5 NUDT CHINA.jpg|700px|center]] | ||
+ | <html> | ||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">The data from this survey reflected that although most people attached great importance to early cancer detection, not so many of them knew much about cancer detection, which reminded us that if we wanted to help the world with our handy tumor biomarkers detection system, lateral publicity of early cancer detection to arouse people’s awareness would play a vital role. </span> |
</p> | </p> | ||
− | + | ||
+ | |||
<p style="text-indent:22pt;"> | <p style="text-indent:22pt;"> | ||
− | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | + | <span style="line-height:2;font-family:Perpetua;font-size:18px;">What’s more, people had the idea to take early cancer detection, but were stopped by the high cost, possible radiation and operating complexity of current methods. Thus our handy, low-cost detection system may have great hope to decrease the pressure of high cost and complex steps of current early cancer detection methods.</span> |
+ | </p> | ||
+ | |||
+ | |||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We also learned that people held great expectations towards our project. However, validness of our product were what they doubted most, which was also a common problem of almost all biomarker tests. This reminded us that we must try to raise the validness of the product in our lataral work.</span> | ||
</p> | </p> | ||
</br> | </br> | ||
+ | <h3> | ||
+ | <span style="color:#7f1015">Ethics</span> | ||
+ | </h3> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Since the idea this year has a close relationship with cancer early detection, we had a discussion standing in shoes of cancer patients. | ||
+ | (click to view details of our discussion)</span> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Here are some typical topics:</span> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Q1: What problems cancer patients may meet with in their life?</span> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Q2: Should patients with advanced tumor stop the treatment?</span> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Q3: Whether women should remove breast as they have mammary cancer and couldn’t have conservative operation?</span> | ||
+ | </p> | ||
+ | </br> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We held this discussion in the laboratory, and called on several students from different majors(eg. Chemistry, Medicine, Electronic Engineering) to take part. As we talked gradually deeper, our moods became increasingly low. Cancer has been a monster for so many years, but the ethical issues of treatment, finance, lie or tell and social assistance have never had an answer.</span> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This year what we want to spread is not only a novel early cancer detection system, but also awareness of people with cancer. We are all weak in the face of diseases, especially deadly ones like cancer. Only if we pay attention to our health and stay alert for accidental diseases can we live a healthy life for long.</span> | ||
+ | </p> | ||
+ | </br> | ||
+ | <h3> | ||
+ | <span style="color:#7f1015">Grandpas and Grannys’ Mini Lecture </span> | ||
+ | </h3> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We planned mini lectures of knowledge of cancer and early cancer detection in several districts mainly for the elder to increase their attention to early cancer detection. Also we wanted to spread the idea that early cancer was not terrifying and could be cured or prevented. Finally , we publicized knowledge about synthetic biology, iGEM, and our project this year.</span> | ||
+ | </p> | ||
+ | </br> | ||
+ | </html> | ||
+ | [[File:社区1_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html> | ||
+ | </br></br> | ||
+ | </html> | ||
+ | [[File:社区2_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html></br></br> | ||
+ | </html> | ||
+ | [[File:社区3_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html></br></br> | ||
+ | </html> | ||
+ | [[File:社区4_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html></br></br> | ||
+ | <h3> | ||
+ | <span style="color:#7f1015">Lab Day For High School Students </span> | ||
+ | </h3> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">We invited several high school students who were enthusiastic about biology and their teachers from Changsha No.1 High School to our laboratory. | ||
+ | As for the activities, firstly we made a presentation about synthetic biology, iGEM and our project. Then we showed these guests around our lab and introduced our instruments and experiments, and guided them to carry out some interesting biology experiments including polymerase digest and electrophoresis.</span> | ||
+ | </p> | ||
+ | </br> | ||
+ | </html> | ||
+ | [[File:实验室1_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html> | ||
+ | </br></br> | ||
+ | </html> | ||
+ | [[File:实验室2_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html></br></br> | ||
+ | </html> | ||
+ | [[File:实验室3_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html></br> | ||
+ | </br> | ||
+ | <h3> | ||
+ | <span style="color:#7f1015">Fun Game with Our Topic</span> | ||
+ | </h3> | ||
+ | </br</br><p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This is a game video with topic of our project cast by a player, one of our game tester.We built this game and spread it to people around us. Everyone was surprised about it. </span> | ||
+ | </p> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">This is a game video with topic of our project cast by a player, one of our game tester.We built this game and spread it to people around us. Everyone was surprised about it. </span> | ||
+ | </p> | ||
+ | </br><video style="width:100%;" controls="controls" preload="auto"> | ||
+ | <source src="https://static.igem.org/mediawiki/2016/d/d5/T--NUDT_CHINA--Game_Video.mp4" type="video/mp4" /> | ||
+ | Your browser does not support the video tag. Please click <a href="https://static.igem.org/mediawiki/2016/d/d5/T--NUDT_CHINA--Game_Video.mp4">HERE</a> to open this video! | ||
+ | </video> | ||
+ | <a href="">https://static.igem.org/mediawiki/2016/d/d5/T--NUDT_CHINA--Game_Video.mp4</a> | ||
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+ | |||
+ | |||
+ | |||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">Adventure of iGEM is a third person adventure game that aims at introducing iGEM and our project this year to everyone in an interesting way.</span> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">A normal student named Itazk joins the team of iGEM. One day he falls asleep in the lab and has a dream. In this dream, he must work out some puzzles to find artifacts of NUDT’s blood-microRNA detection system and defeat Monster Cancer.</span> | ||
+ | </p> | ||
+ | </br> | ||
+ | <h3> | ||
+ | <span style="color:#7f1015">Long-term sustainability</span> | ||
+ | </h3> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;">To help our project leave a deep impression on people, also to expand the range of our publicity, we made useful little things with knowledge of cancer/early cancer detection/iGEM for families as souveniors.</span> | ||
+ | </p> | ||
+ | </html> | ||
+ | [[File:扇子图案_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html> | ||
+ | <p> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:16px;">This is the mini poster about knowledge of cancer and early cancer detection for everyone. We put it on small fans and gave them away to the crowd.</span> | ||
+ | </p> | ||
+ | </br> | ||
+ | </html> | ||
+ | [[File:发扇子_NUDT_CHINA.jpg|700px|center]] | ||
+ | <html> | ||
+ | <p style="text-indent:22pt;"> | ||
+ | <span style="line-height:2;font-family:Perpetua;font-size:18px;"></span> | ||
+ | </p> | ||
+ | </br> | ||
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− | + | <div style="border:solid 1px rgb(10,31,84);background-color:rgb(10,31,84);"> | |
− | + | <div class="row footer-link" style="border:solid 0px red;margin-top:100px;"> | |
− | + | <style>.menu-link-container ul li{ list-style:none; } .menu-link-container a{ color:white; font-size:14px;line-height:26px; } | |
− | + | .menu-link-container a:hover{ | |
− | + | text-decoration:underline; | |
− | + | } #c_menu9909 .medium-6{ text-align:center; width:auto; border:solid 0px red; } #c_menu9909 .medium-6 h5{color:white;}#c_menu9909 .medium-6{height:315px;border:solid 0px red;}</style> | |
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− | + | <div class="menu-link-container"></div></div--> | |
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− | + | <a href="/Team:NUDT_CHINA/Team">Team</a><br/> | |
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− | + | <a href="/Team:NUDT_CHINA/Collaborations">Collaborations</a><br/> | |
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− | + | ||
</div> | </div> | ||
+ | <div class="medium-6 columns" style="width:auto;"> | ||
+ | <h5 class="column-header">PROJECT</h5> | ||
+ | <div class="menu-link-container"> | ||
+ | |||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Description">Description</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Design">Design</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Experiments">Experiments</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Proof">Proof of Concept</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Demonstrate">Demonstrate</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Results">Results</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Notebook">Notebook</a><br/> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="medium-6 columns" style="width:auto;"> | ||
+ | <h5 class="column-header">PARTS</h5> | ||
+ | <div class="menu-link-container"> | ||
+ | |||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Parts">Parts</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Basic_Part">Basic Parts</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Composite_Part">Composite Parts</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Part_Collection">Part Collection</a><br/> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="medium-6 columns" style="width:auto;"> | ||
+ | <a href="/Team:NUDT_CHINA/Safety" style="text-decoration:none;"> | ||
+ | <h5 class="column-header">SAFETY</h5></a> | ||
+ | |||
+ | </div> | ||
+ | <div class="medium-6 columns" style="width:auto;"> | ||
+ | <a href="/Team:NUDT_CHINA/Attributions" style="text-decoration:none;"> | ||
+ | <h5 class="column-header">ATTRIBUTIONS</h5></a> | ||
+ | |||
+ | </div> | ||
+ | <!--div class="medium-6 columns" style="width:auto;"> | ||
+ | <A href="/Team:NUDT_CHINA/Attributions" style="text-decoration:none;"><h5 class="column-header">ATTRIBUTIONS</h5></A> | ||
+ | <div class="menu-link-container"></div></div--> | ||
+ | <div class="medium-6 columns" style="width:auto;"> | ||
+ | <h5 class="column-header">HUMAN | ||
+ | <br>PRACTICES</h5> | ||
+ | <div class="menu-link-container"> | ||
+ | |||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Human_Practices">Human Practices</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/HP/Silver">Silver</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/HP/Gold">Gold</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Integrated_Practices">Integrated Practices</a><br/> | ||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Engagement">Engagement</a><br/> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="medium-6 columns" style="width:auto;"> | ||
+ | <h5 class="column-header">AWARDS</h5> | ||
+ | <div class="menu-link-container"> | ||
+ | |||
+ | |||
+ | <a href="/Team:NUDT_CHINA/Model">Model</a><br/> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="medium-6 columns" style="width:0px;height:0px;"></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
<footer id="footer"> | <footer id="footer"> | ||
<div class="row footer-link"> | <div class="row footer-link"> | ||
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--> | --> | ||
− | + | <!-- to top edit by inksci---> | |
+ | <style> | ||
+ | #to_top{ | ||
+ | position:fixed; | ||
+ | bottom:100px; | ||
+ | right:0px; | ||
+ | width:60px; | ||
+ | height:60px; | ||
+ | font-size:14px; | ||
+ | line-height:60px; | ||
+ | text-align:center; | ||
+ | background-color:black; | ||
+ | color:rgb(246,168,0); | ||
+ | cursor:pointer; | ||
+ | } | ||
+ | </style> | ||
+ | <div onclick="top_it()" id="to_top"> | ||
+ | TOP | ||
+ | </div> | ||
+ | <script type="text/javascript"> | ||
+ | var oTop = document.getElementById("to_top"); | ||
+ | oTop.style.display="none"; | ||
+ | var top_break=false; | ||
+ | /** | ||
+ | * 回到页面顶部 | ||
+ | * @param acceleration 加速度 | ||
+ | * @param time 时间间隔 (毫秒) | ||
+ | **/ | ||
+ | var save_scrolltop=100000000000000; | ||
+ | window.onscroll = function(){ | ||
+ | |||
+ | |||
+ | var scrolltop = document.documentElement.scrollTop || document.body.scrollTop; | ||
+ | if(scrolltop>300){ | ||
+ | oTop.style.display="block"; | ||
+ | }else{ | ||
+ | oTop.style.display="none"; | ||
+ | } | ||
+ | if(scrolltop>save_scrolltop) | ||
+ | { | ||
+ | top_break=true; | ||
+ | } | ||
+ | save_scrolltop=scrolltop; | ||
+ | } | ||
+ | function top_it(){ | ||
+ | top_break=false; | ||
+ | goTop();return false; | ||
+ | } | ||
+ | function goTop(acceleration, time) { | ||
+ | if(top_break){ | ||
+ | return; | ||
+ | } | ||
+ | oTop.style.display="none"; | ||
+ | acceleration = acceleration || 0.1; | ||
+ | time = time || 16; | ||
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+ | if (document.documentElement) { | ||
+ | x1 = document.documentElement.scrollLeft || 0; | ||
+ | y1 = document.documentElement.scrollTop || 0; | ||
+ | } | ||
+ | if (document.body) { | ||
+ | x2 = document.body.scrollLeft || 0; | ||
+ | y2 = document.body.scrollTop || 0; | ||
+ | } | ||
+ | var x3 = window.scrollX || 0; | ||
+ | var y3 = window.scrollY || 0; | ||
+ | |||
+ | // 滚动条到页面顶部的水平距离 | ||
+ | var x = Math.max(x1, Math.max(x2, x3)); | ||
+ | // 滚动条到页面顶部的垂直距离 | ||
+ | var y = Math.max(y1, Math.max(y2, y3)); | ||
+ | |||
+ | // 滚动距离 = 目前距离 / 速度, 因为距离原来越小, 速度是大于 1 的数, 所以滚动距离会越来越小 | ||
+ | var speed = 1 + acceleration; | ||
+ | window.scrollTo(Math.floor(x / speed), Math.floor(y / speed)); | ||
+ | |||
+ | // 如果距离不为零, 继续调用迭代本函数 | ||
+ | if(x > 0 || y > 0) { | ||
+ | var invokeFunction = "goTop(" + acceleration + ", " + time + ")"; | ||
+ | window.setTimeout(invokeFunction, time); | ||
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+ | <!-- to top edit by inksci---> | ||
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Latest revision as of 03:54, 20 October 2016
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